Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   R4540_RS09920 Genome accession   NZ_CP137135
Coordinates   2205664..2206134 (+) Length   156 a.a.
NCBI ID   WP_000649323.1    Uniprot ID   -
Organism   Vibrio cholerae V52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2200664..2211134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4540_RS09895 (R4540_09890) dsbC 2201204..2201956 (-) 753 WP_000115055.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  R4540_RS09900 (R4540_09895) xerD 2201973..2202881 (-) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  R4540_RS09905 (R4540_09900) fldB 2203019..2203537 (+) 519 WP_000690110.1 flavodoxin FldB -
  R4540_RS09910 (R4540_09905) ampD 2203688..2204233 (-) 546 WP_000567316.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R4540_RS09915 (R4540_09910) nadC 2204529..2205419 (+) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R4540_RS09920 (R4540_09915) pilA 2205664..2206134 (+) 471 WP_000649323.1 type IV pilin protein Machinery gene
  R4540_RS09925 (R4540_09920) pilB 2206165..2207853 (+) 1689 WP_053048901.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R4540_RS09930 (R4540_09925) pilC 2207896..2209122 (+) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  R4540_RS09935 (R4540_09930) pilD 2209180..2210055 (+) 876 WP_000418755.1 A24 family peptidase Machinery gene
  R4540_RS09940 (R4540_09935) coaE 2210052..2210660 (+) 609 WP_000011559.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 15828.93 Da        Isoelectric Point: 7.7816

>NTDB_id=896436 R4540_RS09920 WP_000649323.1 2205664..2206134(+) (pilA) [Vibrio cholerae V52]
MKAYKNKLQQGFTLIELMIVVAVIGVLAAIAVPQYQNYVKKSEAAAAVATIRSLTTNIDTFMADTGNFPTDLTTLGAASN
MNKLGTITLGTNEVKLVFSNTDSAFASTDEVKMAKDATSGLWTCTVPTGVTLKGCTASGSGSGSGSGSGSGSGSGN

Nucleotide


Download         Length: 471 bp        

>NTDB_id=896436 R4540_RS09920 WP_000649323.1 2205664..2206134(+) (pilA) [Vibrio cholerae V52]
ATGAAAGCGTATAAAAACAAACTACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTAGCAGTGATTGGTGTATT
GGCAGCGATTGCGGTTCCTCAATATCAAAACTACGTTAAAAAATCAGAAGCTGCAGCTGCAGTAGCAACAATACGCTCTT
TGACTACCAATATCGATACATTCATGGCTGATACCGGAAATTTTCCAACTGATTTAACTACATTGGGTGCAGCATCAAAT
ATGAATAAACTCGGTACGATTACACTTGGGACAAACGAAGTTAAATTAGTTTTCAGTAATACTGACTCCGCTTTTGCTTC
AACTGATGAAGTTAAAATGGCTAAAGATGCAACTTCTGGCCTTTGGACTTGTACTGTACCAACAGGTGTAACACTGAAAG
GATGTACAGCTTCTGGTTCTGGTTCTGGTTCTGGTTCTGGTTCTGGTTCTGGTTCTGGTTCTGGCAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-131)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50

97.436

0.487
  pilA Vibrio cholerae strain A1552

50

97.436

0.487
  pilA Vibrio cholerae C6706

50

97.436

0.487
  pilA Vibrio parahaemolyticus RIMD 2210633

42.657

91.667

0.391
  pilA Pseudomonas aeruginosa PAK

42.963

86.538

0.372
  pilA Acinetobacter baumannii strain A118

39.86

91.667

0.365