Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   R6U80_RS06345 Genome accession   NZ_CP137623
Coordinates   1290855..1291703 (+) Length   282 a.a.
NCBI ID   WP_012897825.1    Uniprot ID   A0A2A9I6Y4
Organism   Lactococcus lactis strain NCK401     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1285855..1296703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6U80_RS06320 (R6U80_06320) ilvN 1286612..1287088 (+) 477 WP_010905839.1 acetolactate synthase small subunit -
  R6U80_RS06325 (R6U80_06325) ilvC 1287134..1288156 (+) 1023 WP_313792046.1 ketol-acid reductoisomerase -
  R6U80_RS06330 (R6U80_06330) ilvA 1288212..1289462 (+) 1251 WP_017864495.1 threonine ammonia-lyase IlvA -
  R6U80_RS06335 (R6U80_06335) budA 1289578..1290288 (+) 711 WP_003131140.1 acetolactate decarboxylase -
  R6U80_RS06340 (R6U80_06340) - 1290329..1290709 (+) 381 WP_003131142.1 RidA family protein -
  R6U80_RS06345 (R6U80_06345) dprA 1290855..1291703 (+) 849 WP_012897825.1 DNA-processing protein DprA Machinery gene
  R6U80_RS06350 (R6U80_06350) topA 1291965..1294097 (+) 2133 WP_313792045.1 type I DNA topoisomerase -
  R6U80_RS06355 (R6U80_06355) trmFO 1294169..1295512 (+) 1344 WP_025017071.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  R6U80_RS06360 (R6U80_06360) xerS 1295557..1296627 (-) 1071 WP_003131037.1 tyrosine recombinase XerS -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 32119.17 Da        Isoelectric Point: 9.5086

>NTDB_id=899397 R6U80_RS06345 WP_012897825.1 1290855..1291703(+) (dprA) [Lactococcus lactis strain NCK401]
MITNFDLYRWKKAGMTNLGVNKLLKFFHRYDKKISLRQMGQVAQLKSIPNFIEQYKNQDVSKLREEYKIYPSFSLLDEIY
PERLKEIYNPPVLLFYQGDIRLLKTPKLAFVGSRQASSQGIKAVQKIVTELNQNFTIVSGLAKGIDTASHLSAIKNKIPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLILSEYSVGEKPLRYHFPERNRIIAGLSHGVVVIEAKLRSGSLITCERALEEG
RDIFAVPGNIADGFSDGCNHLIQQGAKLIYQAQDILEEYLYD

Nucleotide


Download         Length: 849 bp        

>NTDB_id=899397 R6U80_RS06345 WP_012897825.1 1290855..1291703(+) (dprA) [Lactococcus lactis strain NCK401]
ATGATAACAAATTTTGATTTATATCGTTGGAAAAAAGCTGGCATGACCAATTTAGGAGTAAATAAACTTTTAAAATTTTT
TCATAGATATGATAAAAAAATAAGTTTAAGACAAATGGGACAAGTAGCACAACTTAAATCAATTCCCAACTTTATTGAAC
AATATAAAAATCAAGACGTAAGTAAACTGAGAGAAGAGTATAAAATATACCCATCATTTTCTCTTCTTGATGAAATTTAT
CCCGAAAGACTCAAGGAAATTTACAATCCACCGGTTCTATTATTTTATCAAGGAGATATAAGACTCTTAAAAACTCCTAA
ATTAGCTTTTGTTGGTAGTCGACAAGCAAGCTCACAAGGAATTAAAGCCGTTCAAAAAATTGTGACAGAGTTGAACCAAA
ATTTTACGATTGTCAGCGGACTCGCTAAAGGAATTGATACAGCAAGTCATCTTTCTGCCATTAAAAATAAAATCCCTACA
ATTGCAGTTATCGGTACAGGCTTAGATATCTTTTATCCTTTAGAAAATCGAAAAATTCAAGAGTATCTTGCTAAAAACCA
ACTCATACTATCAGAATATTCAGTCGGAGAAAAGCCACTTCGTTATCATTTTCCTGAACGTAATCGTATCATTGCGGGAC
TTTCACATGGTGTAGTAGTGATAGAAGCAAAGTTACGAAGTGGTAGTTTAATTACTTGTGAGCGCGCTCTGGAAGAAGGA
AGAGATATTTTCGCTGTACCAGGAAACATAGCAGATGGATTTTCAGATGGCTGTAATCATCTAATACAACAAGGAGCAAA
ATTAATCTATCAAGCCCAAGATATCTTAGAAGAATATTTGTATGATTAA

Domains

Predicted by InterproScan.

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(2-63)

DNA recombination-mediator protein A (DNA_processg_A)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A9I6Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Lactococcus lactis subsp. cremoris KW2

86.879

100

0.869
  dprA Streptococcus mutans UA159

60.072

98.582

0.592
  dprA/cilB/dalA Streptococcus mitis SK321

54.643

99.291

0.543
  dprA/cilB/dalA Streptococcus pneumoniae R6

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae D39

53.929

99.291

0.535
  dprA Legionella pneumophila strain ERS1305867

39.13

97.872

0.383
  dprA Haemophilus influenzae Rd KW20

38.889

95.745

0.372
  dprA Bacillus subtilis subsp. subtilis str. 168

43.777

82.624

0.362
  dprA Latilactobacillus sakei subsp. sakei 23K

40

90.426

0.362