Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   TOL5_RS19730 Genome accession   NZ_AP024708
Coordinates   4253208..4255379 (-) Length   723 a.a.
NCBI ID   WP_121774349.1    Uniprot ID   -
Organism   Acinetobacter sp. Tol 5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4248208..4260379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOL5_RS19720 (TOL5_38940) - 4250625..4251494 (-) 870 WP_121774350.1 hypothetical protein -
  TOL5_RS19725 (TOL5_38950) aroB 4251513..4252592 (-) 1080 WP_004825381.1 3-dehydroquinate synthase -
  TOL5_RS22555 (TOL5_38960) aroK 4252629..4253171 (-) 543 WP_004825383.1 shikimate kinase AroK -
  TOL5_RS19730 (TOL5_38970) pilQ 4253208..4255379 (-) 2172 WP_121774349.1 type IV pilus secretin PilQ Machinery gene
  TOL5_RS19735 (TOL5_38980) comL 4255400..4255930 (-) 531 WP_005034344.1 pilus assembly protein PilP Machinery gene
  TOL5_RS19740 (TOL5_38990) comO 4255927..4256679 (-) 753 WP_121774348.1 type 4a pilus biogenesis protein PilO Machinery gene
  TOL5_RS19745 (TOL5_39000) pilN 4256676..4257317 (-) 642 WP_004825390.1 PilN domain-containing protein Machinery gene
  TOL5_RS19750 (TOL5_39010) comM 4257317..4258375 (-) 1059 WP_005034346.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 723 a.a.        Molecular weight: 78652.28 Da        Isoelectric Point: 7.2150

>NTDB_id=87604 TOL5_RS19730 WP_121774349.1 4253208..4255379(-) (pilQ) [Acinetobacter sp. Tol 5]
MKKSLKNILIGDGKFMNSQLRQFTMATVAIAITQVAAAQTTMTNIVSMKIPGQGTEIRVMFNGLPPQPQAYQLDNPSRLV
LDFDNVKQNISQASIPVASEEASTVDVAADDQRSRLTVNLKNSGAFTTRVEGNTFILKINNANQAPSLPSYVSPTVNKQN
QGIADIGFQRGNTGEGQVIVSLSGANTPVDVQQQGSKVVIRTLGNKIPTHLLRRLNVNDFATPVSSVDAYNEGSNGVITI
QSSGSYEYMAFQAENKLTVSLKRPAENKVARPSSSQSYTGKKISLDFQDIEVRRVLQLLADFTGINMVAADSVQGNITLR
LKDVPWDQALDIILKTKNLDKRRNGSVIWIAPVTELIKAEEDEAKALAQSVKLAPIQTEYIQLNYTKVADVLKLLEDSRN
PKGAEANRTTNTESLALESLLSLRGSAVADTRTNTLIINDTANNIDKIRKMIDLLDVSIKQVMVEARIVRATTDFTKEMG
VKWGILSQGITKNNDLLVGGSDQTLWDLRTPDDDGKYTITRPDNLNVDLGVTTTGASKIAFGLISMSDFMLDLELSALQS
DGYGEVISTPKVMTADKQKAKVSSGQQIPYQTTQTAGGAATATTSFKDALLSLDVTPSITPDGKVQMQLNITSDSPAGTA
PNGEIILNKNEVNTNVLVDNGETVVLGGIFEQETKSSQTKVPFLGDIPYLGRLFRKDLKSDNKRELLIFVTPRIVNDSRT
RNH

