Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R0135_RS07180 Genome accession   NZ_CP136864
Coordinates   1627493..1629214 (+) Length   573 a.a.
NCBI ID   WP_407349572.1    Uniprot ID   -
Organism   Congregibacter variabilis strain IMCC43200     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1622493..1634214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R0135_RS07165 (R0135_07135) - 1623554..1624423 (-) 870 WP_407349570.1 glycosyltransferase family 2 protein -
  R0135_RS07170 (R0135_07140) - 1624514..1626523 (-) 2010 WP_407349571.1 hypothetical protein -
  R0135_RS07175 (R0135_07145) comP 1626649..1627110 (-) 462 WP_407349887.1 pilin Machinery gene
  R0135_RS07180 (R0135_07150) pilB 1627493..1629214 (+) 1722 WP_407349572.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R0135_RS07185 (R0135_07155) pilC 1629271..1630488 (+) 1218 WP_407349573.1 type II secretion system F family protein Machinery gene
  R0135_RS07190 (R0135_07160) pilD 1630532..1631419 (+) 888 WP_407349574.1 prepilin peptidase Machinery gene
  R0135_RS07195 (R0135_07165) coaE 1631416..1632024 (+) 609 WP_407349575.1 dephospho-CoA kinase -
  R0135_RS07200 (R0135_07170) mutT 1632080..1632511 (-) 432 WP_407349576.1 8-oxo-dGTP diphosphatase MutT -
  R0135_RS07205 (R0135_07175) argJ 1632520..1633758 (-) 1239 WP_407349577.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62915.51 Da        Isoelectric Point: 4.7576

>NTDB_id=893153 R0135_RS07180 WP_407349572.1 1627493..1629214(+) (pilB) [Congregibacter variabilis strain IMCC43200]
MNERVSGKPSGLAGRLVIEGLLDEDQASKAQAAATSERIPFVRYLVDEVKIDSHKLAEIASQEFGAARFDIGAFDSQFLP
QGVVEPELVTKHHALPLYSRGKRLFIAVSDPTNLAALDEIKFHTGLNTEAILVDEKSLQKAIAEWTDAQDDMSGGLDDLE
SDEFDEIDIGAVDNDAAPEDDGNSSVDETPIVRFVNKVLVDAIKQGASDIHFEPYEKDYRVRFRTDGVLREVVRPPRNLA
PRLSARLKVMSQMDISERRMPQDGRIQMKLSRNRSIDFRVNTLPTLFGEKIVLRILDPTSAQMGIDALGYDPDQKDMFMK
ALKQPQGMILVTGPTGSGKTVSLYTGLNILNEPERNISTAEDPVEINLSGINQVHVNAKVGLNFAEALRSFLRQDPDVIM
VGEIRDLETAEIAIKAAQTGHLVLSTLHTNSAAETISRLLNMGVPAFNVATSVSLIIAQRLGRRLCKECSAPAEIPHDTL
LEQGFTEEMLTSATILKAVGCDACKDGYKGRVGIYEVVRITPEIANTIMEDGNSLEIARQARTHGFADLRTSALRKCAQG
LISLEEVNRVTID

