Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RGE70_RS01155 Genome accession   NZ_CP136522
Coordinates   257175..258881 (-) Length   568 a.a.
NCBI ID   WP_310469723.1    Uniprot ID   -
Organism   Shewanella sp. DAU334     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 252175..263881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGE70_RS01125 (RGE70_01125) mutT 252764..253156 (-) 393 WP_310469717.1 8-oxo-dGTP diphosphatase MutT -
  RGE70_RS01130 (RGE70_01130) yacG 253153..253392 (-) 240 WP_310469718.1 DNA gyrase inhibitor YacG -
  RGE70_RS01135 (RGE70_01135) zapD 253441..254175 (-) 735 WP_310469719.1 cell division protein ZapD -
  RGE70_RS01140 (RGE70_01140) coaE 254184..254789 (-) 606 WP_310469720.1 dephospho-CoA kinase -
  RGE70_RS01145 (RGE70_01145) pilD 254881..255795 (-) 915 WP_310469721.1 A24 family peptidase Machinery gene
  RGE70_RS01150 (RGE70_01150) pilC 255857..257128 (-) 1272 WP_310469722.1 type II secretion system F family protein Machinery gene
  RGE70_RS01155 (RGE70_01155) pilB 257175..258881 (-) 1707 WP_310469723.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RGE70_RS01160 - 259290..259392 (-) 103 Protein_228 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RGE70_RS01165 (RGE70_01165) nadC 260394..261278 (-) 885 WP_310469724.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RGE70_RS01170 (RGE70_01170) ampD 261496..262038 (+) 543 WP_310469725.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RGE70_RS01175 (RGE70_01175) ampE 262083..262934 (+) 852 WP_310469726.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63038.49 Da        Isoelectric Point: 5.7616

>NTDB_id=891324 RGE70_RS01155 WP_310469723.1 257175..258881(-) (pilB) [Shewanella sp. DAU334]
MPSTGLHLGLSTLFTRKKLLTEQQITAAIAKSRKSKQSLVTTVIADQLISAKKIAELCYEEYGTPLLDINEFDLSSIPEE
YLNKKLIEKHQCLPLYRRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEDDKLIAALEKVLEEDISALELDGMDDEA
LANIEVSDTDKRDDELGDGKDDAPIVTYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILKEVSEPPVNLSNRISAR
LKVMAKLDIAERRVPQDGRIKMKMSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRLLYEKMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLSRRLCQACKTPEVIHPDELLRLGFS
VQQIEQGVTPYKPVGCEHCTGGYKGRVGIYEVMQMSDEIARTIMEGGNSLQILKQAKAQGMRDLRDSGLLKVIQGVTSIT
EINRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=891324 RGE70_RS01155 WP_310469723.1 257175..258881(-) (pilB) [Shewanella sp. DAU334]
ATGCCATCCACCGGCCTTCACCTTGGCTTATCCACGCTCTTTACCCGCAAAAAACTGTTAACTGAGCAACAGATTACCGC
TGCCATCGCTAAATCTCGTAAGTCTAAACAATCGCTAGTCACCACAGTTATAGCTGACCAGCTTATTTCCGCCAAAAAAA
TTGCCGAGCTGTGCTACGAAGAATATGGCACCCCTTTACTTGATATTAATGAGTTTGACTTAAGTTCAATCCCCGAAGAG
TATCTAAATAAAAAGCTGATAGAAAAGCACCAATGCTTGCCGCTATATAGACGCGGTAACCGCTTGTATATTGCCACGTC
TGACCCCACCAATATTGCCGCACTAGAGGATTTTCAGTTTAGTGCTGGCCTGCACGCCGAAGCTATTTTAGTCGAAGATG
ACAAGCTGATTGCTGCACTAGAAAAAGTGCTAGAAGAAGATATCAGCGCGCTTGAGCTTGATGGCATGGACGACGAAGCA
CTGGCCAATATTGAAGTTAGCGATACCGATAAACGCGATGACGAACTTGGTGACGGCAAAGACGACGCGCCCATTGTTAC
CTATATCAATAAAATTTTAACCGATGCGATCCGTAAAGGCGCCTCGGACTTACACTTTGAGCCCTATGAAAAACGCTACC
GTATCCGTTTTAGAATTGACGGAATTTTAAAAGAAGTATCGGAGCCACCGGTTAACTTGTCTAACCGTATTTCAGCCCGC
TTAAAAGTAATGGCAAAACTCGACATTGCAGAGCGCCGTGTGCCACAAGATGGCCGCATTAAAATGAAAATGTCGCGCAC
CAAATCTATCGATTTTCGTGTTAGTACGCTGCCAACCATTTGGGGCGAAAAAATTGTAATGCGGATCTTGGACTCCTCTT
CGGCACAATTGGGCATTGAAAAGCTCGGTTACGAAGACGACCAACGGCTTCTGTATGAAAAAATGCTGGCAAAACCGCAA
GGGATGATCCTGGTTACCGGACCTACGGGGTCGGGTAAAACGGTATCGCTTTATACAGGGTTGAACATTCTTAATACCGA
AGAACGTAATATTTCGACTGCCGAAGACCCAGTAGAAATTAACCTTGAAGGGGTCAATCAGGTACACATTAATTTAAAAG
CCGGATTAACCTTTGCCTCGGCCTTGCGATCTTTTTTACGCCAAGATCCCGATGTGGTGATGGTGGGTGAGATCCGCGAT
CTCGAAACCGCTGAAATTGCCATAAAAGCAGCGCAAACTGGTCACTTAGTGTTATCAACACTGCATACTAACTCTGCTGC
CGAAACACTGACTCGCTTGATTAATATGGGCGTTCCCGGGTATAACATTGCCAGCTCGGTTAATTTAATTATTGCCCAGC
GTCTTTCAAGACGACTATGCCAAGCCTGTAAAACGCCTGAAGTTATTCACCCTGATGAACTGCTAAGGCTTGGGTTTAGT
GTGCAACAAATTGAACAAGGTGTTACCCCATATAAGCCGGTTGGCTGCGAACATTGCACTGGCGGCTATAAAGGCCGAGT
CGGAATTTACGAAGTGATGCAGATGTCAGATGAAATAGCGCGCACCATTATGGAAGGTGGTAATTCACTGCAAATACTGA
AACAAGCTAAAGCGCAAGGCATGCGTGATCTGCGTGATTCTGGCTTGTTAAAGGTCATTCAAGGGGTGACCAGTATTACC
GAAATTAACCGCGTTACTAGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.307

96.479

0.572
  pilB Legionella pneumophila strain ERS1305867

56.306

99.12

0.558
  pilB Acinetobacter baumannii D1279779

56.631

98.239

0.556
  pilB Vibrio cholerae strain A1552

51.957

98.944

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

51.15

99.472

0.509
  pilB Vibrio campbellii strain DS40M4

49.912

99.824

0.498
  pilF Neisseria gonorrhoeae MS11

47.703

99.648

0.475
  pilF Thermus thermophilus HB27

38.574

96.303

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.879

99.296

0.366
  pilB/pilB1 Synechocystis sp. PCC 6803

41.118

88.204

0.363