Detailed information
Bioinformatically predicted
Overview
| Name | comEA/celA/cilE | Type | Machinery gene |
| Locus tag | RSK81_RS03545 | Genome accession | NZ_CP135591 |
| Coordinates | 696665..697345 (+) | Length | 226 a.a. |
| NCBI ID | WP_315687453.1 | Uniprot ID | A0AA96NSG3 |
| Organism | Streptococcus sp. DTU_2020_1000888_1_SI_GRL_NUU_041A | ||
| Function | dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| ICE | 695682..753271 | 696665..697345 | within | 0 |
Gene organization within MGE regions
Location: 695682..753271
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| RSK81_RS03540 (RSK81_03540) | - | 695968..696516 (+) | 549 | WP_315687451.1 | HXXEE domain-containing protein | - |
| RSK81_RS03545 (RSK81_03545) | comEA/celA/cilE | 696665..697345 (+) | 681 | WP_315687453.1 | helix-hairpin-helix domain-containing protein | Machinery gene |
| RSK81_RS03550 (RSK81_03550) | comEC/celB | 697329..699569 (+) | 2241 | WP_315687455.1 | DNA internalization-related competence protein ComEC/Rec2 | Machinery gene |
| RSK81_RS03555 (RSK81_03555) | - | 699763..699912 (+) | 150 | WP_314580277.1 | hypothetical protein | - |
| RSK81_RS03560 (RSK81_03560) | - | 699950..700450 (+) | 501 | WP_315687457.1 | modification methylase Sau96I | - |
| RSK81_RS03565 (RSK81_03565) | - | 700462..700611 (+) | 150 | WP_268715203.1 | hypothetical protein | - |
| RSK81_RS03570 (RSK81_03570) | - | 700615..700929 (+) | 315 | WP_314580282.1 | hypothetical protein | - |
| RSK81_RS03575 (RSK81_03575) | - | 700931..701602 (+) | 672 | WP_315687460.1 | DUF2786 domain-containing protein | - |
| RSK81_RS03580 (RSK81_03580) | - | 701636..703126 (+) | 1491 | WP_105025994.1 | primase C-terminal domain-containing protein | - |
| RSK81_RS03585 (RSK81_03585) | - | 703244..703444 (+) | 201 | WP_008809739.1 | hypothetical protein | - |
| RSK81_RS03590 (RSK81_03590) | - | 703458..703646 (+) | 189 | WP_315686410.1 | hypothetical protein | - |
| RSK81_RS03595 (RSK81_03595) | - | 703630..704046 (+) | 417 | WP_315686409.1 | hypothetical protein | - |
| RSK81_RS03600 (RSK81_03600) | - | 704043..704483 (+) | 441 | WP_060971013.1 | thioredoxin domain-containing protein | - |
| RSK81_RS03605 (RSK81_03605) | - | 704480..704707 (+) | 228 | WP_023916842.1 | hypothetical protein | - |
| RSK81_RS03610 (RSK81_03610) | - | 704718..704918 (+) | 201 | WP_315687463.1 | hypothetical protein | - |
| RSK81_RS03615 (RSK81_03615) | - | 704935..705948 (+) | 1014 | WP_315687464.1 | hypothetical protein | - |
| RSK81_RS03620 (RSK81_03620) | - | 706076..706351 (+) | 276 | WP_168955504.1 | hypothetical protein | - |
| RSK81_RS03625 (RSK81_03625) | - | 706761..707099 (+) | 339 | WP_315687466.1 | hypothetical protein | - |
