Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RAB71_RS15990 Genome accession   NZ_CP132343
Coordinates   3814016..3815746 (-) Length   576 a.a.
NCBI ID   WP_029561990.1    Uniprot ID   A0A2P5Z0V7
Organism   Xanthomonas sacchari strain CFBP4641     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3809016..3820746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAB71_RS15975 (RAB71_15970) sucC 3809255..3810424 (-) 1170 WP_010342227.1 ADP-forming succinate--CoA ligase subunit beta -
  RAB71_RS15980 (RAB71_15975) - 3810766..3812379 (+) 1614 WP_010342228.1 PAS domain-containing sensor histidine kinase -
  RAB71_RS15985 (RAB71_15980) pilR 3812462..3813850 (+) 1389 WP_010342229.1 sigma-54 dependent transcriptional regulator Regulator
  RAB71_RS15990 (RAB71_15985) pilB 3814016..3815746 (-) 1731 WP_029561990.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RAB71_RS15995 (RAB71_15990) - 3815947..3816354 (-) 408 WP_010342231.1 pilin -
  RAB71_RS16000 (RAB71_15995) pilC 3816713..3817972 (+) 1260 WP_010342232.1 type II secretion system F family protein Machinery gene
  RAB71_RS16005 (RAB71_16000) - 3817979..3818842 (+) 864 WP_010342233.1 A24 family peptidase -
  RAB71_RS16010 (RAB71_16005) coaE 3818856..3819479 (+) 624 WP_010342234.1 dephospho-CoA kinase -
  RAB71_RS16015 (RAB71_16010) - 3819617..3820024 (-) 408 WP_010342235.1 RNA-binding S4 domain-containing protein -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62632.88 Da        Isoelectric Point: 5.4655

>NTDB_id=868300 RAB71_RS15990 WP_029561990.1 3814016..3815746(-) (pilB) [Xanthomonas sacchari strain CFBP4641]
MNAMTSNLVGITGIARRLVQDGVLEEAAARTAMAQAAEAKVPLPQWFSEKKLVTAAQLAAANAVEFGMPLMDVSVFDASQ
NAMKLVSEELLQKYQVLPLFKRGTRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDSLGSALGD
DEGMDNLDVGGGDEDMGGGGDTGIDAKGEDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKNKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPVQLPEK
ALLAEGFTEAEIAAGVQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIASAAQNIGVKDLRQSALLKA
RNGITSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=868300 RAB71_RS15990 WP_029561990.1 3814016..3815746(-) (pilB) [Xanthomonas sacchari strain CFBP4641]
ATGAACGCAATGACGAGCAACCTAGTCGGCATCACCGGGATCGCCCGCCGCCTCGTGCAGGACGGTGTGCTCGAGGAAGC
CGCGGCGCGGACCGCCATGGCCCAGGCGGCCGAGGCGAAGGTGCCGCTGCCGCAGTGGTTCTCCGAGAAGAAGCTGGTCA
CTGCCGCACAGCTGGCCGCCGCGAATGCGGTCGAATTCGGTATGCCGTTGATGGATGTCTCGGTTTTCGACGCCAGCCAG
AACGCGATGAAGCTGGTCAGCGAGGAGCTGCTGCAGAAGTACCAAGTGCTGCCGTTGTTCAAGCGTGGCACCCGCCTGTT
CGTCGGCATCAGCAACCCCACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGACGGTGGAGCCGATCC
TGGTCGACGAGGACCAGATCCGCCGGACCCTGGAGCAGTGGCAGGCCAGCAACGATTCCCTCGGCAGCGCGCTGGGCGAC
GACGAGGGCATGGACAACCTCGATGTCGGCGGCGGCGACGAGGACATGGGCGGCGGTGGCGACACCGGTATCGACGCCAA
GGGCGAGGACACCCCGGTCGTCAAGTTCGTGAACAAGGTGCTGGTCGATGCGATCCGCCGTGGCGCGTCCGACATCCACT
TCGAGCCCTACGAGGACGACTATCGGGTGCGCCTGCGCATCGACGGTCTGCTCAAGAGCGTGGCCAAGGCCCCGGTCAAG
CTCAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCCCAGCTGGACATCGCCGAGAAGCGGGTCCCGCAGGATGGTCG
CATCAAGCTCAACCTGTCCAAGAACAAGCAGATCGACTTCCGTGTCAGCACGCTGCCGACGCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCAGTGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAGCTGTTC
CTGGATGCGATCCACAAGCCGTATGGCATGGTCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGATACCCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATCCGGCTGCCCGGGGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
CACCCTGCACACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATTGCGCCCTACAACATCACCAGTT
CGGTGACCCTGGTCATCGCCCAGCGCCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGTCCGGTCCAGTTGCCCGAGAAG
GCCTTGCTGGCCGAAGGCTTCACCGAGGCGGAGATCGCCGCCGGCGTCCAGCTGTACGAGGCGGTCGGCTGCGACGAGTG
CACCGAGGGCTACAAGGGCCGCACCGGCATCTACCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGCTGCAGG
GCGGCAACGCCATGGACATCGCCAGCGCCGCGCAGAACATCGGCGTCAAGGACCTGCGCCAGTCGGCCCTGCTGAAGGCC
CGAAACGGCATCACCAGTCTGGCCGAGATCAATCGGGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2P5Z0V7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.72

99.306

0.543
  pilB Acinetobacter baumannii D1279779

54.737

98.958

0.542
  pilB Legionella pneumophila strain ERS1305867

51.318

98.785

0.507
  pilB Vibrio cholerae strain A1552

49.567

100

0.497
  pilF Neisseria gonorrhoeae MS11

49.296

98.611

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

45.798

100

0.464
  pilB Vibrio campbellii strain DS40M4

45.694

98.785

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

36.393

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.037

100

0.382
  pilF Thermus thermophilus HB27

39.619

91.146

0.361