Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RCF98_RS11345 Genome accession   NZ_CP133218
Coordinates   2330232..2331956 (+) Length   574 a.a.
NCBI ID   WP_028490507.1    Uniprot ID   -
Organism   Thiothrix lacustris strain MK1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2325232..2336956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF98_RS11325 (RCF98_11325) - 2325530..2326969 (+) 1440 WP_308893842.1 MBOAT family O-acyltransferase -
  RCF98_RS11330 (RCF98_11330) - 2326944..2328005 (+) 1062 WP_028490505.1 hypothetical protein -
  RCF98_RS11335 (RCF98_11335) tviB 2328039..2329316 (+) 1278 WP_308893844.1 Vi polysaccharide biosynthesis UDP-N-acetylglucosamine C-6 dehydrogenase TviB -
  RCF98_RS11340 (RCF98_11340) tfpZ 2329334..2330101 (+) 768 WP_308893846.1 TfpX/TfpZ family type IV pilin accessory protein -
  RCF98_RS11345 (RCF98_11345) pilB 2330232..2331956 (+) 1725 WP_028490507.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RCF98_RS11350 (RCF98_11350) pilC 2332065..2333312 (+) 1248 WP_051543551.1 type II secretion system F family protein Machinery gene
  RCF98_RS11355 (RCF98_11355) pilD 2333326..2334198 (+) 873 WP_028490509.1 A24 family peptidase Machinery gene
  RCF98_RS11360 (RCF98_11360) - 2334208..2334639 (-) 432 WP_028490510.1 hypothetical protein -
  RCF98_RS11365 (RCF98_11365) ubiE 2334724..2335476 (+) 753 WP_308893848.1 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE -
  RCF98_RS11370 (RCF98_11370) opgC 2335422..2336609 (-) 1188 WP_028490512.1 OpgC domain-containing protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63182.26 Da        Isoelectric Point: 6.9130