| RSK81_RS03630 (RSK81_03630) | - | 707092..707436 (+) | 345 | WP_168955502.1 | hypothetical protein | - |
| RSK81_RS03635 (RSK81_03635) | - | 707436..708230 (+) | 795 | WP_315687468.1 | DNA/RNA non-specific endonuclease | - |
| RSK81_RS03640 (RSK81_03640) | - | 708252..708725 (+) | 474 | WP_315686400.1 | hypothetical protein | - |
| RSK81_RS03645 (RSK81_03645) | mobP2 | 708742..711108 (+) | 2367 | WP_410530916.1 | MobP2 family relaxase | - |
| RSK81_RS03650 (RSK81_03650) | - | 711218..711478 (+) | 261 | WP_136115662.1 | hypothetical protein | - |
| RSK81_RS03655 (RSK81_03655) | - | 711497..716125 (+) | 4629 | WP_315687472.1 | PBECR4 domain-containing protein | - |
| RSK81_RS03660 (RSK81_03660) | - | 716319..718076 (+) | 1758 | WP_315687473.1 | type IA DNA topoisomerase | - |
| RSK81_RS03665 (RSK81_03665) | - | 718070..719893 (+) | 1824 | WP_315686392.1 | ATP-dependent Clp protease ATP-binding subunit | - |
| RSK81_RS03670 (RSK81_03670) | - | 719990..720337 (+) | 348 | WP_315686390.1 | TrbC/VirB2 family protein | - |
| RSK81_RS03675 (RSK81_03675) | - | 720355..721437 (+) | 1083 | WP_315686389.1 | hypothetical protein | - |
| RSK81_RS03680 (RSK81_03680) | - | 721458..722279 (+) | 822 | WP_315687474.1 | LPXTG cell wall anchor domain-containing protein | - |
| RSK81_RS03685 (RSK81_03685) | - | 722295..722759 (+) | 465 | WP_105025989.1 | single-stranded DNA-binding protein | - |
| RSK81_RS03690 (RSK81_03690) | - | 722983..723234 (+) | 252 | WP_315687476.1 | hypothetical protein | - |
| RSK81_RS03695 (RSK81_03695) | - | 723227..726367 (+) | 3141 | WP_315687478.1 | VirD4-like conjugal transfer protein, CD1115 family | - |
| RSK81_RS03700 (RSK81_03700) | - | 726465..726947 (+) | 483 | WP_168955492.1 | lipoprotein | - |
| RSK81_RS03705 (RSK81_03705) | - | 727067..729106 (+) | 2040 | WP_410530912.1 | pLS20_p028 family conjugation system transmembrane protein | - |
| RSK81_RS03710 (RSK81_03710) | - | 729113..729397 (+) | 285 | WP_168955491.1 | BRCT domain-containing protein | - |
| RSK81_RS03715 (RSK81_03715) | - | 729588..729914 (+) | 327 | WP_168955490.1 | DUF5592 family protein | - |
| RSK81_RS03720 (RSK81_03720) | - | 729905..730555 (+) | 651 | WP_168955489.1 | hypothetical protein | - |
| RSK81_RS03725 (RSK81_03725) | - | 730565..732514 (+) | 1950 | WP_168955488.1 | virulence factor | - |
| RSK81_RS03730 (RSK81_03730) | - | 732532..732789 (+) | 258 | WP_168955487.1 | hypothetical protein | - |
| RSK81_RS03735 (RSK81_03735) | - | 732865..734166 (+) | 1302 | WP_410530917.1 | CHAP domain-containing protein | - |
| RSK81_RS03740 (RSK81_03740) | - | 734179..734769 (+) | 591 | WP_315687482.1 | hypothetical protein | - |
