Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RCF98_RS11350 Genome accession   NZ_CP133218
Coordinates   2332065..2333312 (+) Length   415 a.a.
NCBI ID   WP_051543551.1    Uniprot ID   -
Organism   Thiothrix lacustris strain MK1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2327065..2338312
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF98_RS11335 (RCF98_11335) tviB 2328039..2329316 (+) 1278 WP_308893844.1 Vi polysaccharide biosynthesis UDP-N-acetylglucosamine C-6 dehydrogenase TviB -
  RCF98_RS11340 (RCF98_11340) tfpZ 2329334..2330101 (+) 768 WP_308893846.1 TfpX/TfpZ family type IV pilin accessory protein -
  RCF98_RS11345 (RCF98_11345) pilB 2330232..2331956 (+) 1725 WP_028490507.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RCF98_RS11350 (RCF98_11350) pilC 2332065..2333312 (+) 1248 WP_051543551.1 type II secretion system F family protein Machinery gene
  RCF98_RS11355 (RCF98_11355) pilD 2333326..2334198 (+) 873 WP_028490509.1 A24 family peptidase Machinery gene
  RCF98_RS11360 (RCF98_11360) - 2334208..2334639 (-) 432 WP_028490510.1 hypothetical protein -
  RCF98_RS11365 (RCF98_11365) ubiE 2334724..2335476 (+) 753 WP_308893848.1 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE -
  RCF98_RS11370 (RCF98_11370) opgC 2335422..2336609 (-) 1188 WP_028490512.1 OpgC domain-containing protein -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 45308.19 Da        Isoelectric Point: 10.2918

>NTDB_id=872560 RCF98_RS11350 WP_051543551.1 2332065..2333312(+) (pilC) [Thiothrix lacustris strain MK1]
MQSKTLKKSTQAKASPSASQAVYSWEGVNRNGVKMRGETQAVNPNWLRAELRRKGINPGRIYKKARPLFKPAIKPADIAQ
FARQLTTMMRSGVPMVQSLELMASGGDNVSVRDLIKKLTADIEGGASLGSALARHPKYFDKLFVNLVKAGEQAGTLETML
EKVASYKEKSEALKAKVKSAMTYPIIVVVAAVVVSAILLIWVIPQFKEVFSSFGADLPAFTLLVISFSDWLVANWWMPIL
VFVAIGFSFTQARQRSPAFDRFVDKASLKIPIMGNILNLSAVARFARTLATMFAAGVPLVEAMDSVAGATGNALYEEASR
RIQDDTARGVQLNTAMMTTQLFPNMMVQMTRIGEESGRLEEMLAKVADYYEAQVDALVDTLAKQIEPLIMAVLGGIVGSL
VIAMYLPIFKLGSVV

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=872560 RCF98_RS11350 WP_051543551.1 2332065..2333312(+) (pilC) [Thiothrix lacustris strain MK1]
ATGCAAAGCAAAACCTTGAAAAAATCAACACAGGCGAAAGCATCGCCCAGTGCATCCCAAGCTGTTTACAGCTGGGAAGG
CGTCAACCGTAATGGTGTGAAGATGCGTGGGGAAACCCAAGCGGTTAACCCCAACTGGTTACGTGCTGAACTGCGGCGCA
AAGGTATTAATCCAGGGCGTATCTATAAAAAAGCGAGACCCCTGTTCAAGCCTGCTATTAAACCAGCAGATATTGCCCAG
TTTGCCCGCCAGCTGACGACCATGATGCGCTCAGGTGTTCCAATGGTGCAGTCACTTGAGCTGATGGCATCAGGCGGGGA
TAATGTGTCGGTGCGTGACTTAATCAAGAAATTGACGGCGGATATTGAGGGAGGGGCTAGCCTCGGTAGTGCCTTAGCAC
GCCATCCCAAATATTTCGATAAGTTATTCGTCAATCTCGTTAAGGCTGGTGAGCAAGCGGGTACGTTGGAAACGATGCTT
GAAAAAGTAGCCAGTTATAAAGAAAAAAGTGAAGCGCTCAAAGCTAAGGTCAAAAGTGCGATGACCTACCCGATTATCGT
TGTTGTTGCCGCAGTGGTGGTGTCGGCCATCCTGTTAATCTGGGTTATTCCGCAGTTTAAGGAAGTGTTTAGCAGTTTTG
GCGCGGACTTGCCTGCCTTTACCTTATTGGTGATTTCCTTTTCAGACTGGTTAGTCGCTAATTGGTGGATGCCAATTTTG
GTCTTTGTTGCCATCGGATTTAGCTTTACTCAGGCAAGGCAGCGTTCTCCTGCCTTCGATCGTTTTGTGGATAAAGCCTC
GTTAAAGATACCGATCATGGGTAATATCCTGAATCTGTCGGCTGTTGCCCGTTTTGCTCGCACACTTGCCACCATGTTTG
CTGCTGGTGTCCCCTTGGTCGAGGCGATGGATTCCGTAGCAGGCGCAACCGGTAATGCGCTGTATGAGGAGGCTTCGCGT
CGCATTCAGGACGATACGGCACGCGGTGTACAGCTCAATACCGCGATGATGACCACACAATTATTCCCTAATATGATGGT
ACAAATGACTCGTATCGGTGAAGAATCTGGGCGACTGGAAGAAATGCTGGCGAAAGTCGCCGATTATTACGAAGCACAGG
TAGACGCCTTGGTGGATACCCTTGCTAAACAAATTGAACCGCTGATCATGGCGGTATTGGGTGGTATCGTCGGTAGTTTG
GTTATTGCCATGTACTTGCCCATCTTTAAACTTGGATCAGTCGTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(285-407)

Type II secretion system (T2SS), protein F (T2SSF)

(81-204)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.96

97.349

0.525
  pilC Acinetobacter baylyi ADP1

51.005

95.904

0.489
  pilC Legionella pneumophila strain ERS1305867

50.376

96.145

0.484
  pilC Acinetobacter baumannii D1279779

49.37

95.663

0.472
  pilG Neisseria meningitidis 44/76-A

43.325

95.663

0.414
  pilG Neisseria gonorrhoeae MS11

43.073

95.663

0.412
  pilC Vibrio cholerae strain A1552

39.706

98.313

0.39
  pilC Vibrio campbellii strain DS40M4

40.554

95.663

0.388