Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OC457_RS01875 Genome accession   NZ_AP024854
Coordinates   375945..377630 (-) Length   561 a.a.
NCBI ID   WP_080174495.1    Uniprot ID   A0A1T4SLX5
Organism   Photobacterium toruni strain CECT 9189     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 370945..382630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OC457_RS01845 mutT 371558..371995 (-) 438 WP_235866930.1 8-oxo-dGTP diphosphatase MutT -
  OC457_RS01850 yacG 372115..372315 (-) 201 WP_080174490.1 DNA gyrase inhibitor YacG -
  OC457_RS01855 zapD 372323..373060 (-) 738 WP_080174491.1 cell division protein ZapD -
  OC457_RS01860 coaE 373107..373712 (-) 606 WP_080174492.1 dephospho-CoA kinase -
  OC457_RS01865 pilD 373716..374588 (-) 873 WP_080174493.1 A24 family peptidase Machinery gene
  OC457_RS01870 pilC 374648..375868 (-) 1221 WP_080174494.1 type II secretion system F family protein Machinery gene
  OC457_RS01875 pilB 375945..377630 (-) 1686 WP_080174495.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OC457_RS20850 - 377630..378064 (-) 435 WP_080174496.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OC457_RS01885 - 378559..379935 (-) 1377 WP_080174497.1 MATE family efflux transporter -
  OC457_RS01890 - 380059..380991 (-) 933 WP_080174498.1 carbamate kinase -
  OC457_RS01895 ptcA 381065..382111 (-) 1047 WP_080174499.1 putrescine carbamoyltransferase -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62491.01 Da        Isoelectric Point: 5.0303

>NTDB_id=88833 OC457_RS01875 WP_080174495.1 375945..377630(-) (pilB) [Photobacterium toruni strain CECT 9189]
MQTNLASILFQADLISREQEQQIVESVTAEGINVPTAITRLGIMADDELARSLSHLFNLDRVDVHHYDFQGCCQSVYQRE
LVLRHRVLPLAQDNSTLFVGLSDPTNVDAQDEFRFAIGKNVEPLLLDNKQLESAIRRVYGSDIGEVSNSRQISDEDLSNL
IDINDLELDESTDLSQDSAPVTRYINQVLMDALRRKASDIHFEPYETSYRIRFRCDGILHQHLTPPASLSRRLSTRLKVM
SKLNIAERRQPQDGRIKLKLSDTLAIDLRVSTLPTLWGEKVVLRILDSSSASLDIDILGYSETQKQAYLAALQRPQGMIL
MTGPTGSGKTVSLYTGLSILNTDERNISTAEDPVEINLQGINQVQINNQAGLGFAQALRSFLRQDPDVVMVGEIRDLETA
EIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGVEDFNLASSLSLIIAQRLARRLCSHCKQPHLLDDLTCERLKLPLDTT
VYQAHKEGCDDCNKGYIGRVGIYEVMPFSRDIAEALMQKATSLQLEDIAASQGMQRLRESGIDKLVAGITSLTELQRVLQ
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=88833 OC457_RS01875 WP_080174495.1 375945..377630(-) (pilB) [Photobacterium toruni strain CECT 9189]
ATGCAAACCAATCTCGCCTCGATCCTGTTCCAAGCTGACTTAATCAGCCGCGAGCAGGAGCAGCAAATCGTCGAGTCGGT
GACGGCGGAAGGGATCAATGTCCCGACCGCCATTACTCGCTTAGGCATCATGGCGGATGATGAACTGGCACGTTCATTAT
CGCACCTGTTCAACCTAGATCGAGTGGATGTTCACCATTATGACTTTCAAGGTTGCTGTCAAAGTGTCTACCAACGTGAA
CTGGTGTTACGCCATCGCGTACTGCCGCTTGCGCAAGACAATAGCACCCTTTTTGTTGGCCTTAGCGATCCTACCAATGT
CGACGCACAGGATGAGTTTCGCTTCGCTATTGGTAAGAACGTTGAACCATTATTATTAGATAATAAACAACTTGAATCCG
CGATTCGACGGGTTTACGGCAGTGATATTGGCGAGGTTAGTAATAGCCGTCAAATCAGTGATGAAGATCTGAGTAACTTA
ATTGATATTAACGATCTAGAGCTTGATGAATCCACTGATTTAAGCCAAGACAGCGCCCCCGTTACTCGTTATATCAACCA
AGTATTAATGGATGCGCTACGGCGTAAAGCGTCAGATATTCACTTTGAACCTTATGAAACCAGCTATCGAATTCGATTTC
GGTGTGATGGTATTTTACACCAGCATTTAACGCCACCAGCAAGTTTATCGCGGCGGTTATCGACCCGTTTAAAGGTCATG
TCAAAACTCAATATTGCCGAGCGTCGCCAACCGCAAGATGGACGGATAAAACTTAAATTATCTGACACATTGGCAATTGA
TCTACGCGTTTCTACTCTGCCAACCTTGTGGGGCGAAAAAGTCGTACTGCGAATTTTAGACAGCTCCAGTGCCAGCTTAG
ACATTGATATTTTAGGCTATAGCGAAACCCAAAAGCAGGCTTATTTAGCCGCTCTCCAGCGCCCGCAAGGCATGATCCTA
ATGACCGGTCCAACCGGTAGTGGTAAGACCGTATCGCTATACACAGGCTTAAGTATTTTAAACACTGATGAGCGCAATAT
TTCTACCGCTGAAGATCCAGTAGAGATCAACCTGCAAGGCATTAACCAAGTTCAAATTAATAATCAAGCGGGACTTGGAT
TTGCCCAAGCCTTGCGATCGTTTCTACGTCAAGATCCTGATGTGGTGATGGTCGGTGAGATTCGTGATTTAGAAACCGCT
GAAATCGCGGTAAAGGCAGCACAAACAGGACACTTAGTATTATCAACGCTGCACACCAACTCCGCCGCCGAAACGATTAC
CCGTTTAACGAATATGGGAGTGGAAGATTTTAATCTCGCATCATCACTGAGTTTGATTATAGCGCAACGATTAGCGCGCC
GATTATGCAGTCATTGTAAACAACCACACTTGCTTGATGATCTCACCTGTGAGCGTTTAAAACTGCCACTCGATACAACT
GTCTATCAAGCGCATAAAGAAGGCTGTGATGATTGTAATAAAGGCTACATTGGACGAGTGGGTATTTATGAAGTGATGCC
ATTTAGCCGTGATATTGCCGAAGCATTAATGCAAAAAGCCACCAGCTTACAGCTAGAAGATATTGCTGCCAGTCAAGGCA
TGCAACGGCTGCGAGAATCAGGGATAGATAAACTGGTTGCAGGCATTACTAGCCTCACTGAATTACAACGGGTACTGCAA
CTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)

Type II/IV secretion system protein (T2SSE)

(187-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T4SLX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.5

99.822

0.624
  pilB Vibrio parahaemolyticus RIMD 2210633

61.21

100

0.613
  pilB Vibrio campbellii strain DS40M4

59.821

99.822

0.597
  pilB Acinetobacter baumannii D1279779

53.95

92.513

0.499
  pilB Acinetobacter baylyi ADP1

51.451

92.157

0.474
  pilB Legionella pneumophila strain ERS1305867

46.986

100

0.472
  pilF Neisseria gonorrhoeae MS11

45.293

100

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

39.806

91.8

0.365
  pilF Thermus thermophilus HB27

36.17

100

0.364

Multiple sequence alignment