| RSK81_RS03745 (RSK81_03745) | - | 734937..735755 (+) | 819 | WP_315687483.1 | ParA family protein | - |
| RSK81_RS03750 (RSK81_03750) | - | 735752..736024 (+) | 273 | WP_168955483.1 | hypothetical protein | - |
| RSK81_RS03755 (RSK81_03755) | - | 736037..736345 (+) | 309 | WP_315686376.1 | hypothetical protein | - |
| RSK81_RS03760 (RSK81_03760) | - | 736460..737788 (+) | 1329 | WP_315686374.1 | ISLre2 family transposase | - |
| RSK81_RS03765 (RSK81_03765) | - | 738208..739305 (+) | 1098 | WP_049547456.1 | DUF805 domain-containing protein | - |
| RSK81_RS03770 (RSK81_03770) | holA | 739556..740602 (+) | 1047 | WP_049547457.1 | DNA polymerase III subunit delta | - |
| RSK81_RS03775 (RSK81_03775) | sodA | 740665..741270 (+) | 606 | WP_002906995.1 | superoxide dismutase SodA | - |
| RSK81_RS03780 (RSK81_03780) | - | 741406..742485 (+) | 1080 | WP_315687484.1 | serine hydrolase | - |
| RSK81_RS03785 (RSK81_03785) | - | 742696..742836 (+) | 141 | WP_002897970.1 | hypothetical protein | - |
| RSK81_RS03790 (RSK81_03790) | - | 743102..744682 (+) | 1581 | WP_315687486.1 | ABC transporter ATP-binding protein | - |
| RSK81_RS03795 (RSK81_03795) | - | 744697..745182 (+) | 486 | WP_315687487.1 | hypothetical protein | - |
| RSK81_RS03800 (RSK81_03800) | - | 745355..747154 (+) | 1800 | WP_002915643.1 | serine hydrolase domain-containing protein | - |
| RSK81_RS03805 (RSK81_03805) | - | 747185..748009 (+) | 825 | WP_315687489.1 | CPBP family intramembrane glutamic endopeptidase | - |
| RSK81_RS03810 (RSK81_03810) | - | 748138..749046 (+) | 909 | WP_002897963.1 | CPBP family intramembrane glutamic endopeptidase | - |
| RSK81_RS03815 (RSK81_03815) | - | 749249..749536 (+) | 288 | WP_002897962.1 | autorepressor SdpR family transcription factor | - |
| RSK81_RS03820 (RSK81_03820) | - | 749514..750023 (+) | 510 | WP_002915640.1 | hypothetical protein | - |
| RSK81_RS03825 (RSK81_03825) | - | 750518..751306 (+) | 789 | WP_125340720.1 | YhfC family intramembrane metalloprotease | - |
| RSK81_RS03830 (RSK81_03830) | - | 751284..751565 (+) | 282 | WP_315687491.1 | autorepressor SdpR family transcription factor | - |
| RSK81_RS03835 (RSK81_03835) | - | 751552..752049 (+) | 498 | WP_315687493.1 | DUF1648 domain-containing protein | - |
| RSK81_RS03840 (RSK81_03840) | - | 752097..752783 (+) | 687 | WP_049544039.1 | Crp/Fnr family transcriptional regulator | - |
Sequence
Protein
Download Length: 226 a.a. Molecular weight: 23911.59 Da Isoelectric Point: 4.6491
>NTDB_id=887026 RSK81_RS03545 WP_315687453.1 696665..697345(+) (comEA/celA/cilE) [Streptococcus sp. DTU_2020_1000888_1_SI_GRL_NUU_041A]
MLEEIIEKLKEYKLFVGLAVVGAVLGGFFLIKGNHQPQNQVAALSQEITSSSSSADEESEKVLSKAKADEEESDQVTVDI
