Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R3L12_RS14300 Genome accession   NZ_CP137057
Coordinates   3050675..3052390 (-) Length   571 a.a.
NCBI ID   WP_149929792.1    Uniprot ID   -
Organism   Acinetobacter soli strain SNUBHAB0075     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3045675..3057390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3L12_RS14270 (R3L12_14250) - 3045846..3046172 (+) 327 WP_004932453.1 pyrimidine/purine nucleoside phosphorylase -
  R3L12_RS14275 (R3L12_14255) rlmB 3046274..3047020 (+) 747 WP_004932455.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  R3L12_RS14280 (R3L12_14260) - 3047058..3047987 (+) 930 WP_025095298.1 DMT family transporter -
  R3L12_RS14285 (R3L12_14265) coaE 3047963..3048562 (-) 600 WP_149929795.1 dephospho-CoA kinase -
  R3L12_RS14290 (R3L12_14270) pilD 3048565..3049425 (-) 861 WP_149929794.1 A24 family peptidase Machinery gene
  R3L12_RS14295 (R3L12_14275) pilC 3049425..3050651 (-) 1227 WP_149929793.1 type II secretion system F family protein Machinery gene
  R3L12_RS14300 (R3L12_14280) pilB 3050675..3052390 (-) 1716 WP_149929792.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R3L12_RS14305 (R3L12_14285) tpiA 3052654..3053451 (+) 798 WP_004932474.1 triose-phosphate isomerase -
  R3L12_RS14310 (R3L12_14290) secG 3053461..3053790 (+) 330 WP_004932477.1 preprotein translocase subunit SecG -
  R3L12_RS14330 (R3L12_14310) rimP 3054867..3055391 (+) 525 WP_004932478.1 ribosome maturation factor RimP -
  R3L12_RS14335 (R3L12_14315) nusA 3055427..3056911 (+) 1485 WP_004943981.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 64220.87 Da        Isoelectric Point: 5.1770