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=893153 R0135_RS07180 WP_407349572.1 1627493..1629214(+) (pilB) [Congregibacter variabilis strain IMCC43200]
ATGAACGAACGAGTCAGCGGTAAACCCTCCGGCCTAGCCGGTCGCCTTGTTATCGAGGGCCTCCTCGATGAAGATCAGGC
GTCCAAGGCTCAGGCCGCCGCAACCAGTGAACGAATCCCTTTCGTTCGGTATTTGGTCGATGAAGTAAAAATCGACAGCC
ACAAGCTGGCTGAAATTGCTTCACAGGAATTCGGCGCAGCGCGATTCGACATAGGGGCTTTTGATTCTCAGTTTTTGCCT
CAGGGAGTTGTAGAGCCCGAACTGGTCACCAAACATCACGCGCTGCCGCTCTACAGTCGGGGCAAGCGCCTCTTCATAGC
AGTCTCCGATCCCACCAACCTTGCAGCGCTTGATGAGATCAAATTTCACACAGGTCTGAATACCGAAGCCATTTTGGTTG
ATGAGAAGAGTCTACAAAAGGCTATTGCTGAATGGACCGATGCCCAGGACGACATGAGCGGCGGTCTCGACGATCTTGAG
TCCGATGAGTTTGATGAAATTGATATTGGCGCAGTCGATAATGATGCAGCCCCGGAGGACGACGGTAACAGCAGTGTTGA
CGAGACTCCGATTGTACGATTTGTCAACAAGGTGCTGGTCGATGCCATCAAACAGGGCGCTTCGGACATTCACTTTGAGC
CTTATGAAAAAGACTACCGCGTTCGTTTTCGGACAGACGGCGTACTCAGAGAAGTTGTCCGTCCACCTCGTAATTTGGCA
CCTCGTCTGTCGGCTCGACTGAAAGTTATGTCGCAAATGGATATTTCTGAGCGACGAATGCCTCAGGACGGTCGCATTCA
AATGAAGCTCTCGCGAAATCGATCTATTGATTTTCGAGTCAACACACTGCCTACGCTTTTTGGCGAGAAGATAGTGCTGC
GGATTTTGGATCCCACTAGCGCCCAAATGGGTATCGATGCCCTCGGCTACGATCCGGATCAGAAAGACATGTTTATGAAG
GCGCTGAAACAGCCTCAGGGCATGATCCTGGTCACAGGGCCAACGGGTTCCGGCAAGACGGTTTCACTATATACAGGTCT
CAATATCCTTAATGAGCCCGAGCGAAATATCTCCACTGCGGAAGACCCTGTAGAAATCAATCTCTCGGGCATCAACCAGG
TGCACGTGAACGCCAAGGTCGGTCTCAACTTTGCCGAGGCCTTACGCTCCTTCCTGCGCCAGGATCCAGACGTCATCATG
GTCGGCGAGATACGTGACCTTGAAACAGCAGAAATCGCTATTAAAGCGGCGCAAACCGGCCACCTTGTGCTGTCAACGCT
TCACACCAACAGCGCCGCAGAAACTATCAGCCGTCTACTCAACATGGGAGTACCCGCGTTTAACGTCGCGACCTCTGTGA
GTCTTATTATTGCCCAGCGTCTGGGCCGTCGGCTTTGTAAAGAGTGTTCGGCTCCGGCAGAAATACCTCACGACACCTTG
CTTGAACAGGGATTTACTGAGGAAATGCTCACTTCAGCTACGATTTTAAAGGCCGTGGGCTGCGATGCGTGTAAGGATGG
CTATAAAGGTCGGGTCGGTATTTACGAAGTCGTTCGCATAACGCCGGAAATCGCCAACACTATTATGGAAGACGGAAACT
CTCTGGAGATTGCCCGTCAGGCGAGAACGCACGGCTTTGCAGACCTCCGTACATCGGCGTTACGAAAATGTGCCCAAGGA
CTGATTAGTCTTGAAGAAGTAAACCGAGTTACGATTGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.172

99.302

0.578
  pilB Acinetobacter baylyi ADP1

57.597

98.778

0.569
  pilB Legionella pneumophila strain ERS1305867

53.71

98.778

0.531
  pilB Vibrio parahaemolyticus RIMD 2210633

51.499

98.953

0.51
  pilB Vibrio cholerae strain A1552

52.087

96.161

0.501
  pilB Vibrio campbellii strain DS40M4

52.49

91.099

0.478
  pilF Neisseria gonorrhoeae MS11

48.479

97.557

0.473
  pilB/pilB1 Synechocystis sp. PCC 6803

41.468

87.958

0.365