Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   WHX55_RS26660 Genome accession   NZ_CP148752
Coordinates   5861285..5861707 (-) Length   140 a.a.
NCBI ID   WP_150757730.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain AB1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5856285..5866707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WHX55_RS26645 (WHX55_26615) - 5857236..5860193 (+) 2958 WP_150727062.1 DUF748 domain-containing protein -
  WHX55_RS26650 (WHX55_26620) - 5860193..5860489 (+) 297 WP_223446861.1 DUF2845 domain-containing protein -
  WHX55_RS26655 (WHX55_26625) - 5860629..5860985 (-) 357 WP_150757729.1 BON domain-containing protein -
  WHX55_RS26660 (WHX55_26630) pilA/pilAI 5861285..5861707 (-) 423 WP_150757730.1 pilin Machinery gene
  WHX55_RS26665 (WHX55_26635) pilC 5861935..5863152 (+) 1218 WP_151214145.1 type II secretion system F family protein Machinery gene
  WHX55_RS26670 (WHX55_26640) pilD 5863154..5864026 (+) 873 WP_353741595.1 A24 family peptidase Machinery gene
  WHX55_RS26675 (WHX55_26645) coaE 5864023..5864646 (+) 624 WP_353741596.1 dephospho-CoA kinase -
  WHX55_RS26680 (WHX55_26650) yacG 5864643..5864843 (+) 201 WP_007970539.1 DNA gyrase inhibitor YacG -
  WHX55_RS26685 (WHX55_26655) - 5864878..5865567 (-) 690 WP_150727090.1 energy-coupling factor ABC transporter permease -
  WHX55_RS26690 (WHX55_26660) - 5865639..5866571 (-) 933 WP_151214147.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14409.60 Da        Isoelectric Point: 7.6695

>NTDB_id=873528 WHX55_RS26660 WP_150757730.1 5861285..5861707(-) (pilA/pilAI) [Pseudomonas fluorescens strain AB1]
MNNQKGFTLIELLVVVAIIGILATLGLPMYSKYQARAKVTAALAELTAMKVPFEDVISQGETPTIANVAPAGETSTNHCA
LSVTATTPAGAGSIVCTLHNAPGVVQGKTVTLTRDEDKGWSCKSSVSKDYLPKGCSGSAA

Nucleotide


Download         Length: 423 bp        

>NTDB_id=873528 WHX55_RS26660 WP_150757730.1 5861285..5861707(-) (pilA/pilAI) [Pseudomonas fluorescens strain AB1]
ATGAACAATCAGAAAGGTTTTACCCTGATCGAGTTGCTCGTCGTGGTGGCGATCATCGGGATCCTGGCAACACTGGGTCT
GCCGATGTATTCCAAGTACCAGGCTCGGGCCAAGGTCACGGCGGCGCTGGCCGAACTGACTGCAATGAAGGTGCCGTTCG
AGGATGTGATCAGCCAGGGGGAAACCCCGACAATTGCCAACGTGGCGCCCGCTGGCGAGACATCGACCAACCATTGCGCC
CTGTCTGTGACGGCTACGACGCCGGCGGGAGCGGGATCAATCGTTTGTACGTTGCACAATGCCCCGGGGGTGGTCCAGGG
AAAAACCGTCACCCTGACACGGGACGAAGACAAAGGCTGGTCATGTAAAAGCAGCGTTTCCAAGGATTACTTGCCAAAAG
GGTGTTCCGGTTCTGCGGCATGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)

Pilin (bacterial filament) (Pilin)

(35-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.662

100

0.443
  pilA Acinetobacter baumannii strain A118

43.478

98.571

0.429
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.704

96.429

0.421
  pilA Pseudomonas aeruginosa PAK

37.748

100

0.407
  comP Acinetobacter baylyi ADP1

36.242

100

0.386
  pilA Vibrio cholerae C6706

37.241

100

0.386
  pilA Vibrio cholerae strain A1552

37.241

100

0.386
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.241

100

0.386