Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   RHM62_RS04435 Genome accession   NZ_CP133777
Coordinates   932413..933645 (-) Length   410 a.a.
NCBI ID   WP_322124355.1    Uniprot ID   -
Organism   Actimicrobium sp. CCC2.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 927413..938645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM62_RS04405 (RHM62_04405) - 928570..929436 (+) 867 WP_322124350.1 ATP-binding protein -
  RHM62_RS04410 (RHM62_04410) - 929429..929854 (+) 426 WP_322124351.1 NUDIX domain-containing protein -
  RHM62_RS04415 (RHM62_04415) yacG 929860..930054 (-) 195 WP_322124352.1 DNA gyrase inhibitor YacG -
  RHM62_RS04420 (RHM62_04420) zapD 930080..930835 (-) 756 WP_322124353.1 cell division protein ZapD -
  RHM62_RS04425 (RHM62_04425) coaE 930927..931550 (-) 624 WP_322124354.1 dephospho-CoA kinase -
  RHM62_RS04430 (RHM62_04430) pilD 931547..932413 (-) 867 WP_322125317.1 A24 family peptidase Machinery gene
  RHM62_RS04435 (RHM62_04435) pilG 932413..933645 (-) 1233 WP_322124355.1 type II secretion system F family protein Machinery gene
  RHM62_RS04440 (RHM62_04440) pilB 933669..935393 (-) 1725 WP_322124356.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHM62_RS04445 (RHM62_04445) - 935569..936870 (-) 1302 WP_322124357.1 HlyC/CorC family transporter -
  RHM62_RS04450 (RHM62_04450) ispB 936988..937977 (-) 990 WP_322124358.1 octaprenyl diphosphate synthase -
  RHM62_RS04455 (RHM62_04455) rplU 938216..938527 (+) 312 WP_009665832.1 50S ribosomal protein L21 -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44676.87 Da        Isoelectric Point: 10.1609

>NTDB_id=876645 RHM62_RS04435 WP_322124355.1 932413..933645(-) (pilG) [Actimicrobium sp. CCC2.4]
MATAARKTVGSLAIKESIYAWEGKDKSGKIVRGEMRANGEAIVNATLRRQGVMTTKVKKKTFRGGKKITDKDISLFTRQL
ATMMKAGVPLLQSFDIVAKGHANPSMSKLIMEIRSDVETGTSLNQAFRKFPLYFDPLFCNLVGAGEQAGILEDLLTRLAI
YKEKTIAIKGKIKSALFYPISILVVAFVVTAVIMIFVVPAFKEVFKSFGADLPGPTLVVMAISDFFVAYWYLIFGLLFGS
FYMFFQAWRRSLKVQRFMDKALLQAPIFGAVIRKATIARWTRTLATMFAAGVPLVESLDSVGGAAGNAVYLDATKKIQTE
VSTGTSLTVAMQNANVFPNMVTQMVSIGEESGSLDNMLGKVADFYENEVDDAVAAISSLMEPLIMVVLGVIIGGLVIAMY
LPIFKLGAVV

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=876645 RHM62_RS04435 WP_322124355.1 932413..933645(-) (pilG) [Actimicrobium sp. CCC2.4]
ATGGCAACTGCAGCCCGAAAAACGGTGGGTTCACTGGCAATCAAGGAATCGATCTATGCCTGGGAAGGCAAAGACAAGTC
CGGCAAGATTGTCCGTGGTGAAATGCGCGCCAATGGCGAAGCGATCGTCAATGCCACGCTGCGTCGCCAGGGTGTGATGA
CGACCAAGGTCAAGAAAAAAACCTTCCGCGGCGGCAAAAAAATCACCGACAAGGACATCAGCCTGTTCACCCGGCAGCTG
GCCACGATGATGAAGGCCGGCGTGCCGCTGCTGCAATCCTTCGATATTGTCGCCAAGGGCCACGCCAATCCGTCGATGTC
GAAACTGATCATGGAAATCCGTAGCGATGTCGAAACCGGTACCAGCCTGAACCAGGCCTTCCGCAAATTTCCGCTGTACT
TCGACCCGCTATTCTGCAACCTGGTCGGTGCCGGCGAACAAGCCGGTATTCTTGAAGACCTGTTGACCCGACTGGCGATC
TACAAAGAAAAAACTATCGCCATCAAAGGCAAGATCAAGTCGGCCCTGTTCTATCCGATCTCGATCCTGGTCGTGGCCTT
CGTCGTCACCGCCGTCATCATGATTTTCGTGGTACCGGCCTTCAAGGAAGTGTTCAAGAGTTTCGGTGCCGATTTGCCGG
GCCCGACGCTGGTCGTGATGGCGATCTCGGACTTCTTCGTGGCCTACTGGTACCTGATCTTCGGCCTGCTCTTCGGCAGT
TTTTATATGTTCTTCCAGGCCTGGCGGCGCTCGCTCAAAGTCCAGCGTTTCATGGACAAGGCGCTGCTGCAGGCACCGAT
CTTCGGTGCCGTGATCCGCAAGGCCACGATCGCGCGCTGGACCCGTACGCTAGCGACGATGTTCGCCGCCGGCGTGCCGC
TGGTCGAATCGCTGGACTCGGTCGGCGGCGCTGCCGGTAATGCGGTGTATCTCGATGCCACCAAAAAAATCCAGACCGAA
GTCAGCACCGGTACCAGCCTGACGGTCGCCATGCAAAACGCCAACGTCTTCCCGAACATGGTCACGCAAATGGTCTCGAT
CGGCGAAGAATCCGGCTCGCTCGACAACATGCTCGGCAAGGTCGCCGACTTCTATGAAAACGAAGTCGACGATGCCGTTG
CCGCCATCTCCAGCCTGATGGAACCGCTCATCATGGTGGTCCTTGGTGTCATCATCGGCGGCCTGGTCATTGCGATGTAC
TTGCCGATCTTTAAATTGGGAGCCGTGGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)

Type II secretion system (T2SS), protein F (T2SSF)

(280-402)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

53.5

97.561

0.522
  pilC Legionella pneumophila strain ERS1305867

53.788

96.585

0.52
  pilC Pseudomonas stutzeri DSM 10701

52.868

97.805

0.517
  pilG Neisseria meningitidis 44/76-A

53

97.561

0.517
  pilC Acinetobacter baumannii D1279779

51.385

96.829

0.498
  pilC Acinetobacter baylyi ADP1

49.749

97.073

0.483
  pilC Vibrio cholerae strain A1552

39.364

99.756

0.393
  pilC Vibrio campbellii strain DS40M4

39.798

96.829

0.385