Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R2X36_RS19015 Genome accession   NZ_CP137009
Coordinates   4104484..4106190 (+) Length   568 a.a.
NCBI ID   WP_218410023.1    Uniprot ID   -
Organism   Aeromonas media strain QST31     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4099484..4111190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2X36_RS18990 (R2X36_18990) pdhR 4100636..4101400 (-) 765 WP_005329211.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  R2X36_RS18995 (R2X36_18995) ampD 4101678..4102250 (-) 573 WP_317841573.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R2X36_RS19000 (R2X36_19000) - 4102396..4102857 (+) 462 WP_374393893.1 TIGR02281 family clan AA aspartic protease -
  R2X36_RS19005 (R2X36_19005) nadC 4102861..4103724 (+) 864 WP_317841574.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R2X36_RS19010 (R2X36_19010) - 4104037..4104480 (+) 444 WP_317841575.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  R2X36_RS19015 (R2X36_19015) pilB 4104484..4106190 (+) 1707 WP_218410023.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  R2X36_RS19020 (R2X36_19020) pilC 4106320..4107561 (+) 1242 WP_043133310.1 type II secretion system F family protein Machinery gene
  R2X36_RS19025 (R2X36_19025) pilD 4107642..4108514 (+) 873 WP_042649027.1 A24 family peptidase Machinery gene
  R2X36_RS19030 (R2X36_19030) coaE 4108533..4109147 (+) 615 WP_317841576.1 dephospho-CoA kinase -
  R2X36_RS19035 (R2X36_19035) zapD 4109185..4109907 (+) 723 WP_226170498.1 cell division protein ZapD -
  R2X36_RS19040 (R2X36_19040) yacG 4109917..4110111 (+) 195 WP_042649030.1 DNA gyrase inhibitor YacG -
  R2X36_RS19045 (R2X36_19045) mutT 4110187..4110591 (-) 405 WP_005332118.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62669.08 Da        Isoelectric Point: 5.8224

>NTDB_id=894229 R2X36_RS19015 WP_218410023.1 4104484..4106190(+) (pilB) [Aeromonas media strain QST31]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLSEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEEGKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDDSVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKVVIRLLDSSVSRLNLDQLGFDERQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKVPEAVPEAELLEFGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=894229 R2X36_RS19015 WP_218410023.1 4104484..4106190(+) (pilB) [Aeromonas media strain QST31]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCCTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCAAGGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATCGAAAACGACATCCTCGACAGCAAGGCTCTCG
CAGACTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTTGACCTCTCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAATTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACTCTCTATATCGCCATGTCAGA
CCCGACCAATGTGTCGGCCCTGGAGGACTTTGGCTTCAGCTTCGGCCTGCATACCGAAGCCTTGCTGGTCGAAGAGGGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCAGGACACTCTGGGCATGGATCACATCGACGAAGCCGAAATC
TCGGAACTCGAGGTCTCCGATGAGAGTTCGCGGCTCGACGACAGCGTCAACACTGCCGACGACGATGCCCCCATCGTCAA
ATACATTCACAAGATCATGATGGACGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAACCCTATGAGACCAAGTACC
GGATCCGCTTCCGGATCGACGGCATACTGCACGAAATCGCCACCCCGCCGGTCAATCTGGCGAACCGTTTCGCGGCTCGC
TTAAAGGTAATGGCGCGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCCCGCAA
CCGGTCCATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAAAAGGTCGTCATTCGCCTGCTGGACTCGTCAG
TATCTCGCCTCAACCTAGACCAACTCGGCTTCGACGAACGCCAGAAGGCGCAATATCTGCATGCGCTCTCCAAGCCACAG
GGCATGATACTGGTCACGGGCCCCACGGGATCGGGCAAGACAGTATCACTCTATACCGGTCTCAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGATCCAGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTCCTGCGCCAGGATCCTGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCTCAGACCGGACACCTGGTGCTGTCAACCCTGCACACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GCCTCGCACGCAAGCTGTGCGATCACTGCAAGGTGCCCGAGGCAGTGCCGGAAGCCGAATTGCTGGAGTTCGGTTTTACC
CAGCAACAGCTGGCCGCCGGTTTGCGGCTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCGGGAGGATACAGAGGACGGGT
CGGCATCTATGAAATCATGCTGATGTCGGAGAACATCGCCAAGCTGATCATGCAGGGAGCAAACTCGCTGCAGATCGCTG
CCATCGCCCAGAAGGAGGGTATGCGTACCCTGCGCATTTCCGGCCTGGAGAAGGCCCGTCTGGGGGTCACCAGCCTGGCC
GAGATCAACAGGATCACGACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.85

99.824

0.548
  pilB Acinetobacter baylyi ADP1

54.529

99.12

0.54
  pilB Legionella pneumophila strain ERS1305867

53.901

99.296

0.535
  pilB Vibrio cholerae strain A1552

52.837

99.296

0.525
  pilB Vibrio parahaemolyticus RIMD 2210633

49.378

99.12

0.489
  pilB Vibrio campbellii strain DS40M4

49.378

99.12

0.489
  pilF Neisseria gonorrhoeae MS11

46.032

99.824

0.46
  pilF Thermus thermophilus HB27

39.736

93.486

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

38.043

97.183

0.37