Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RTE98_RS09830 Genome accession   NZ_CP136501
Coordinates   2104274..2105977 (+) Length   567 a.a.
NCBI ID   WP_267932281.1    Uniprot ID   A0AA47E4X7
Organism   Stutzerimonas frequens strain L27     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2099274..2110977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RTE98_RS09815 (RTE98_09815) cysD 2100065..2100981 (+) 917 Protein_1932 sulfate adenylyltransferase subunit CysD -
  RTE98_RS09820 (RTE98_09820) cysN 2100994..2102892 (+) 1899 WP_316959137.1 sulfate adenylyltransferase subunit CysN -
  RTE98_RS09825 (RTE98_09825) pilA 2103481..2103963 (-) 483 WP_042928458.1 pilin Machinery gene
  RTE98_RS09830 (RTE98_09830) pilB 2104274..2105977 (+) 1704 WP_267932281.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RTE98_RS09835 (RTE98_09835) pilC 2105979..2107196 (+) 1218 WP_267932282.1 type II secretion system F family protein Machinery gene
  RTE98_RS09840 (RTE98_09840) pilD 2107199..2108068 (+) 870 WP_316959139.1 A24 family peptidase Machinery gene
  RTE98_RS09845 (RTE98_09845) coaE 2108201..2108809 (+) 609 WP_316959140.1 dephospho-CoA kinase -
  RTE98_RS09850 (RTE98_09850) yacG 2108806..2109003 (+) 198 WP_102840588.1 DNA gyrase inhibitor YacG -
  RTE98_RS09855 (RTE98_09855) - 2109028..2109708 (-) 681 WP_316959141.1 energy-coupling factor ABC transporter permease -
  RTE98_RS09860 (RTE98_09860) - 2109747..2110784 (-) 1038 WP_316959142.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61888.82 Da        Isoelectric Point: 5.2370

>NTDB_id=891203 RTE98_RS09830 WP_267932281.1 2104274..2105977(+) (pilB) [Stutzerimonas frequens strain L27]
MSENTSLSGLARQMVAAGVLDEKTAQQAQTQAIRGKTPFVTYVVQNRLAKGRVIAELASEQFGVALLDLAALDKESLPKD
LVSEKLAKQHRVLPLSKRGTKLYVAVSDPANHQSIADIQFSTGLSIEPILVEDDKLGEAIDKLYDSGATGLEDLGEVDLD
GLDVETVSDDGRADDAGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYERAYRVRFRTDGILHEIARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEEQKALYMQALQQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEIDLPREALLEEGFP
ADKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=891203 RTE98_RS09830 WP_267932281.1 2104274..2105977(+) (pilB) [Stutzerimonas frequens strain L27]
ATGAGCGAGAATACTTCCCTTTCCGGGCTGGCGCGGCAGATGGTTGCGGCTGGTGTGCTGGACGAGAAGACGGCGCAGCA
GGCCCAGACTCAGGCCATTCGCGGCAAGACGCCCTTTGTCACCTACGTCGTCCAAAACCGGTTGGCCAAGGGCCGTGTCA
TTGCCGAACTAGCCTCCGAGCAGTTCGGTGTTGCGCTGCTGGATCTTGCTGCGCTGGATAAGGAGTCGCTGCCTAAAGAT
CTGGTGAGCGAGAAGCTGGCCAAGCAGCACCGGGTCCTTCCCCTGTCAAAGCGCGGCACCAAGCTGTATGTGGCGGTGTC
CGACCCTGCCAACCATCAATCGATTGCTGATATCCAGTTCAGCACCGGGCTTTCGATCGAGCCGATTCTGGTCGAGGACG
ACAAACTCGGGGAGGCGATCGACAAGCTCTACGATAGCGGTGCAACCGGCCTGGAGGACCTGGGAGAGGTCGATCTCGAT
GGGCTGGATGTCGAGACCGTTAGCGACGACGGTCGGGCGGATGATGCAGGGGAGGCCGCTGATGATGCCCCGGTCGTGCG
CTTCGTCAACAAGATGTTGCTCGATGCCATTCGTGGTGGTTCCTCCGACTTGCATTTTGAACCCTATGAAAGAGCCTATC
GCGTCCGGTTCCGCACCGATGGGATTCTGCACGAGATCGCTCGTCCCCCGATCCAGTTGGCGCCTCGCATATCGGCCCGT
CTGAAAGTGATGGCAGGGTTGGATATATCCGAGCGGCGTAAACCCCAGGACGGTCGGATCAAGATGAAGCTGTCCAAGTC
CAAGGCGATCGATTTTCGGGTCAATACCTTGCCTACGCTCTGGGGCGAGAAGATCGTGATGCGAATCCTCGATCCGTCCA
GTGCGCAGATGGGGATCGATGCGCTGGGGTACGAGGAAGAACAGAAGGCGCTCTATATGCAGGCGCTTCAGCAGCCACAG
GGCATGATACTGGTAACCGGCCCGACGGGTTCGGGCAAAACGGTATCCCTTTATACCGGTCTCAATATCCTCAATACGGT
CGACGTAAATATCTCCACCGCTGAGGATCCGGTGGAAATCAACCTGGAAGGTATCAACCAGGTCAACGTCAATCCCCGGC
AGGGGATGGACTTCTCCCAGGCGCTGCGCGCATTTCTACGGCAGGACCCCGATATCATCATGGTGGGTGAGATTCGGGAC
TTGGAAACCGCCGAGATCGCCATCAAGGCCGCGCAGACCGGGCACATGGTTATGTCGACGCTGCATACCAACAGCGCCGC
AGAAACCCTTACGCGCCTTCGCAACATGGGGGTGGCAGCGTTCAATATCGCTACGTCGGTGAACCTGATAATTGCTCAGC
GACTGGCGCGCAAGCTGTGTAACAGTTGCAAGAAAGAGATCGATCTGCCGCGCGAGGCTCTGTTGGAAGAGGGCTTCCCC
GCCGACAAGATTGGAACCTTCAAGATCTATGGTCCGGTTGGTTGCGACAATTGCAAAGGCGGTTACAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCAGCGTTGGCCAGGATTATCATGGAAGACGGTAATTCGATCGATATTTCCACCC
AGATGCGAAAAGACGGCTTCAATGACCTGCGCACCTCGGCTTTGCTCAAGGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.106

99.471

0.598
  pilB Acinetobacter baylyi ADP1

59.929

99.471

0.596
  pilB Legionella pneumophila strain ERS1305867

55.769

100

0.563
  pilB Vibrio cholerae strain A1552

50.178

99.118

0.497
  pilF Neisseria gonorrhoeae MS11

48.668

99.295

0.483
  pilB Vibrio campbellii strain DS40M4

47.902

100

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

51.823

91.887

0.476
  pilB/pilB1 Synechocystis sp. PCC 6803

39.272

92.063

0.362