Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RBH93_RS01970 Genome accession   NZ_CP133079
Coordinates   401048..402754 (-) Length   568 a.a.
NCBI ID   WP_307896095.1    Uniprot ID   -
Organism   Aeromonas veronii strain HS1906     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 396048..407754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBH93_RS01940 (RBH93_01940) mutT 396730..397143 (+) 414 WP_307896090.1 8-oxo-dGTP diphosphatase MutT -
  RBH93_RS01945 (RBH93_01945) yacG 397204..397398 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  RBH93_RS01950 (RBH93_01950) zapD 397408..398130 (-) 723 WP_024945431.1 cell division protein ZapD -
  RBH93_RS01955 (RBH93_01955) coaE 398168..398782 (-) 615 WP_307896092.1 dephospho-CoA kinase -
  RBH93_RS01960 (RBH93_01960) pilD 398796..399668 (-) 873 WP_307896093.1 A24 family peptidase Machinery gene
  RBH93_RS01965 (RBH93_01965) pilC 399691..400932 (-) 1242 WP_076361971.1 type II secretion system F family protein Machinery gene
  RBH93_RS01970 (RBH93_01970) pilB 401048..402754 (-) 1707 WP_307896095.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  RBH93_RS01975 (RBH93_01975) - 402758..403234 (-) 477 WP_307896097.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RBH93_RS01980 (RBH93_01980) nadC 403550..404425 (-) 876 WP_307896099.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RBH93_RS01985 (RBH93_01985) - 404431..404901 (-) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  RBH93_RS01990 (RBH93_01990) ampD 405048..405617 (+) 570 WP_307896101.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RBH93_RS01995 (RBH93_01995) pdhR 405896..406660 (+) 765 WP_159144446.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62467.85 Da        Isoelectric Point: 5.4342

>NTDB_id=871659 RBH93_RS01970 WP_307896095.1 401048..402754(-) (pilB) [Aeromonas veronii strain HS1906]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=871659 RBH93_RS01970 WP_307896095.1 401048..402754(-) (pilB) [Aeromonas veronii strain HS1906]
ATGACATCTAGCCCCAATAGCGGCCTGGCGTTAAGCTTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCGCAGCGTAAACCCTTTGTGACCTTCCTGATCGAAAATGAAATCCTCGACAGCAAGGCATTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTGCCCATCTATACCCAAGGTCACACCCTCTATATCGCCATGTCGGA
TCCCACCAATGTGTCTGCACTGGAGGATTTCGGCTTCAGCTTCGGGTTGCATACCGAGGCACTGCTGGTAGAGGAAAATA
AGCTCACCGCCGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAGCTAGAAGTGTCTGACGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCCGACGATGACGCGCCCATCGTCAA
ATACATCAACAAAATAATGATGGACGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGGTAGCAACCCCACCGGTCAATCTGGCCAACCGCTTCTCGGCACGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAACGGCGACTGCCCCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAATCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGGCAGAAGGCGCAATATCTGCGTGCTCTCTCGAAGCCTCAG
GGCATGATTCTGGTGACCGGCCCGACCGGTTCTGGCAAGACGGTGTCGCTCTACACCGGCCTGAACATTCTCAACACCAC
AGAAGTAAATATTTCGACCGCCGAAGACCCGGTGGAGATCAACCTGCCAGGGGTCAATCAGGTACAGGTCAATCCCAAGG
CGGGGCTCACCTTCGCCAGTGCCCTGCGCTCCTTTCTGCGCCAGGATCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAGACCGCTGAAATTGCCATCAAGGCCGCCCAGACAGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACTCTGATCATGGCTCAGC
GGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCGGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGGTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAGATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCTGGCCTTGAGAAGGCCCGCCTTGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.536

100

0.556
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.69

99.472

0.544
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.246

98.944

0.507
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375