Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QYQ95_RS02585 Genome accession   NZ_CP135176
Coordinates   512002..513687 (-) Length   561 a.a.
NCBI ID   WP_016800654.1    Uniprot ID   A0AAN0NAA0
Organism   Vibrio cyclitrophicus ZF270     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 507002..518687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYQ95_RS02555 (QYQ95_02555) rplS 507545..507898 (+) 354 WP_004735514.1 50S ribosomal protein L19 -
  QYQ95_RS02560 (QYQ95_02560) yacG 508159..508356 (-) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  QYQ95_RS02565 (QYQ95_02565) zapD 508438..509178 (-) 741 WP_010435917.1 cell division protein ZapD -
  QYQ95_RS02570 (QYQ95_02570) coaE 509205..509819 (-) 615 WP_016800649.1 dephospho-CoA kinase -
  QYQ95_RS02575 (QYQ95_02575) pilD 509822..510691 (-) 870 WP_016768203.1 A24 family peptidase Machinery gene
  QYQ95_RS02580 (QYQ95_02580) pilC 510758..511987 (-) 1230 WP_016800652.1 type II secretion system F family protein Machinery gene
  QYQ95_RS02585 (QYQ95_02585) pilB 512002..513687 (-) 1686 WP_016800654.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QYQ95_RS02590 (QYQ95_02590) pilA 513687..514160 (-) 474 WP_016800656.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  QYQ95_RS02595 (QYQ95_02595) nadC 514414..515304 (-) 891 WP_016800658.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QYQ95_RS02600 (QYQ95_02600) ampD 515388..515987 (+) 600 WP_016791286.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QYQ95_RS02605 (QYQ95_02605) pdhR 516469..517230 (+) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62103.88 Da        Isoelectric Point: 5.3122

>NTDB_id=885972 QYQ95_RS02585 WP_016800654.1 512002..513687(-) (pilB) [Vibrio cyclitrophicus ZF270]
MLTNLPTVLRQAGLLSLTQEQAVAEHVEASGISTPEALLALNFFSSDSLANNIKTIFGLPLVRLANTDYESLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQVELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVKVSDDEITPIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHFRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKETDDSPDIFLRHSIPNSQI
IYKANSQGCNECNQGYSGRVGIYEVMPFTDKLKTSLINKSNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=885972 QYQ95_RS02585 WP_016800654.1 512002..513687(-) (pilB) [Vibrio cyclitrophicus ZF270]
ATGTTAACCAACCTCCCAACAGTTCTCCGTCAGGCTGGCTTACTTAGCCTGACTCAGGAACAAGCTGTTGCGGAACATGT
GGAAGCTTCAGGTATATCTACACCTGAAGCTTTACTCGCTCTGAATTTCTTCAGCAGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCGTTAGTACGATTAGCCAATACTGACTACGAATCCCTGTGTGAACAATTAGGACTTCGTGAA
CTGATTACTAAGTATCGTGCTATTCCAATTTCAGTTTCTAGCTCAACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAGGCTGAAGATGACTTCCGCTTTGCGACAGGATTGCAAGTTGAATTGGTTGTCGCTAACTACTCTGAATTAGAAGGTG
CGATACGCAAGCTGTATGGCCGCTCTATTTCCGGGCAAGATTCTAAGCGTAAGGAGATCACCCAAGATGAGTTAGCTAAC
CTCGTGAAGGTATCTGACGACGAAATAACGCCGATTGAAGATCTCAGCCAAGATGACTCTCCTGTGAGTCGCTTTATCAA
TCAGATCTTGGTTGATGCGGTACGTAAAGGCGCATCGGACATCCACTTCGAACCTTATGAAGAACACTTCCGTGTACGCC
TGCGTTGTGATGGCATATTGGTTGAAATCCAACAGCCCGCCTCTCATTTAAGCCGTCGTCTGTCAGCTCGCTTAAAGATC
CTCGCTAAACTCGATATTGCCGAACGTCGCTTACCTCAAGATGGCCGTATCAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGCGTATCAACGCTGCCTACCTTATGGGGCGAGAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATCGACAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGACTTCGAGTACTCAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCAGTAGAGATTAACCTGTGTGGTATCAATCAGGTACAGGTGACGCCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGCTCATTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCCGAAATCGCGATCAAAGCCTCCCAAACCGGGCATTTAGTACTTTCAACACTGCACACTAACTCAGCCGCAGAAACCGT
GACTCGGCTTGCTCATATGGGAATAGAGCCCTTCAACCTTGCTTCGTCATTGAGCCTAATCATAGCCCAACGACTAGCAA
GACGCTTGTGCAACCATTGCAAAGAAACAGACGACTCGCCGGACATATTTCTGCGTCACTCGATTCCTAACAGCCAAATA
ATCTACAAAGCTAACTCTCAAGGGTGTAATGAATGTAACCAAGGTTACTCAGGCCGAGTGGGTATCTATGAAGTGATGCC
GTTTACCGACAAGCTAAAAACCAGCCTTATCAACAAATCCAATGCGCTAGCGATTGAAGATCTCGCGCGTAGAGAAGGCA
TGCGCACTCTGCAAGAGTCAGGGCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.759

100

0.742
  pilB Vibrio campbellii strain DS40M4

70.996

100

0.711
  pilB Vibrio parahaemolyticus RIMD 2210633

70.819

100

0.709
  pilB Legionella pneumophila strain ERS1305867

47.826

98.396

0.471
  pilB Acinetobacter baumannii D1279779

50.775

91.979

0.467
  pilB Acinetobacter baylyi ADP1

49.43

93.761

0.463
  pilF Neisseria gonorrhoeae MS11

44.464

99.822

0.444
  pilF Thermus thermophilus HB27

39.272

93.048

0.365