Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R5R33_RS04495 Genome accession   NZ_CP137555
Coordinates   993568..995277 (+) Length   569 a.a.
NCBI ID   WP_318954850.1    Uniprot ID   A0AAU0N293
Organism   Microbulbifer pacificus strain SPO729     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 988568..1000277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5R33_RS04470 (R5R33_04470) - 989221..989466 (-) 246 WP_318954845.1 YdcH family protein -
  R5R33_RS04475 (R5R33_04475) - 989639..990103 (-) 465 WP_318954846.1 pilin -
  R5R33_RS04480 (R5R33_04480) - 990366..990512 (-) 147 WP_318954847.1 hypothetical protein -
  R5R33_RS04485 (R5R33_04485) - 990808..992538 (-) 1731 WP_318954848.1 Wzy polymerase domain-containing protein -
  R5R33_RS04490 (R5R33_04490) - 992604..993077 (-) 474 WP_318954849.1 pilin -
  R5R33_RS04495 (R5R33_04495) pilB 993568..995277 (+) 1710 WP_318954850.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R5R33_RS04500 (R5R33_04500) pilC 995355..996569 (+) 1215 WP_318954851.1 type II secretion system F family protein Machinery gene
  R5R33_RS04505 (R5R33_04505) - 996585..997469 (+) 885 WP_318954852.1 A24 family peptidase -
  R5R33_RS04510 (R5R33_04510) coaE 997483..998085 (+) 603 WP_318954853.1 dephospho-CoA kinase -
  R5R33_RS04515 (R5R33_04515) yacG 998100..998321 (+) 222 WP_318954854.1 DNA gyrase inhibitor YacG -
  R5R33_RS04520 (R5R33_04520) - 998400..998888 (-) 489 WP_318954855.1 protein disulfide oxidoreductase -
  R5R33_RS04525 (R5R33_04525) tsaA 998885..999592 (-) 708 WP_318954856.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62491.50 Da        Isoelectric Point: 5.9720

