Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RZV13_RS14750 Genome accession   NZ_CP136579
Coordinates   3449355..3450485 (-) Length   376 a.a.
NCBI ID   WP_005990431.1    Uniprot ID   Q8P759
Organism   Xanthomonas cannabis strain DAR41374     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3444355..3455485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZV13_RS14720 (RZV13_14670) - 3444565..3445512 (-) 948 WP_039404804.1 aspartate carbamoyltransferase catalytic subunit -
  RZV13_RS14725 (RZV13_14675) ruvX 3445526..3445993 (-) 468 WP_228334037.1 Holliday junction resolvase RuvX -
  RZV13_RS14730 (RZV13_14680) - 3445986..3446552 (-) 567 WP_039404799.1 YqgE/AlgH family protein -
  RZV13_RS14735 (RZV13_14685) - 3446858..3447424 (+) 567 WP_228334036.1 DNA-3-methyladenine glycosylase I -
  RZV13_RS14740 (RZV13_14690) - 3447461..3448360 (+) 900 WP_228314138.1 DUF72 domain-containing protein -
  RZV13_RS14745 (RZV13_14695) - 3448373..3449047 (-) 675 WP_039566430.1 YitT family protein -
  RZV13_RS14750 (RZV13_14700) pilU 3449355..3450485 (-) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RZV13_RS14755 (RZV13_14705) pilT 3450599..3451636 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  RZV13_RS14760 (RZV13_14710) - 3451964..3452656 (+) 693 WP_355581652.1 YggS family pyridoxal phosphate-dependent enzyme -
  RZV13_RS14765 (RZV13_14715) proC 3452700..3453554 (+) 855 WP_355581654.1 pyrroline-5-carboxylate reductase -
  RZV13_RS14770 (RZV13_14720) - 3454111..3454533 (+) 423 WP_047693787.1 HU family DNA-binding protein -
  RZV13_RS14775 (RZV13_14725) - 3454659..3455267 (+) 609 WP_355581657.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41996.26 Da        Isoelectric Point: 6.8287

>NTDB_id=891681 RZV13_RS14750 WP_005990431.1 3449355..3450485(-) (pilU) [Xanthomonas cannabis strain DAR41374]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=891681 RZV13_RS14750 WP_005990431.1 3449355..3450485(-) (pilU) [Xanthomonas cannabis strain DAR41374]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCCGGGAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACGCAGACACCGTTGACCGCGCAGCAAAGCCGCGACCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAGTTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTCCGTGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CACCGTGGAAGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTGGGCATTGATACCGACAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGATCACGCTATCGCTTTCGCCGAAACCGGCCACCTGGTGCTGTGTACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGGCGCAACCAGCTGTTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGATGGTCGCGGCCGGCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGTCTGTTCGAGCTCTACCAGGCCGGCGAGATCAGCTACGAAGACGCCTTGCGCTACGCCGATTCGCAAAACGA
GGTGCGTTTGCGCATCAAGCTGTCGCAGGGCGGCGATGCCAAGACCTTGTCGCAGGGCCTGGATGGCGTGGAGATTGCCG
AAGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8P759

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367