Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   R4540_RS09930 Genome accession   NZ_CP137135
Coordinates   2207896..2209122 (+) Length   408 a.a.
NCBI ID   WP_000648511.1    Uniprot ID   Q9X4G9
Organism   Vibrio cholerae V52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2202896..2214122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4540_RS09905 (R4540_09900) fldB 2203019..2203537 (+) 519 WP_000690110.1 flavodoxin FldB -
  R4540_RS09910 (R4540_09905) ampD 2203688..2204233 (-) 546 WP_000567316.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R4540_RS09915 (R4540_09910) nadC 2204529..2205419 (+) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R4540_RS09920 (R4540_09915) pilA 2205664..2206134 (+) 471 WP_000649323.1 type IV pilin protein Machinery gene
  R4540_RS09925 (R4540_09920) pilB 2206165..2207853 (+) 1689 WP_053048901.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R4540_RS09930 (R4540_09925) pilC 2207896..2209122 (+) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  R4540_RS09935 (R4540_09930) pilD 2209180..2210055 (+) 876 WP_000418755.1 A24 family peptidase Machinery gene
  R4540_RS09940 (R4540_09935) coaE 2210052..2210660 (+) 609 WP_000011559.1 dephospho-CoA kinase -
  R4540_RS09945 (R4540_09940) zapD 2210692..2211432 (+) 741 WP_000207192.1 cell division protein ZapD -
  R4540_RS09950 (R4540_09945) yacG 2211590..2211787 (+) 198 WP_000162866.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45134.43 Da        Isoelectric Point: 10.3424

>NTDB_id=896438 R4540_RS09930 WP_000648511.1 2207896..2209122(+) (pilC) [Vibrio cholerae V52]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKSKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTEAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=896438 R4540_RS09930 WP_000648511.1 2207896..2209122(+) (pilC) [Vibrio cholerae V52]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTCTCTTTATTGGCGCGCCTAACCCATCGCGTGAAAAGCAAAGACATCACGATTTTGACTCGGCAGTTAGCGACCATG
CTCACCACGGGCGTACCGATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGCGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGTACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTGGAAACCGGAGAGATGTCCGGTAACTTACCTGAAGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAGCTACGCGCCAAAGTGATTAAAGCGCTCATCTACCCCAGCATGGTTGTGTTGGTCGCGCTCGGGGTATC
TTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTTAAAGGCTTTGGTGCTGAACTGCCTTGGTTTACGC
AGCAAGTGCTGAAACTCTCACACTGGGTACAGGCTTACAGTTTATGGGCATTTATCGCCATCGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCTTTCCAGATCCGTTTAAAAACCAGCCGCTTGGGGCTGAAATTTCCGATTATTGGCAA
TGTACTCGCCAAAGCCTCCATCGCCAAATTCAGCCGCACCCTCGCCACCAGCTTTGCCGCGGGGATCCCGATCCTCGCCA
GTTTAAAAACCACGGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTACATTGCCATGCGCAATACCGAAGCTTTTCCCGAAATGGTGCTGCAAATGGTGATGATTGGTGAAGA
GTCAGGGCAATTAGATGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACGGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTCTTTCTTGGGACGGTTGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9X4G9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

100

100

1
  pilC Vibrio campbellii strain DS40M4

73.75

98.039

0.723
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.294

98.529

0.407
  pilG Neisseria meningitidis 44/76-A

40.796

98.529

0.402