Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RS400_RS05350 Genome accession   NZ_CP135602
Coordinates   1136218..1137360 (+) Length   380 a.a.
NCBI ID   WP_005408369.1    Uniprot ID   A0A854DW57
Organism   Stenotrophomonas maltophilia strain SI1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1131218..1142360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RS400_RS05325 (RS400_05325) - 1131722..1132324 (-) 603 WP_049397188.1 short chain dehydrogenase -
  RS400_RS05330 (RS400_05330) - 1132423..1133298 (+) 876 WP_060381038.1 LysR family transcriptional regulator -
  RS400_RS05335 (RS400_05335) proC 1133475..1134296 (-) 822 WP_060381039.1 pyrroline-5-carboxylate reductase -
  RS400_RS05340 (RS400_05340) - 1134315..1134992 (-) 678 WP_049397192.1 YggS family pyridoxal phosphate-dependent enzyme -
  RS400_RS05345 (RS400_05345) pilT 1135080..1136117 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  RS400_RS05350 (RS400_05350) pilU 1136218..1137360 (+) 1143 WP_005408369.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RS400_RS05355 (RS400_05355) - 1137568..1138551 (+) 984 WP_114618884.1 aldo/keto reductase -
  RS400_RS05360 (RS400_05360) - 1138599..1139498 (-) 900 WP_133050767.1 LysR family transcriptional regulator -
  RS400_RS05365 (RS400_05365) - 1139592..1139801 (+) 210 WP_005412490.1 DUF4287 domain-containing protein -
  RS400_RS05370 (RS400_05370) - 1139912..1140619 (+) 708 WP_014036228.1 YitT family protein -
  RS400_RS05375 (RS400_05375) - 1140703..1141248 (-) 546 WP_315806046.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42355.69 Da        Isoelectric Point: 6.7683

>NTDB_id=887144 RS400_RS05350 WP_005408369.1 1136218..1137360(+) (pilU) [Stenotrophomonas maltophilia strain SI1]
MNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAI
GLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTG
HIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNA
NQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLG
MKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=887144 RS400_RS05350 WP_005408369.1 1136218..1137360(+) (pilU) [Stenotrophomonas maltophilia strain SI1]
GTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCGGACCTGTTCAT
CACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACGCAGACCCCGCTCACGCCGCAGCAGA
GCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAATGCAACTTCGCCATC
GGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGTCGCATCGA
GACGCGCATTCCGACGGTGGAAGAACTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCC
TGTTCGTCGGCGCCACCGGCACCGGTAAATCGACGTCGCTGGCAGCGATGATCGGTTACCGCAACCAGAACTCGACCGGC
CACATCATCACCATCGAAGATCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCAT
CGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGTGAGGTGCGTA
CCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCATGCCAACAACGCC
AACCAGGCGATGGACCGCATCGTCAATTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGACCTGTCGCTGAATCT
CAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTCGCGCAAGGTGGCGATGGAGATCCTGCTGGGCA
CGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATGAAGGACTCGGTCCAGCTGGGC
ATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGTTACGCCGA
TTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCACGCACGCTGTCACAGGGCCTGGATGGCG
TGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A854DW57

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.847

96.579

0.655
  pilU Acinetobacter baylyi ADP1

63.585

93.947

0.597
  pilU Vibrio cholerae strain A1552

53.371

93.684

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

93.421

0.403