Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   R5R33_RS04500 Genome accession   NZ_CP137555
Coordinates   995355..996569 (+) Length   404 a.a.
NCBI ID   WP_318954851.1    Uniprot ID   -
Organism   Microbulbifer pacificus strain SPO729     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 990355..1001569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5R33_RS04480 (R5R33_04480) - 990366..990512 (-) 147 WP_318954847.1 hypothetical protein -
  R5R33_RS04485 (R5R33_04485) - 990808..992538 (-) 1731 WP_318954848.1 Wzy polymerase domain-containing protein -
  R5R33_RS04490 (R5R33_04490) - 992604..993077 (-) 474 WP_318954849.1 pilin -
  R5R33_RS04495 (R5R33_04495) pilB 993568..995277 (+) 1710 WP_318954850.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R5R33_RS04500 (R5R33_04500) pilC 995355..996569 (+) 1215 WP_318954851.1 type II secretion system F family protein Machinery gene
  R5R33_RS04505 (R5R33_04505) - 996585..997469 (+) 885 WP_318954852.1 A24 family peptidase -
  R5R33_RS04510 (R5R33_04510) coaE 997483..998085 (+) 603 WP_318954853.1 dephospho-CoA kinase -
  R5R33_RS04515 (R5R33_04515) yacG 998100..998321 (+) 222 WP_318954854.1 DNA gyrase inhibitor YacG -
  R5R33_RS04520 (R5R33_04520) - 998400..998888 (-) 489 WP_318954855.1 protein disulfide oxidoreductase -
  R5R33_RS04525 (R5R33_04525) tsaA 998885..999592 (-) 708 WP_318954856.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  R5R33_RS04530 (R5R33_04530) - 999696..1001153 (-) 1458 WP_318954857.1 NAD-dependent succinate-semialdehyde dehydrogenase -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43457.17 Da        Isoelectric Point: 9.7143

>NTDB_id=899032 R5R33_RS04500 WP_318954851.1 995355..996569(+) (pilC) [Microbulbifer pacificus strain SPO729]
MANAAVATAYVFKGVDAKGNRVEGEVTGTNPTLIKAQLRRQGIIANKVQKKPKPLFGGKKKIKPGDIAVFTRQMATMMRA
GVPLVQSFEIVADGLDNAGLKDLIFKVRDDVASGTAFADALRRHPLYFDDLFCNLVASGEQSGALETMLDRIATYKEKTE
SLKAKIKKAMTYPIAVVVVAVVVTAILLVKVVPQFAETFSSFGADLPAFTLFVLSISEWMQANWFFALLALVVGIGGTLE
VKKRNKKVANFFDKLMLKLPILGQITYNSIAARYARTLSTTFAAGVPLIDALKSVAGATGNVVYEQATLRIRDAVATGIP
LNGAMRSSGLYPSMLVQMAAIGEESGALDEMLAKAADFYEEAVDNMVDNLTTLLEPMIMAVLGVLVGGLMIAMYLPIFQL
GNVV

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=899032 R5R33_RS04500 WP_318954851.1 995355..996569(+) (pilC) [Microbulbifer pacificus strain SPO729]
ATGGCAAATGCCGCCGTCGCAACTGCTTATGTATTCAAGGGTGTGGATGCCAAAGGCAACCGGGTAGAAGGTGAAGTCAC
CGGAACAAACCCTACTCTAATCAAGGCTCAATTGCGCCGCCAGGGCATAATTGCCAACAAGGTACAGAAAAAGCCCAAAC
CGCTTTTTGGTGGCAAGAAAAAAATCAAACCCGGCGATATCGCGGTTTTTACGCGGCAGATGGCCACTATGATGAGAGCA
GGGGTGCCGCTGGTACAGAGCTTTGAGATCGTGGCCGACGGCCTGGACAACGCCGGATTGAAAGATCTGATTTTCAAGGT
TCGCGATGATGTCGCATCAGGTACTGCTTTCGCCGATGCTCTGCGCAGGCACCCGCTCTACTTTGACGATCTATTCTGTA
ACTTGGTGGCATCCGGTGAACAATCCGGTGCATTGGAAACCATGTTGGATCGGATCGCTACTTACAAGGAAAAAACCGAG
TCACTCAAGGCCAAGATCAAGAAGGCCATGACTTATCCAATTGCGGTAGTAGTAGTCGCTGTTGTTGTAACCGCTATCTT
GTTGGTAAAAGTAGTGCCCCAATTTGCGGAAACCTTCTCGAGTTTCGGCGCAGATCTTCCCGCCTTTACCCTATTCGTAT
TGAGTATTTCCGAATGGATGCAGGCCAACTGGTTTTTCGCACTCCTCGCTTTAGTTGTTGGTATCGGCGGCACACTAGAA
GTCAAAAAACGCAATAAAAAGGTAGCCAATTTTTTCGACAAATTGATGCTTAAGCTTCCGATTCTCGGGCAGATCACCTA
CAACTCCATCGCCGCGCGCTATGCACGCACCCTCTCTACAACTTTTGCTGCAGGTGTACCACTAATCGATGCACTCAAAT
CTGTCGCTGGCGCTACCGGCAATGTGGTTTATGAACAGGCCACATTGAGAATCCGCGACGCAGTAGCTACCGGTATCCCA
TTGAACGGCGCCATGCGCAGCTCCGGCCTCTACCCTTCCATGCTTGTACAGATGGCAGCTATCGGTGAGGAATCCGGTGC
GCTGGACGAAATGCTCGCTAAAGCCGCAGACTTCTACGAGGAGGCTGTAGACAACATGGTCGATAACCTGACTACATTAC
TGGAACCCATGATTATGGCGGTTTTGGGGGTTCTCGTTGGCGGACTGATGATTGCCATGTACCTCCCCATCTTCCAGCTC
GGCAACGTGGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-396)

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

62.469

100

0.626
  pilC Acinetobacter baylyi ADP1

59.352

99.257

0.589
  pilC Acinetobacter baumannii D1279779

58.663

100

0.587
  pilC Legionella pneumophila strain ERS1305867

54.591

99.752

0.545
  pilG Neisseria gonorrhoeae MS11

42.677

98.02

0.418
  pilG Neisseria meningitidis 44/76-A

42.677

98.02

0.418
  pilC Vibrio campbellii strain DS40M4

40.704

98.515

0.401
  pilC Vibrio cholerae strain A1552

40.657

98.02

0.399