Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RZV16_RS16170 Genome accession   NZ_CP136578
Coordinates   3794852..3796600 (-) Length   582 a.a.
NCBI ID   WP_355505404.1    Uniprot ID   -
Organism   Xanthomonas cannabis strain DAR82727     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3789852..3801600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZV16_RS16155 (RZV16_16100) - 3790488..3792101 (+) 1614 WP_039405515.1 HAMP domain-containing sensor histidine kinase -
  RZV16_RS16160 (RZV16_16105) pilR 3792626..3794020 (+) 1395 WP_355505398.1 sigma-54 dependent transcriptional regulator Regulator
  RZV16_RS16165 (RZV16_16110) - 3794668..3794787 (-) 120 WP_355505401.1 pilus assembly protein -
  RZV16_RS16170 (RZV16_16115) pilB 3794852..3796600 (-) 1749 WP_355505404.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RZV16_RS16175 (RZV16_16120) pilA 3796722..3797150 (-) 429 WP_355505406.1 pilin Machinery gene
  RZV16_RS16180 (RZV16_16125) pilC 3797489..3798751 (+) 1263 WP_355505409.1 type II secretion system F family protein Machinery gene
  RZV16_RS16185 (RZV16_16130) - 3798758..3799621 (+) 864 WP_046343941.1 A24 family peptidase -
  RZV16_RS16190 (RZV16_16135) coaE 3799635..3800258 (+) 624 WP_355505413.1 dephospho-CoA kinase -
  RZV16_RS16195 (RZV16_16140) - 3800330..3800508 (+) 179 Protein_3170 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63222.46 Da        Isoelectric Point: 5.6073

>NTDB_id=891670 RZV16_RS16170 WP_355505404.1 3794852..3796600(-) (pilB) [Xanthomonas cannabis strain DAR82727]
MEKIVTSAVAANLVGITGIARRLVQDGALDEISARNAMDQAAAAKTPLPQWFSERKLVSASQLAAANAVEFGMPLMDVSV
FDASQNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALG
SSLGNDDDDIGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNV
AKAPVKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEA
DQQKLFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFL
RQDPDIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNHCKRK
SSLPENALLAEGFTPEQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQ
SALMKAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=891670 RZV16_RS16170 WP_355505404.1 3794852..3796600(-) (pilB) [Xanthomonas cannabis strain DAR82727]
ATGGAGAAGATTGTGACGAGTGCAGTTGCGGCGAACTTAGTAGGAATTACAGGCATTGCACGCCGCTTGGTTCAAGACGG
AGCACTGGACGAAATAAGTGCCCGTAACGCCATGGACCAAGCTGCAGCAGCGAAAACTCCGCTTCCCCAATGGTTCTCCG
AGAGAAAGCTGGTTTCAGCGTCTCAACTTGCCGCCGCCAATGCGGTCGAGTTCGGCATGCCGTTAATGGATGTGTCGGTG
TTTGACGCCAGCCAGAACGCGGTCAAGCTGGTCAGTGAAGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCG
CGGCAACCGGTTGTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGATGACATCAAGTTCCATACCAATTTGG
TGGTCGAGCCGATTCTGGTCGACGAGGACCAGATCCGTCGCACCCTGGAACAGTGGCAGGCCAGTAATGCCGCGCTTGGG
TCGTCGCTAGGTAATGACGATGACGATATAGGCGACCTGGACGTGTCGGCTGGCGACGAGGACATGGGAGCCGGCGGGGA
TTCCGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATTCGGCGGG
GAGCCTCTGACATCCATTTCGAGCCATATGAGGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTATTGAAGAACGTG
GCCAAGGCGCCGGTGAAGCTGAACCAGCGCATCGCGGCGCGTTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCG
GGTGCCGCAGGACGGACGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGTACCTTGCCGACCT
TGTTTGGCGAGAAGGTGGTGTTGCGTATCCTGGATGCCAGTGCGGCCAAGCTGGGTATCGAGAAGTTGGGCTACGAGGCG
GATCAGCAAAAGCTGTTCCTGGATGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAA
GACGGTGTCGTTGTATACCGCGCTGGGCATCCTCAATGATGAGACCCGTAATATTTCCACGGCTGAGGATCCGGTCGAAA
TCCGCCTGCCTGGCGTCAATCAGGTGCAGCAGAACGTCAAGCGCGGGATGACGTTTGCTGCGGCGCTGCGCTCGTTCCTG
CGTCAGGATCCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGAC
TGGTCACATGGTGCTGTCCACGTTGCACACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGGATTGCGC
CGTACAACATCACCTCGTCGGTGACCCTGGTCATTGCGCAGCGCCTGGCGCGGCGGTTGTGCAACCACTGCAAGCGCAAG
TCGTCGCTGCCTGAAAATGCGTTGCTTGCTGAAGGTTTTACGCCAGAGCAGATCGCTGCCGGGATCGAACTGTATGAGGC
GGTGGGCTGCGACGAATGCACCGAAGGCTACAAGGGGCGCACCGGTATCTACCAGGTGATGCCGATGACCGATGAGATTG
GCGCCATCGTGCTGGAAGGCGGCAACGCGATGCAGATCGCCGAGGCCGCGCAGAAGATCGGTATCCGCGATCTGCGGCAG
TCGGCGTTGATGAAGGCTGCGCACGGGGTGACCAGCCTGGCTGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-157)

Type II/IV secretion system protein (T2SSE)

(205-472)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

97.595

0.546
  pilB Acinetobacter baylyi ADP1

55.458

97.595

0.541
  pilB Legionella pneumophila strain ERS1305867

51.993

99.141

0.515
  pilF Neisseria gonorrhoeae MS11

48.768

97.595

0.476
  pilB Vibrio cholerae strain A1552

50.091

93.986

0.471
  pilB Vibrio campbellii strain DS40M4

45.694

97.766

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

46.071

96.22

0.443
  pilB/pilB1 Synechocystis sp. PCC 6803

35.516

100

0.373