>NTDB_id=894490 R3L12_RS14300 WP_149929792.1 3050675..3052390(-) (pilB) [Acinetobacter soli strain SNUBHAB0075]
MSLVTASPKFSGFIRRLVEEGHIDAHDMQMAQESAKKNKQDIVPYLIDHYAITPITIAETISVEFGEPLFDLDVFDPALI
LKDRIDEKLIRKYRVMPLVHRGHVLYVATCNPTNLEAMDAIRFNSKLKVEPIIVEQDKLERLLNEYFPEDSRFNFDTEDF
DLDLEVNTPTSDDDDDEEISKDEAPVVKYINKLLIDAIRMNASDLHFEPYEKMYRVRYRVDGVLRLIATPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKTIDFRVNSLPTLFGEKIVLRILDPASAMLGIDALGYEPEQKALFTEALNRP
QGMLLITGPTGSGKTVSMYTGLNILNTEHANISTAEDPVEINLEGINQVNVNPKVGLTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHLVMSTLHTNNAAETLTRLRSMGVATFNIATSVNLVIAQRLARRLCNQCKRRIDVPRASLLEMGF
TETDLDAPDFHIYEPVGCKECREGYKGRVGIYEVMKITPEISRIIMQDGNALEIAAAAEQLGFSNLRRSGLKKVMQGVTS
LQEVNRVTNEE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=894490 R3L12_RS14300 WP_149929792.1 3050675..3052390(-) (pilB) [Acinetobacter soli strain SNUBHAB0075]
ATGTCATTAGTTACCGCATCACCTAAATTTTCAGGATTCATTCGGCGCCTTGTCGAAGAAGGTCATATTGATGCACACGA
CATGCAAATGGCTCAAGAGAGCGCCAAAAAAAACAAGCAAGATATTGTTCCTTATTTGATCGACCATTATGCCATTACCC
CGATCACCATTGCTGAAACCATCTCGGTCGAGTTTGGCGAACCGCTATTTGATCTGGATGTTTTTGATCCTGCGCTGATT
CTCAAAGATCGTATCGATGAAAAGCTGATTCGCAAGTATCGTGTAATGCCGTTGGTTCATCGTGGGCATGTGCTCTATGT
CGCGACCTGCAATCCGACCAATCTTGAAGCGATGGATGCCATCCGTTTTAACAGCAAACTCAAAGTCGAACCGATTATTG
TAGAACAGGATAAGCTTGAGCGACTGTTAAACGAATATTTCCCCGAAGATAGCCGTTTTAACTTTGACACCGAAGATTTT
GATCTGGATCTGGAAGTGAATACCCCCACTTCAGATGACGACGACGATGAAGAAATCAGTAAAGATGAAGCCCCCGTGGT
CAAATACATCAATAAATTACTGATTGATGCCATTCGTATGAATGCATCAGACTTACATTTTGAACCTTATGAAAAAATGT
ATCGGGTCCGTTATCGGGTCGATGGTGTACTTCGTTTAATTGCAACACCACCCTTACAACTGGCTACCCGACTGGCCTCG
CGTTTGAAAGTGATGTCTCAAATGGATATTTCGGAAAAGCGTGTTCCGCAAGATGGTCGTATTAAACTGAAACTGTCGAA
AACCAAAACCATCGATTTTCGGGTCAACTCCCTACCGACCCTATTTGGTGAAAAGATTGTACTGCGGATTCTGGATCCTG
CGAGCGCAATGCTGGGTATCGATGCACTGGGCTACGAGCCTGAGCAAAAAGCATTGTTTACCGAAGCGCTGAATCGACCA
CAAGGCATGCTGCTGATTACCGGCCCTACAGGCTCGGGGAAAACCGTATCGATGTATACTGGCTTAAATATTCTCAATAC
CGAACATGCCAATATTTCTACCGCCGAAGACCCAGTCGAGATTAATCTTGAAGGTATTAATCAGGTCAATGTAAATCCCA
AAGTCGGCCTGACCTTTGCTGCGGCATTACGCTCTTTTTTACGTCAAGACCCTGATATCATTATGGTGGGGGAAATTCGT
GATTTAGAAACCGCAGAGATTGCCATTAAAGCGGCTCAAACCGGTCACTTGGTGATGTCGACACTGCATACCAATAACGC
TGCCGAAACACTTACACGCCTGAGAAGCATGGGGGTGGCGACCTTTAATATCGCCACCTCAGTCAATCTGGTGATTGCGC
AGCGCCTTGCCAGACGCCTGTGTAATCAGTGTAAACGGCGTATCGACGTGCCACGTGCCAGTTTACTCGAAATGGGATTT
ACCGAAACCGATTTGGATGCTCCAGACTTTCATATCTATGAACCGGTGGGGTGTAAAGAATGTCGGGAGGGCTACAAAGG
ACGTGTGGGCATTTATGAAGTCATGAAAATTACACCGGAAATCTCAAGAATTATTATGCAAGACGGCAATGCACTTGAAA
TTGCAGCAGCCGCTGAACAGCTTGGATTTAGCAACTTACGCCGTTCTGGCTTAAAAAAAGTGATGCAGGGGGTTACATCG
TTACAGGAAGTCAATCGTGTGACGAATGAAGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

86.14

99.825

0.86
  pilB Acinetobacter baumannii D1279779

77.544

99.825

0.774
  pilB Legionella pneumophila strain ERS1305867

51.304

100

0.517
  pilF Neisseria gonorrhoeae MS11

50

98.424

0.492
  pilB Vibrio cholerae strain A1552

51.644

90.543

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

50.769

91.068

0.462
  pilB Vibrio campbellii strain DS40M4

50.097

90.543

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

35.668

100

0.384
  pilF Thermus thermophilus HB27

37.77

97.373

0.368