Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K5632_RS02280 Genome accession   NZ_AP024615
Coordinates   487056..488762 (-) Length   568 a.a.
NCBI ID   WP_115389372.1    Uniprot ID   A0A379Z7V7
Organism   Shewanella algae strain TUM17383     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 482056..493762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5632_RS02250 (TUM17383_04280) mutT 482641..483039 (-) 399 WP_221055728.1 8-oxo-dGTP diphosphatase MutT -
  K5632_RS02255 (TUM17383_04290) yacG 483039..483251 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  K5632_RS02260 (TUM17383_04300) zapD 483296..484036 (-) 741 WP_028779562.1 cell division protein ZapD -
  K5632_RS02265 (TUM17383_04310) coaE 484033..484644 (-) 612 WP_025012056.1 dephospho-CoA kinase -
  K5632_RS02270 (TUM17383_04320) pilD 484645..485559 (-) 915 WP_221055729.1 A24 family peptidase Machinery gene
  K5632_RS02275 pilC 485630..486898 (-) 1269 WP_146509491.1 type II secretion system F family protein Machinery gene
  K5632_RS02280 (TUM17383_04340) pilB 487056..488762 (-) 1707 WP_115389372.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5632_RS02285 (TUM17383_04350) - 488826..489257 (-) 432 WP_221055730.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K5632_RS02290 (TUM17383_04360) nadC 490128..490988 (-) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5632_RS02295 (TUM17383_04370) ampD 491147..491725 (+) 579 WP_221055731.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5632_RS02300 (TUM17383_04380) ampE 491779..492630 (+) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  K5632_RS02305 (TUM17383_04390) pdhR 492905..493657 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63229.36 Da        Isoelectric Point: 5.3575

>NTDB_id=86599 K5632_RS02280 WP_115389372.1 487056..488762(-) (pilB) [Shewanella algae strain TUM17383]
MPTTSLHLGLSTLFLRRGLISEEQLSETVTQSRKHKQSLVTTLVTSRLLSARTIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLELDGLDEES
LAGIEVTDTDKRQQEENEGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINTKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEEIPEHELLQLGFS
QQQIDTGFTPYKPCGCELCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAATAKQQGMRDLRLSGLLKVIQGVTSIA
EVNRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=86599 K5632_RS02280 WP_115389372.1 487056..488762(-) (pilB) [Shewanella algae strain TUM17383]
ATGCCAACCACGAGCCTCCACCTTGGTTTATCAACCCTTTTCTTAAGACGGGGTTTAATCAGTGAAGAACAGTTATCTGA
AACGGTAACTCAGTCTCGTAAGCATAAACAATCGCTGGTAACCACTTTGGTGACCAGTAGGCTTTTGTCTGCCCGTACCA
TTGCCGAGCTCTGCTATGAAGAATACGGCACTCCACTGCTGGATCTCAATGAGTTTGATTTAAGCAGTATTCCGGAAGAG
TTTCTCAATAAAAAACTAATCGATAAGCATAGATGCCTGCCGCTATTTAAACGTGGTAATCGCCTCTATATCGCCACTTC
AGACCCCACCAATATCGCTGCACTGGAGGACTTCCAATTTAGCGCCGGCCTGCACGCGGAAGCGATACTGGTAGAGGAAG
ATAAGCTCAGTAAAGCGTTGGAAAAAGTACTCGAAGAAGACCTCAGCTCTTTAGAGCTAGATGGTCTCGATGAGGAATCC
CTCGCCGGTATCGAAGTCACAGATACCGACAAACGCCAACAGGAAGAGAATGAAGGCAAAGACGATGCGCCTATCGTCAT
CTATATCAATAAGATTTTGACCGATGCCATCCGCAAGGGCGCATCGGATCTGCATTTCGAGCCCTACGAGAAACGCTACC
GCATCCGTTTTCGTATTGATGGCATCCTCCACGAGGTATCTGAGCCACCAATTTCCCTCTCAGGCCGTATATCGGCCCGC
TTGAAAGTGATGTCCAAATTGGATATCGCCGAGCGCCGGGTACCTCAGGATGGCCGCATCAAAATGAAGCTGTCCCGCAA
CAAGAGTATCGATTTCCGGGTCAGTACCCTGCCCACCCTCTGGGGTGAAAAGATAGTGATGCGGATCCTCGACTCCTCTT
CCGCACAATTAGGAATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTATCTGGAGATGCTGGCAAGGCCTCAG
GGAATGATTCTGGTAACCGGCCCTACCGGCTCGGGCAAAACCGTATCCCTTTATACTGGCCTCAATATCCTCAATACCGA
AGAGCGCAATATCAGCACCGCTGAAGACCCAGTGGAGATCAACCTGGAAGGGGTCAACCAGGTGCATATCAACACCAAGG
CAGGGTTAACCTTCGCCTCGGCGCTGCGCTCCTTTTTGCGTCAGGACCCTGATGTGGTGATGGTGGGGGAAATTCGCGAC
CTGGAAACCGCCGAAATCGCCATCAAGGCGGCGCAAACCGGCCACTTGGTACTCTCAACCCTGCACACCAATTCATCGGC
TGAAACCCTTACCCGCCTGCTTAATATGGGTGTTCCAGGTTATAACATTGCCAGTTCTGTCAATCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTATGCCCCGAATGCCGCCAACCGGAAGAGATCCCCGAGCATGAACTGCTGCAACTGGGATTTAGT
CAGCAGCAGATAGACACAGGCTTTACCCCATATAAGCCCTGCGGCTGCGAGCTCTGCTCCGGTGGCTACAAGGGCCGGGT
CGGTATCTATGAGGTGATGAAGATGTCCGACGAAATCGCCAGAACCATTATGGAGGGCGGTAACTCACTGCAGATTGCCG
CCACTGCCAAACAGCAGGGGATGCGCGACCTGCGCCTATCAGGCTTACTTAAAGTTATTCAAGGCGTTACCAGTATTGCC
GAAGTCAATCGGGTCACCAGCTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A379Z7V7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.97

99.12

0.585
  pilB Acinetobacter baylyi ADP1

58.824

98.768

0.581
  pilB Legionella pneumophila strain ERS1305867

56.007

99.648

0.558
  pilB Vibrio parahaemolyticus RIMD 2210633

52.993

100

0.53
  pilB Vibrio cholerae strain A1552

53.442

97.183

0.519
  pilB Vibrio campbellii strain DS40M4

51.865

99.12

0.514
  pilF Neisseria gonorrhoeae MS11

47.788

99.472

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

37.043

100

0.393
  pilF Thermus thermophilus HB27

38.23

99.472

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.779

98.063

0.38

Multiple sequence alignment