KGAVKNPGVYELRAGARVHEAIQKAGGLTADAEAKSINQAQKLTDEAVIYVAKIGEEGADVTQTGQHQAGASDSAGSSGG
QSDKVNLNTATEAELQTISGIGQKRASDIIAYRESNGRFKSVDDLKKVSGIGDKTLEKLKEYVTVD
MLEEIIEKLKEYKLFVGLAVVGAVLGGFFLIKGNHQPQNQVAALSQEITSSSSSADEESEKVLSKAKADEEESDQVTVDI
KGAVKNPGVYELRAGARVHEAIQKAGGLTADAEAKSINQAQKLTDEAVIYVAKIGEEGADVTQTGQHQAGASDSAGSSGG
QSDKVNLNTATEAELQTISGIGQKRASDIIAYRESNGRFKSVDDLKKVSGIGDKTLEKLKEYVTVD
Nucleotide
Download Length: 681 bp
>NTDB_id=887026 RSK81_RS03545 WP_315687453.1 696665..697345(+) (comEA/celA/cilE) [Streptococcus sp. DTU_2020_1000888_1_SI_GRL_NUU_041A]
ATGCTTGAAGAAATCATTGAAAAGCTAAAAGAATACAAGCTCTTTGTCGGTCTGGCAGTAGTTGGCGCTGTGCTAGGTGG
TTTTTTCCTCATCAAAGGAAATCATCAACCGCAAAATCAGGTGGCCGCCTTGAGTCAAGAAATAACTTCCTCATCCAGCT
CAGCTGATGAAGAGAGTGAAAAAGTTCTTTCTAAGGCTAAGGCGGACGAAGAGGAAAGCGATCAGGTCACTGTGGATATT
AAAGGCGCAGTTAAGAATCCTGGTGTCTATGAGCTGAGAGCGGGAGCGAGAGTCCATGAAGCCATCCAAAAAGCTGGCGG
ATTGACAGCAGATGCCGAAGCTAAGTCCATCAATCAGGCGCAAAAATTAACGGATGAAGCAGTAATCTATGTGGCAAAGA
TTGGTGAAGAAGGAGCTGATGTCACACAGACTGGCCAGCATCAAGCAGGAGCTTCTGATTCTGCTGGTTCCAGTGGTGGA
CAGTCGGACAAGGTCAATCTAAATACGGCGACAGAGGCTGAATTACAGACCATTTCAGGCATCGGACAGAAGCGAGCTTC
TGACATCATAGCCTACCGCGAAAGCAACGGCCGCTTCAAGTCAGTAGATGATCTGAAAAAGGTGTCAGGGATTGGAGATA
AGACCTTAGAAAAGCTGAAAGAATATGTCACAGTGGATTAA
ATGCTTGAAGAAATCATTGAAAAGCTAAAAGAATACAAGCTCTTTGTCGGTCTGGCAGTAGTTGGCGCTGTGCTAGGTGG
TTTTTTCCTCATCAAAGGAAATCATCAACCGCAAAATCAGGTGGCCGCCTTGAGTCAAGAAATAACTTCCTCATCCAGCT
CAGCTGATGAAGAGAGTGAAAAAGTTCTTTCTAAGGCTAAGGCGGACGAAGAGGAAAGCGATCAGGTCACTGTGGATATT
AAAGGCGCAGTTAAGAATCCTGGTGTCTATGAGCTGAGAGCGGGAGCGAGAGTCCATGAAGCCATCCAAAAAGCTGGCGG
ATTGACAGCAGATGCCGAAGCTAAGTCCATCAATCAGGCGCAAAAATTAACGGATGAAGCAGTAATCTATGTGGCAAAGA
TTGGTGAAGAAGGAGCTGATGTCACACAGACTGGCCAGCATCAAGCAGGAGCTTCTGATTCTGCTGGTTCCAGTGGTGGA
CAGTCGGACAAGGTCAATCTAAATACGGCGACAGAGGCTGAATTACAGACCATTTCAGGCATCGGACAGAAGCGAGCTTC
TGACATCATAGCCTACCGCGAAAGCAACGGCCGCTTCAAGTCAGTAGATGATCTGAAAAAGGTGTCAGGGATTGGAGATA
AGACCTTAGAAAAGCTGAAAGAATATGTCACAGTGGATTAA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| comEA/celA/cilE | Streptococcus pneumoniae Rx1 |
50.862 |
100 |
0.522 |
| comEA/celA/cilE | Streptococcus pneumoniae D39 |
50.862 |
100 |
0.522 |
| comEA/celA/cilE | Streptococcus pneumoniae R6 |
50.862 |
100 |
0.522 |
| comEA/celA/cilE | Streptococcus pneumoniae TIGR4 |
50.431 |
100 |
0.518 |
| comEA | Streptococcus thermophilus LMD-9 |
49.569 |
100 |
0.509 |
| comEA/celA/cilE | Streptococcus mitis SK321 |
48.707 |
100 |
0.5 |
| comEA/celA/cilE | Streptococcus mitis NCTC 12261 |
49.778 |
99.558 |
0.496 |
| comEA | Lactococcus lactis subsp. cremoris KW2 |
41.778 |
99.558 |
0.416 |
| comEA | Bacillus subtilis subsp. subtilis str. 168 |
42.534 |
97.788 |
0.416 |
| comEA | Latilactobacillus sakei subsp. sakei 23K |
34.855 |
100 |
0.372 |