Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q9G97_RS00385 Genome accession   NZ_CP132194
Coordinates   87366..89075 (-) Length   569 a.a.
NCBI ID   WP_305899275.1    Uniprot ID   -
Organism   Psychrobacter sp. M13     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 82366..94075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9G97_RS00360 (Q9G97_00360) - 82962..83297 (+) 336 WP_201570831.1 pyrimidine/purine nucleoside phosphorylase -
  Q9G97_RS00365 (Q9G97_00365) rlmB 83491..84255 (+) 765 WP_201570832.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  Q9G97_RS00370 (Q9G97_00370) coaE 84224..84928 (-) 705 WP_305899273.1 dephospho-CoA kinase -
  Q9G97_RS00375 (Q9G97_00375) pilD 85136..86035 (-) 900 WP_305899274.1 A24 family peptidase Machinery gene
  Q9G97_RS00380 (Q9G97_00380) pilC 86043..87266 (-) 1224 WP_201570835.1 type II secretion system F family protein Machinery gene
  Q9G97_RS00385 (Q9G97_00385) pilB 87366..89075 (-) 1710 WP_305899275.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q9G97_RS00390 (Q9G97_00390) tpiA 89402..90214 (+) 813 WP_305899276.1 triose-phosphate isomerase -
  Q9G97_RS00395 (Q9G97_00395) secG 90461..90769 (+) 309 WP_025652589.1 preprotein translocase subunit SecG -
  Q9G97_RS00410 (Q9G97_00410) rimP 91703..92218 (+) 516 WP_305899277.1 ribosome maturation factor RimP -
  Q9G97_RS00415 (Q9G97_00415) nusA 92316..93800 (+) 1485 WP_201570847.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62668.97 Da        Isoelectric Point: 5.2314

>NTDB_id=867538 Q9G97_RS00385 WP_305899275.1 87366..89075(-) (pilB) [Psychrobacter sp. M13]
MSTVISKFGGLAEQLVSAGLVSDKDMRSAQTEAQQQQVGLVAYLVGNKLADAYKLALLLSESFGDPLFDLDVLNNSVIPK
DLVDEKIVRKFNALPIFKRGQRLFVALSDPTRIDAIDALAFNSRLSIEAVLVEENKLRRRIDSVYADTMQSFSSFADSDF
NVDFDEGGESEVETRLNDSVDEAPVVKFVNKMLVEAIRMGASDLHFEPYEKSYRVRFRVDGVMQKMANPPVQLANKIAAR
LKVMSQMDISERRVPQDGRIKLKLSKSRAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPDQKELFLTALSKPQ
GMILITGPTGSGKTVSLYTGINILNTGETNISTAEDPVEINLEGINQVNVNSKVGLTFANALKSFLRQDPDIVMVGEIRD
LETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLKNMGVASFNIATSVNLVIAQRLARRLCNNCKKPIDVPRISLVELGFS
DEQLDDSGNVIYGPIGCSECREGYKGRIGIYEVLRVTNDISRIIMEDGNAIDIKDAALKNGFRDLRHSGILKVLQGMTSI
QEMMRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=867538 Q9G97_RS00385 WP_305899275.1 87366..89075(-) (pilB) [Psychrobacter sp. M13]
ATGTCTACTGTTATTAGTAAATTTGGCGGCTTAGCAGAGCAGTTGGTCAGTGCAGGACTGGTCAGTGATAAAGATATGCG
CAGTGCTCAAACTGAGGCGCAGCAGCAACAGGTAGGTCTAGTCGCTTATTTGGTTGGCAACAAATTGGCTGATGCTTATA
AACTAGCTTTATTGCTGTCTGAATCTTTTGGCGATCCTCTGTTCGATCTCGATGTGCTTAATAATAGTGTAATTCCTAAA
GATCTAGTCGATGAAAAAATCGTTCGTAAATTTAACGCTTTGCCGATATTTAAGCGGGGTCAACGTCTTTTTGTGGCTTT
GAGTGATCCTACGCGTATCGACGCGATTGATGCACTAGCGTTTAACTCACGTCTATCTATTGAGGCAGTACTGGTCGAGG
AGAACAAGCTAAGAAGACGTATCGATAGTGTTTACGCGGATACAATGCAAAGCTTCAGTAGCTTCGCTGATAGCGATTTC
AACGTTGATTTTGATGAAGGGGGTGAAAGTGAGGTTGAAACCCGCCTTAATGATAGCGTTGATGAAGCGCCAGTAGTAAA
ATTTGTCAATAAGATGCTGGTTGAAGCTATTCGTATGGGTGCTTCAGATTTGCACTTCGAGCCGTATGAGAAGAGCTATC
GCGTACGCTTTCGAGTCGATGGTGTTATGCAAAAAATGGCCAATCCACCGGTACAACTGGCTAATAAGATTGCCGCTCGT
CTAAAGGTGATGTCACAAATGGATATCTCTGAGCGCCGCGTGCCGCAAGATGGACGTATCAAGCTCAAGCTCTCCAAAAG
CAGAGCTATCGATTTTCGAGTGAACTCATTGCCTACGCTGTTTGGTGAAAAAATCGTCCTGCGTATTTTAGATCCTTCAT
CAGCCATGCTAGGTATTGATGCGCTAGGCTATGAGCCTGATCAAAAAGAGCTGTTCTTGACCGCTTTAAGTAAGCCACAA
GGGATGATTCTGATTACAGGGCCTACTGGTTCTGGTAAAACAGTATCGTTATATACAGGTATTAATATCCTGAATACGGG
CGAGACTAATATTTCAACTGCAGAGGATCCAGTCGAGATCAACCTAGAGGGCATCAATCAGGTCAACGTTAATTCTAAGG
TAGGACTAACTTTTGCCAATGCACTCAAGTCATTCTTACGTCAAGATCCTGATATTGTGATGGTTGGTGAGATTCGTGAT
TTAGAAACCGCTGAGATCGCTGTTAAAGCAGCACAAACCGGTCACTTAGTATTATCGACGTTACATACTAACTCGGCTCC
CGAGACCTTGACTCGCCTCAAAAATATGGGCGTAGCTTCGTTTAACATTGCAACTTCAGTGAACTTAGTTATCGCTCAAC
GACTGGCTAGACGTTTATGCAATAACTGCAAAAAGCCTATCGATGTTCCTCGAATCAGTCTAGTAGAGCTGGGCTTTAGT
GATGAACAACTAGACGATTCAGGCAATGTCATCTACGGGCCTATAGGTTGTAGTGAATGCCGCGAAGGATATAAAGGCCG
AATCGGTATTTATGAGGTGCTAAGAGTAACTAATGATATCTCTCGTATCATTATGGAAGATGGTAATGCAATCGATATAA
AAGATGCGGCACTCAAAAACGGCTTTCGTGATTTGCGCCACTCAGGTATCTTAAAAGTATTGCAGGGCATGACTAGTATT
CAAGAGATGATGCGCGTGACTTCTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

65.957

99.121

0.654
  pilB Acinetobacter baylyi ADP1

62.61

99.649

0.624
  pilB Legionella pneumophila strain ERS1305867

50.353

99.473

0.501
  pilF Neisseria gonorrhoeae MS11

48.404

99.121

0.48
  pilB Vibrio campbellii strain DS40M4

47.857

98.418

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

50.379

92.794

0.467
  pilB Vibrio cholerae strain A1552

50.386

91.037

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.667

100

0.387
  pilB/pilB1 Synechocystis sp. PCC 6803

34.35

100

0.376
  pilF Thermus thermophilus HB27

37.037

99.649

0.369