Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RBH02_RS23885 Genome accession   NZ_CP132993
Coordinates   5150357..5151574 (+) Length   405 a.a.
NCBI ID   WP_003161763.1    Uniprot ID   A0A3P3S6C0
Organism   Pseudomonas aeruginosa strain SRMPA3860     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5148888..5159055 5150357..5151574 within 0
IScluster/Tn 5148888..5150050 5150357..5151574 flank 307


Gene organization within MGE regions


Location: 5148888..5159055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RBH02_RS23880 (RBH02_23885) - 5150048..5150353 (+) 306 Protein_4727 type IV-A pilus assembly ATPase PilB -
  RBH02_RS23885 (RBH02_23890) pilC 5150357..5151574 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  RBH02_RS23890 (RBH02_23895) pilD 5151575..5152447 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  RBH02_RS23895 (RBH02_23900) coaE 5152444..5153055 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  RBH02_RS23900 (RBH02_23905) yacG 5153052..5153252 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  RBH02_RS23905 (RBH02_23910) - 5153289..5153498 (-) 210 WP_003094660.1 hypothetical protein -
  RBH02_RS23910 (RBH02_23915) - 5153604..5154293 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  RBH02_RS23915 (RBH02_23920) - 5154290..5154760 (-) 471 WP_003103866.1 hypothetical protein -
  RBH02_RS23920 (RBH02_23925) - 5154757..5155182 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  RBH02_RS23925 (RBH02_23930) - 5155315..5155944 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  RBH02_RS23930 (RBH02_23935) - 5155941..5156390 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  RBH02_RS23935 (RBH02_23940) - 5156416..5156586 (+) 171 WP_003094672.1 DUF3094 family protein -
  RBH02_RS23940 (RBH02_23945) - 5156650..5157957 (+) 1308 WP_003112837.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43998.80 Da        Isoelectric Point: 9.8103

>NTDB_id=871167 RBH02_RS23885 WP_003161763.1 5150357..5151574(+) (pilC) [Pseudomonas aeruginosa strain SRMPA3860]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTQMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=871167 RBH02_RS23885 WP_003161763.1 5150357..5151574(+) (pilC) [Pseudomonas aeruginosa strain SRMPA3860]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTTGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCGGCCTTCACCCAAATGG
TCATCAATATCTCCAACGTGCTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCTATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAGTCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3P3S6C0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baylyi ADP1

61.275

100

0.617
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria gonorrhoeae MS11

46.287

99.753

0.462
  pilG Neisseria meningitidis 44/76-A

45.771

99.259

0.454
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.898

99.012

0.405
  pilC Thermus thermophilus HB27

38.155

99.012

0.378