Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TOL5_RS01735 Genome accession   NZ_AP024708
Coordinates   375934..377646 (-) Length   570 a.a.
NCBI ID   WP_035300190.1    Uniprot ID   A0A8I1AKW3
Organism   Acinetobacter sp. Tol 5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 370934..382646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOL5_RS01705 (TOL5_03270) - 370937..371260 (+) 324 WP_004718357.1 pyrimidine/purine nucleoside phosphorylase -
  TOL5_RS01710 (TOL5_03280) rlmB 371404..372156 (+) 753 WP_004826358.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  TOL5_RS01715 (TOL5_03290) - 372197..373117 (+) 921 WP_199934976.1 DMT family transporter -
  TOL5_RS01720 (TOL5_03300) coaE 373165..373764 (-) 600 WP_199934975.1 dephospho-CoA kinase -
  TOL5_RS22600 (TOL5_03310) pilD 373790..374671 (-) 882 WP_035300187.1 prepilin peptidase Machinery gene
  TOL5_RS01730 (TOL5_03320) pilC 374674..375906 (-) 1233 WP_042088565.1 type II secretion system F family protein Machinery gene
  TOL5_RS01735 (TOL5_03330) pilB 375934..377646 (-) 1713 WP_035300190.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TOL5_RS01740 (TOL5_03340) tpiA 377925..378719 (+) 795 WP_009584365.1 triose-phosphate isomerase -
  TOL5_RS01745 (TOL5_03350) secG 378729..379070 (+) 342 WP_004826367.1 preprotein translocase subunit SecG -
  TOL5_RS01770 (TOL5_03360) rimP 379865..380389 (+) 525 WP_004826368.1 ribosome maturation factor RimP -
  TOL5_RS01775 (TOL5_03370) nusA 380424..381908 (+) 1485 WP_004826370.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63142.64 Da        Isoelectric Point: 4.9084

>NTDB_id=87582 TOL5_RS01735 WP_035300190.1 375934..377646(-) (pilB) [Acinetobacter sp. Tol 5]
MSMQNGSPKFSGYIRRLVDEGFLSAQQMKDALTSAQKSKTHLVPYLIEKLAISPLLIAEMISSEFGEPIFDLDSFNPEML
PKDIVDAGLISKHGVIPLVQRGNILYVATSDPTNIEALDAIRFSCGLNIEPIIVEQHKLDKALAKHFTVEESFNFGDEDF
DVDIIATDENEPAELDEETAEEEAPIVKYVNKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRTITNPPLQLANRLAS
RLKVMSQMDISEKRIPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNLKAGLTFSVALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNHCKAPIEIPKQSLLELGF
TEQDLNHPDLQIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKLGFDNLRRSGLKKVMNGLTS
LQEINRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=87582 TOL5_RS01735 WP_035300190.1 375934..377646(-) (pilB) [Acinetobacter sp. Tol 5]
ATGTCTATGCAAAATGGTTCACCTAAGTTTTCGGGTTATATCCGTCGCTTAGTCGATGAAGGCTTTTTATCTGCTCAGCA
AATGAAAGATGCCTTAACAAGTGCTCAAAAAAGTAAAACCCATTTGGTGCCTTACCTGATTGAGAAATTAGCGATCTCAC
CATTGCTGATCGCTGAAATGATCTCCTCCGAGTTCGGTGAGCCTATTTTTGATCTAGACAGTTTTAATCCTGAAATGTTG
CCCAAGGACATTGTGGACGCCGGTTTAATCAGCAAACATGGGGTGATTCCATTGGTGCAACGCGGCAATATCTTATATGT
TGCCACCAGCGATCCCACCAATATCGAGGCACTTGATGCGATTCGTTTTAGTTGTGGTCTAAACATTGAGCCAATTATCG
TTGAACAACATAAATTAGACAAAGCTTTAGCCAAACATTTTACTGTAGAAGAAAGCTTTAATTTTGGCGATGAAGATTTT
GATGTTGATATTATTGCAACAGATGAAAATGAACCCGCCGAGCTTGATGAAGAAACTGCTGAAGAAGAAGCGCCGATTGT
TAAATATGTCAATAAACTATTGATTGATGCCATTCGAATGGGCGCCTCGGACTTACATTTTGAACCTTATGAAAAAAGTT
ATCGTGTTCGTTATCGTGTAGATGGTGTACTCAGAACTATCACCAATCCCCCTCTGCAATTGGCGAATCGTTTAGCATCC
CGTTTGAAAGTCATGTCTCAAATGGATATTTCAGAGAAACGTATTCCTCAAGATGGGCGGATCAAACTAAAACTGTCTAA
AACCAAAGCTATTGATTTCCGTGTCAACTCCCTGCCAACATTGTTTGGTGAAAAATTAGTTTTACGTATTCTTGATCCTT
CCAGTGCCATGTTAGGGATTGATGCTTTGGGCTATGAAGAAGACCAAAAAGCTTTATTTATGGAAGCCTTAGAAAAACCG
CAAGGCATGTTATTGATTACTGGTCCAACAGGTTCTGGTAAAACCGTATCGTTGTATACAGGCTTAAATATCCTCAATAC
CGAAAGTTCCAATATTTCAACCGCAGAAGACCCTGTCGAGATTAACTTGGAGGGGATTAACCAAGTAAACGTCAATCTAA
AAGCAGGATTGACCTTTTCGGTCGCGCTAAAATCATTTCTTCGTCAAGATCCAGATATTATCATGGTCGGGGAAATTCGA
GACCTTGAAACGGCTGAAATTGCAATCAAAGCAGCCCAAACAGGTCATATGGTGATGTCCACACTGCATACCAACAGCGC
ACCTGAAACCTTAACCCGTTTGCGCAATATGGGTGTGCCATCCTTCAATATTGCAACATCGGTCAATCTTGTGATTGCAC
AACGTCTTGCTCGGCGCTTATGCAACCACTGTAAAGCACCTATTGAAATTCCGAAACAAAGTTTGTTAGAACTGGGCTTT
ACTGAACAAGACTTAAACCATCCTGATTTACAGATTTTTCAGCCTGTGGGCTGTTCTGAATGTAGAGAAGGTTATAAAGG
ACGAGTCGGTATTTATGAGGTGATGAAAGTAACACCTGAAATTTCAAAAATTATCATGGAAGATGGCAACGCACTACAAA
TTGCAGCAGCATCTGAAAAATTGGGGTTTGATAATTTGCGACGTTCGGGTTTAAAAAAGGTTATGAATGGTTTAACGTCT
TTACAAGAAATCAACCGCGTAACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.245

100

0.805
  pilB Acinetobacter baylyi ADP1

75.088

100

0.751
  pilB Legionella pneumophila strain ERS1305867

51.215

100

0.518
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

48.654

97.719

0.475
  pilB Vibrio cholerae strain A1552

48.282

97.018

0.468
  pilB Vibrio campbellii strain DS40M4

49.712

91.404

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

34.91

100

0.375
  pilF Thermus thermophilus HB27

37.217

100

0.375

Multiple sequence alignment