>NTDB_id=899031 R5R33_RS04495 WP_318954850.1 993568..995277(+) (pilB) [Microbulbifer pacificus strain SPO729]
MSSSPLSGLAKRLVQDKAFDEATAQSAIKAAKREGHSFAQHVVEAKLIKSRELANIASVAFGTPLFDLSSYNFDLLPKGI
VDEKLITKHFALPLYRRGNRLFVAVADPTNLAGLDEINFNTGLNTDAVLVEADKLAKAIENYLNNSDVGGGLEGLDDEDL
DSLDVESGDTRREDDNEPGGDEAPVVRFVNKVLLDAIRTGASDIHFEPYEKIYRVRLRTDGILHEVARPPIQLATRISAR
LKVMSKMDISERRVPQDGRIKMKLSKTKAIDFRVNSLPTLWGEKIVLRILDPSSAKLGIDALGYEEFQKKIYMDALAQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGVNQVNVSNKVGLNFAEALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPTFNIATSVSVIIAQRLARRLCGECKKPADLPEEILKEEGFD
TVTIPKSEWKIYQPVGCEHCSKGYKGRVGVYEVVRITDGISRIIMEGGNSIQIADQAKKEGFNNLRISALRKVVMGVTSL
EEANRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=899031 R5R33_RS04495 WP_318954850.1 993568..995277(+) (pilB) [Microbulbifer pacificus strain SPO729]
ATGAGCAGCTCCCCTCTCAGTGGCCTGGCCAAACGGCTGGTTCAAGATAAAGCCTTCGATGAGGCAACAGCCCAATCCGC
CATCAAAGCTGCCAAGCGTGAGGGACACAGCTTTGCTCAACATGTAGTAGAAGCTAAGTTGATCAAAAGCCGTGAGCTCG
CCAATATTGCCTCGGTGGCCTTTGGCACCCCCCTGTTTGATCTTTCTAGTTACAATTTTGACCTGCTACCCAAAGGTATA
GTTGATGAAAAACTGATCACAAAGCATTTCGCTCTTCCGCTATATAGACGTGGCAATCGGCTGTTTGTAGCGGTAGCAGA
TCCCACCAATCTGGCGGGGCTGGATGAGATCAATTTCAATACCGGCCTCAATACCGACGCGGTGCTGGTGGAAGCGGACA
AGCTGGCGAAGGCCATTGAAAACTACCTGAACAACAGCGATGTAGGTGGTGGCCTTGAAGGACTGGATGACGAAGATTTA
GACAGCCTGGATGTAGAGTCTGGGGATACCCGCCGCGAGGACGACAATGAACCGGGCGGAGATGAAGCCCCGGTTGTCCG
TTTTGTGAACAAGGTACTATTGGACGCCATCAGAACTGGCGCTTCGGATATTCACTTCGAACCCTACGAGAAAATTTATC
GGGTGCGGTTGCGTACCGACGGCATTCTGCACGAGGTAGCCCGCCCGCCGATTCAGCTGGCCACCCGTATCTCCGCGCGC
CTAAAAGTGATGTCCAAGATGGATATTTCCGAGCGCCGTGTGCCACAGGATGGCCGGATCAAGATGAAGCTGTCCAAGAC
AAAAGCCATCGACTTCCGGGTAAACAGCTTGCCTACTCTCTGGGGGGAGAAGATCGTACTGCGGATTCTGGACCCCTCCT
CTGCCAAACTGGGCATCGACGCCTTGGGTTATGAGGAATTCCAGAAAAAGATCTATATGGATGCCCTGGCACAACCCCAG
GGTATGATTCTGGTCACTGGGCCTACGGGTTCCGGTAAAACAGTATCCCTTTATACCGGCCTGAATATTCTCAATACTCC
GGAGCGCAATATTTCCACTGCGGAAGATCCAGTGGAGATCAACCTAGAGGGTGTCAACCAGGTCAACGTTTCCAATAAGG
TGGGGCTGAACTTTGCGGAGGCCCTGCGCTCCTTCCTGCGGCAAGACCCGGATATCGTTATGGTGGGGGAGATCCGGGAC
CTTGAAACCGCAGAAATCGCGATCAAGGCGGCGCAAACCGGGCACTTGGTGCTGTCTACGCTACACACAAACTCAGCGCC
CGAAACGCTCACTCGCTTGATGAACATGGGGGTACCCACCTTCAATATCGCGACTTCTGTCAGTGTGATTATTGCCCAGC
GTCTGGCCCGACGACTGTGCGGTGAATGTAAAAAGCCTGCAGATCTGCCGGAAGAAATATTGAAAGAGGAAGGTTTCGAT
ACCGTCACCATCCCCAAATCTGAATGGAAGATATACCAGCCGGTGGGTTGTGAACATTGCTCCAAAGGCTACAAAGGCCG
CGTTGGTGTGTATGAAGTGGTTCGCATCACTGACGGAATTTCGCGCATTATAATGGAGGGCGGTAATTCCATTCAGATTG
CTGACCAAGCCAAGAAAGAAGGCTTCAATAACCTGCGTATATCGGCCCTTCGCAAGGTGGTGATGGGCGTTACCAGCTTG
GAAGAAGCCAACCGGGTAACAAAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.646

100

0.619
  pilB Acinetobacter baylyi ADP1

59.541

99.473

0.592
  pilB Legionella pneumophila strain ERS1305867

55.203

99.649

0.55
  pilB Vibrio parahaemolyticus RIMD 2210633

52.66

99.121

0.522
  pilB Vibrio cholerae strain A1552

50.871

100

0.513
  pilB Vibrio campbellii strain DS40M4

51.504

99.297

0.511
  pilF Neisseria gonorrhoeae MS11

48.496

99.297

0.482
  pilB/pilB1 Synechocystis sp. PCC 6803

37.724

100

0.408
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.982

95.782

0.364