Nucleotide


Download         Length: 2172 bp        

>NTDB_id=87604 TOL5_RS19730 WP_121774349.1 4253208..4255379(-) (pilQ) [Acinetobacter sp. Tol 5]
ATGAAAAAGAGTTTGAAAAATATCTTGATTGGGGATGGTAAGTTTATGAATAGTCAACTTCGTCAATTTACTATGGCGAC
GGTCGCAATTGCAATTACACAAGTTGCAGCAGCACAAACCACGATGACCAACATTGTTTCGATGAAAATACCGGGGCAAG
GTACAGAAATTCGTGTCATGTTTAATGGTCTTCCTCCGCAGCCACAAGCCTATCAATTGGATAATCCATCTCGTTTAGTA
TTGGACTTTGACAATGTTAAACAAAATATTAGCCAAGCATCTATTCCCGTAGCGAGTGAGGAAGCTTCAACAGTTGATGT
CGCTGCAGATGATCAACGTTCTCGGTTAACAGTAAATTTAAAAAATAGTGGCGCTTTTACAACACGTGTAGAAGGCAATA
CTTTTATTTTAAAAATTAATAATGCCAATCAAGCACCGAGCTTACCAAGTTATGTATCTCCGACGGTCAATAAGCAAAAT
CAAGGGATTGCTGATATTGGCTTCCAACGTGGAAATACTGGCGAAGGACAAGTAATTGTGAGTCTTTCGGGTGCAAATAC
CCCGGTGGATGTTCAGCAGCAAGGTAGTAAGGTCGTTATTCGAACATTGGGAAATAAAATTCCTACACATTTATTGCGTC
GTTTAAATGTCAATGATTTTGCAACACCTGTCTCTAGCGTAGACGCTTATAACGAGGGTTCCAATGGTGTCATCACCATT
CAATCATCGGGTAGCTATGAATATATGGCTTTCCAAGCTGAAAATAAATTAACAGTCAGCTTGAAACGACCTGCAGAAAA
TAAGGTGGCTCGCCCAAGTTCAAGCCAATCTTATACAGGCAAGAAAATATCGCTAGATTTCCAAGATATTGAAGTCCGTC
GAGTGTTGCAGTTATTGGCAGACTTTACTGGCATCAATATGGTGGCTGCTGATTCGGTACAAGGCAATATCACCCTGCGT
TTGAAAGATGTGCCTTGGGATCAAGCTTTAGACATTATCCTAAAAACAAAGAATTTGGATAAACGCCGTAATGGGAGTGT
GATTTGGATTGCGCCTGTAACTGAGTTAATTAAAGCGGAAGAGGATGAAGCAAAGGCATTAGCACAAAGTGTAAAATTAG
CACCTATTCAAACTGAATATATTCAGCTGAATTACACCAAAGTGGCAGATGTTTTGAAATTACTGGAAGACTCTCGAAAT
CCGAAAGGGGCAGAAGCAAATCGAACCACCAATACAGAATCTTTAGCATTGGAAAGTCTTTTAAGTTTAAGAGGCAGTGC
AGTAGCTGATACACGAACAAATACTTTAATCATCAATGATACTGCGAATAATATTGATAAAATTCGTAAGATGATTGACT
TGCTTGATGTTTCTATTAAGCAAGTCATGGTTGAAGCGCGAATTGTACGTGCTACAACAGATTTTACCAAAGAAATGGGC
GTGAAATGGGGTATTTTATCTCAAGGCATCACCAAAAATAATGACCTTTTGGTGGGAGGGAGTGATCAAACCCTATGGGA
TTTAAGAACGCCAGATGATGATGGTAAATATACCATTACACGACCTGATAACTTGAATGTAGACCTTGGTGTTACAACAA
CGGGTGCGAGTAAAATAGCTTTTGGTTTAATCAGTATGTCTGATTTTATGTTGGACTTGGAGTTATCTGCTTTGCAATCT
GATGGGTATGGTGAAGTCATTTCAACACCTAAAGTGATGACTGCTGATAAACAAAAAGCCAAAGTTTCATCAGGTCAACA
AATTCCTTATCAAACAACACAAACTGCTGGTGGTGCAGCAACAGCAACCACATCATTTAAAGATGCATTATTAAGTCTGG
ATGTAACACCTAGCATTACCCCTGATGGTAAAGTGCAAATGCAGCTTAATATTACCAGCGACTCACCAGCAGGCACAGCA
CCTAATGGAGAAATTATTCTGAATAAGAATGAGGTTAATACCAATGTCTTGGTGGATAATGGTGAAACTGTAGTTTTGGG
TGGGATTTTTGAGCAAGAAACTAAAAGCAGCCAAACTAAAGTACCATTCTTAGGAGATATACCTTATCTTGGGCGTTTAT
TCCGTAAAGATCTAAAATCTGATAATAAACGTGAACTGCTAATCTTTGTGACACCAAGAATTGTTAATGACAGTCGTACG
AGAAATCATTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(379-461)

AMIN domain (AMIN)

(55-137)

Secretin and TonB N terminus short domain (STN)

(310-352)

Bacterial type II and III secretion system protein (Secretin)

(557-715)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Acinetobacter baumannii D1279779

73.003

100

0.733
  pilQ Acinetobacter baumannii strain A118

73.003

100

0.733
  comQ Acinetobacter baylyi ADP1

69.944

98.479

0.689
  pilQ Pseudomonas aeruginosa PAK

38.327

100

0.393

Multiple sequence alignment