Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O5O45_RS01575 Genome accession   NZ_CP132203
Coordinates   312838..314553 (-) Length   571 a.a.
NCBI ID   WP_305903555.1    Uniprot ID   -
Organism   Hahella sp. HNIBRBA332     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 307838..319553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O5O45_RS01545 (O5O45_01545) tsaA 307969..308685 (-) 717 WP_305903549.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  O5O45_RS01550 (O5O45_01550) - 308695..309744 (-) 1050 WP_305903550.1 hypothetical protein -
  O5O45_RS01555 (O5O45_01555) yacG 309795..310031 (-) 237 WP_305903551.1 DNA gyrase inhibitor YacG -
  O5O45_RS01560 (O5O45_01560) coaE 310021..310647 (-) 627 WP_305903552.1 dephospho-CoA kinase -
  O5O45_RS01565 (O5O45_01565) pilD 310667..311545 (-) 879 WP_305903553.1 A24 family peptidase Machinery gene
  O5O45_RS01570 (O5O45_01570) pilC 311614..312834 (-) 1221 WP_305903554.1 type II secretion system F family protein Machinery gene
  O5O45_RS01575 (O5O45_01575) pilB 312838..314553 (-) 1716 WP_305903555.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O5O45_RS01580 (O5O45_01580) pilA2 314872..315300 (+) 429 WP_305903556.1 pilin Machinery gene
  O5O45_RS01585 (O5O45_01585) - 315973..317931 (+) 1959 WP_305903557.1 tetratricopeptide repeat protein -
  O5O45_RS01590 (O5O45_01590) - 317946..318893 (+) 948 WP_305903558.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62589.54 Da        Isoelectric Point: 5.5905

>NTDB_id=867630 O5O45_RS01575 WP_305903555.1 312838..314553(-) (pilB) [Hahella sp. HNIBRBA332]
MNTQITPPLTGLARRFVQDGLLDESVAREAQQSAQKAKEPFVSHIVKRGLANSTQLAYAASAEFGVPLLDLSSIAIEQIP
DKIVDEKLIRKHHALPVFKRGNRLFLAVADPTNLQALDEIKFHTGLGTDAILVEEDKLSTFIDKYLESHEQPLGDLGDTD
LDDIDISAGDDVDADDDGGGAEQDDAPIVKYINKMLLDAIKGGASDIHFEPYEKTYRVRFRTDGILNEVSKPSIKLAAKL
SARLKVMSQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKLGIDALGYEEDQKKMYLDALA
QPQGMILVTGPTGSGKTVSLYTGLNILNTTERNISTAEDPVEINLEGINQVNVNTRVGLDFSSALRAFLRQDPDVVMVGE
IRDLDTASIAIKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPAFNIATSVSLIIAQRLARRLCPECKQVITVPTDVLKAE
GFTDQQIETGFALHTPKGCGKCKDGYKGRVGIYEVVRITPAMQELIMNEASSIDLAKQAQKEGFRNLRQSALRKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=867630 O5O45_RS01575 WP_305903555.1 312838..314553(-) (pilB) [Hahella sp. HNIBRBA332]
ATGAACACTCAGATCACTCCTCCCCTTACAGGATTGGCTCGACGCTTTGTGCAAGATGGCTTGCTAGATGAAAGCGTCGC
AAGAGAAGCCCAGCAAAGCGCACAAAAAGCAAAAGAACCTTTCGTAAGCCATATCGTAAAACGAGGGCTTGCTAATTCAA
CTCAATTGGCTTACGCAGCTTCAGCTGAGTTTGGCGTCCCTCTGCTGGACCTTTCCAGCATAGCAATTGAACAGATACCG
GACAAAATAGTTGATGAAAAGCTGATCAGAAAGCATCACGCACTGCCCGTATTCAAGCGGGGGAATCGACTATTCCTTGC
TGTGGCAGACCCAACCAACTTACAAGCGCTTGACGAAATCAAGTTTCACACGGGCCTAGGTACCGACGCCATACTTGTAG
AAGAAGACAAGCTATCTACCTTTATTGATAAATATTTGGAATCCCATGAACAGCCTCTAGGGGACCTGGGAGACACTGAC
CTAGACGATATTGATATATCAGCAGGCGATGATGTTGATGCAGATGATGATGGAGGAGGCGCAGAACAAGATGACGCACC
TATTGTTAAATACATCAATAAAATGCTGTTGGATGCTATTAAGGGCGGGGCCTCTGATATCCATTTTGAGCCTTACGAGA
AGACCTATCGAGTGAGATTTAGGACAGATGGCATATTGAACGAAGTTTCTAAGCCTTCTATAAAATTAGCAGCCAAACTA
TCGGCCCGACTGAAAGTTATGTCACAGCTTGATATATCTGAACGGCGTGTTCCGCAAGACGGACGCATAAAAATGAAACT
CTCAAAAACCAAAGCAATTGACTTCCGTGTAAACACCTTGCCCACATTATGGGGTGAAAAAATTGTTTTACGGATTCTTG
ATCCTAGTAGCGCCAAACTTGGAATAGACGCGCTAGGGTACGAAGAAGACCAGAAAAAGATGTACTTGGATGCGCTTGCC
CAACCACAAGGAATGATTCTAGTTACAGGGCCAACAGGAAGTGGAAAGACAGTCTCTTTGTATACAGGCTTAAATATCCT
AAACACTACTGAACGTAACATTTCAACCGCAGAAGACCCTGTCGAGATAAACCTTGAAGGTATAAACCAAGTTAATGTCA
ATACACGGGTTGGCTTAGACTTTTCTTCTGCCCTAAGAGCTTTTTTAAGGCAAGACCCCGATGTAGTGATGGTGGGAGAA
ATTCGTGATTTGGATACAGCCTCCATTGCCATAAAGGCTGCTCAGACTGGCCACTTAGTTCTCTCCACCCTACACACAAA
CAGCGCCCCCGAAACACTAACTCGGCTCCGCAATATGGGCGTACCGGCTTTCAACATTGCCACGTCTGTGAGTTTAATTA
TTGCACAACGACTTGCGAGGCGACTTTGTCCTGAATGCAAACAGGTTATTACTGTCCCCACAGATGTGCTAAAGGCGGAG
GGTTTCACAGACCAACAAATTGAAACCGGCTTTGCATTGCACACCCCCAAGGGCTGTGGTAAATGCAAGGATGGTTACAA
AGGGCGTGTTGGCATCTATGAAGTGGTGAGGATAACTCCTGCAATGCAAGAATTAATTATGAATGAGGCTAGCTCGATAG
ATTTGGCAAAACAGGCGCAAAAAGAAGGCTTTAGAAATCTTAGGCAGTCAGCGCTAAGAAAAGTGATTGAGGGCGTAACT
AGCCTAGAAGAAGCAAACCGTGTAACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.348

100

0.567
  pilB Acinetobacter baumannii D1279779

57.143

99.299

0.567
  pilB Legionella pneumophila strain ERS1305867

56

100

0.564
  pilB Vibrio parahaemolyticus RIMD 2210633

52.679

98.074

0.517
  pilB Vibrio campbellii strain DS40M4

51.053

99.825

0.51
  pilB Vibrio cholerae strain A1552

54.028

89.142

0.482
  pilF Neisseria gonorrhoeae MS11

47.257

98.949

0.468
  pilB/pilB1 Synechocystis sp. PCC 6803

39.162

96.147

0.377