>NTDB_id=872559 RCF98_RS11345 WP_028490507.1 2330232..2331956(+) (pilB) [Thiothrix lacustris strain MK1]
MIPMTSSIPLPGLARQLVVQDLLDKDLAAQAVQKARSEKVSLVEWLIRSRIVSTKDVAYAASLEYGLSMIDLDQINIPAP
VSILLSQDQMRKLNLIPLVQRGKNLIVGLADPTYLTNLDDVKFATGLIPEPVFVEFDKLQRFLRIDDGMMGHASAPVPGG
KSTASAAPAASAKEILLDVRDLKTKDDEDPAIIEFVDKLLAHGIQSKASDIHIEIFEKNMRIRYRIDGILQVVANPPNDS
AQQLISRIKVLARMNISERRVPQDGRIRFTVNDQKKIDFRVNTLPTLFGEKVVMRILDSSNATLGVEKLGFDPKQQHDFM
KALEKPDGMILVTGPTGSGKTVTLYTGLGILNTPEKNISTAEDPAEINMPGVNQVNVNAKVGLTFAEALRSFLRQDPDII
MVGEIRDLETAEISIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPPFNIASSVHLIIAQRLARRLCEKCKQPSDVPHEV
LLQMGFKQEELASLKLYEPVGCAECSRGYKGRVGIYQVMPISGDMGRMIMNGSNSMEIAEQSLKEGILDLRRSALNKVRQ
GITSLAELERVTSD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=872559 RCF98_RS11345 WP_028490507.1 2330232..2331956(+) (pilB) [Thiothrix lacustris strain MK1]
ATGATTCCTATGACATCTTCCATTCCTTTGCCGGGACTCGCCAGACAATTAGTCGTGCAAGACTTGCTCGACAAAGATCT
GGCAGCCCAAGCTGTGCAGAAAGCACGCTCGGAAAAAGTGTCGCTAGTCGAGTGGCTTATTCGCTCGCGTATCGTTTCTA
CCAAAGATGTCGCCTATGCTGCTAGCCTAGAATATGGGCTAAGCATGATCGACCTCGATCAAATCAATATTCCAGCACCC
GTGTCGATTCTGCTCAGTCAAGATCAGATGCGAAAACTCAATCTGATCCCCTTGGTGCAACGCGGCAAAAACCTAATCGT
GGGTTTAGCTGATCCAACGTATCTGACCAATTTAGATGATGTTAAATTTGCCACAGGGCTGATTCCTGAGCCAGTGTTTG
TGGAATTTGACAAGCTGCAACGTTTTCTACGCATTGACGATGGGATGATGGGGCACGCGAGTGCTCCGGTACCTGGTGGG
AAATCGACAGCGTCAGCTGCGCCCGCCGCTTCTGCTAAAGAAATTTTGCTCGATGTTCGCGATCTCAAAACCAAGGATGA
TGAAGACCCCGCCATCATTGAATTTGTCGATAAATTGCTTGCACACGGTATTCAGTCTAAAGCATCGGATATCCACATCG
AAATTTTCGAAAAAAACATGCGTATCCGTTACCGAATCGACGGCATTCTGCAAGTGGTTGCAAACCCACCCAATGACTCA
GCGCAACAACTGATTTCGCGCATCAAAGTATTGGCACGGATGAATATTTCCGAACGACGTGTACCACAAGACGGACGTAT
CCGTTTTACGGTGAATGATCAGAAAAAAATCGATTTCCGTGTTAATACACTGCCCACACTCTTCGGTGAAAAGGTGGTCA
TGCGGATACTCGATTCCTCCAATGCGACTTTGGGCGTGGAAAAACTGGGGTTTGACCCCAAACAACAGCATGATTTCATG
AAGGCACTGGAAAAACCGGATGGCATGATTCTGGTAACAGGCCCGACAGGTAGTGGTAAAACCGTTACCTTGTATACAGG
TTTGGGCATTCTCAATACCCCAGAGAAAAACATTTCTACTGCCGAGGATCCGGCTGAAATCAATATGCCGGGCGTTAACC
AAGTCAATGTGAATGCGAAAGTTGGCCTGACGTTTGCGGAAGCGTTGCGTTCCTTCCTGCGGCAAGACCCGGATATCATC
ATGGTGGGGGAGATCCGTGACCTAGAAACCGCTGAAATTTCCATAAAGGCAGCACAAACAGGTCACTTAGTCTTATCCAC
ATTACACACCAATAGCGCTCCAGAAACGCTGACACGTTTGATGAACATGGGCGTGCCGCCCTTCAATATCGCCTCTTCAG
TACACCTGATTATTGCTCAACGTCTGGCGCGGCGTTTGTGTGAAAAGTGCAAGCAACCTTCTGATGTACCCCATGAGGTG
TTGTTGCAAATGGGATTCAAACAAGAAGAGCTGGCTTCACTCAAGCTTTATGAGCCGGTGGGTTGTGCAGAATGTTCACG
TGGTTACAAGGGGCGGGTCGGTATTTATCAAGTCATGCCGATTTCAGGTGATATGGGACGGATGATCATGAATGGCAGTA
ACTCCATGGAGATTGCGGAACAATCACTCAAAGAAGGTATTCTGGATTTGCGTCGTTCTGCCCTAAATAAGGTTCGCCAA
GGTATTACCTCGCTGGCCGAATTGGAGCGTGTTACGTCCGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

50.94

100

0.519
  pilB Acinetobacter baylyi ADP1

50.868

100

0.51
  pilB Legionella pneumophila strain ERS1305867

49.828

100

0.503
  pilF Neisseria gonorrhoeae MS11

48.239

98.955

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

46.422

94.948

0.441
  pilB Vibrio cholerae strain A1552

45.688

94.948

0.434
  pilB Vibrio campbellii strain DS40M4

45.856

94.599

0.434
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.209

96.864

0.38
  pilF Thermus thermophilus HB27

37.993

97.213

0.369