Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   K5632_RS02270 Genome accession   NZ_AP024615
Coordinates   484645..485559 (-) Length   304 a.a.
NCBI ID   WP_221055729.1    Uniprot ID   -
Organism   Shewanella algae strain TUM17383     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 479645..490559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5632_RS02250 (TUM17383_04280) mutT 482641..483039 (-) 399 WP_221055728.1 8-oxo-dGTP diphosphatase MutT -
  K5632_RS02255 (TUM17383_04290) yacG 483039..483251 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  K5632_RS02260 (TUM17383_04300) zapD 483296..484036 (-) 741 WP_028779562.1 cell division protein ZapD -
  K5632_RS02265 (TUM17383_04310) coaE 484033..484644 (-) 612 WP_025012056.1 dephospho-CoA kinase -
  K5632_RS02270 (TUM17383_04320) pilD 484645..485559 (-) 915 WP_221055729.1 A24 family peptidase Machinery gene
  K5632_RS02275 pilC 485630..486898 (-) 1269 WP_146509491.1 type II secretion system F family protein Machinery gene
  K5632_RS02280 (TUM17383_04340) pilB 487056..488762 (-) 1707 WP_115389372.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5632_RS02285 (TUM17383_04350) - 488826..489257 (-) 432 WP_221055730.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 33881.29 Da        Isoelectric Point: 7.2147

>NTDB_id=86597 K5632_RS02270 WP_221055729.1 484645..485559(-) (pilD) [Shewanella algae strain TUM17383]
MSEFIAVLSHNQWLFLVLCFLFASVIGSFLNVVIHRLPVMMKREWQQECNQYLQEYQPEFAKQLGEAKLNAPIDNYPAKY
NLLLPKSACPKCGAHIKPWHNLPIIGYLLLGGKCASCNTAISVRYPIFELLTALLISTLGWYFGPTLQFVYATILTFGLI
ALSGIDLDEMLLPDQITLPLLWLGLLVNISGTFTNLTDATIGAAAGYLSLWSVYWLFKLLTGKEGMGYGDFKLMALFGAW
LGWQMLPLIILLSSLVGALVGILLILFKNLNRGNPIPFGPYIAIAGWVALVWGQQIVDWYLGTL

Nucleotide


Download         Length: 915 bp        

>NTDB_id=86597 K5632_RS02270 WP_221055729.1 484645..485559(-) (pilD) [Shewanella algae strain TUM17383]
ATGTCAGAATTTATTGCCGTATTGAGCCACAACCAGTGGCTCTTTCTTGTTCTCTGTTTTTTGTTTGCCTCAGTAATAGG
CAGCTTTCTCAATGTGGTGATCCACCGCCTGCCGGTAATGATGAAGCGCGAATGGCAGCAGGAATGCAATCAATACCTGC
AGGAATATCAACCCGAATTCGCCAAACAGCTGGGTGAAGCCAAGCTTAATGCACCGATAGATAACTACCCGGCCAAATAC
AATCTGTTACTGCCGAAGTCTGCCTGCCCCAAGTGTGGCGCCCATATCAAACCCTGGCACAACCTCCCCATAATCGGCTA
TTTGTTGCTTGGCGGTAAATGCGCCTCCTGCAACACAGCTATTTCAGTGAGATACCCGATATTTGAGCTACTGACCGCCC
TGCTTATCAGTACTCTCGGTTGGTATTTTGGCCCGACACTGCAGTTTGTTTACGCCACTATTCTCACCTTTGGATTGATA
GCCCTCAGCGGGATAGATTTGGATGAAATGCTGCTGCCGGATCAGATTACCCTGCCGCTACTGTGGCTTGGTTTACTGGT
TAATATCTCAGGCACCTTTACTAATTTGACCGATGCCACCATAGGGGCCGCTGCCGGCTATTTAAGCCTCTGGTCGGTCT
ACTGGCTATTTAAACTGCTCACCGGCAAGGAAGGCATGGGCTATGGCGATTTCAAGCTGATGGCACTGTTTGGCGCCTGG
CTTGGCTGGCAGATGTTACCTCTAATTATTCTGCTCTCTTCTCTGGTCGGAGCCCTAGTAGGCATTTTACTTATTCTGTT
CAAGAACCTTAATCGGGGTAACCCTATCCCCTTTGGCCCTTATATTGCTATCGCAGGTTGGGTCGCTTTGGTGTGGGGGC
AACAGATTGTCGACTGGTATTTGGGAACACTCTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-143)

Type IV leader peptidase family (Peptidase_A24)

(154-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

48.837

99.013

0.484
  pilD Acinetobacter nosocomialis M2

48.344

99.342

0.48
  pilD Vibrio cholerae strain A1552

49.658

96.053

0.477
  pilD Acinetobacter baumannii D1279779

48.013

99.342

0.477
  pilD Neisseria gonorrhoeae MS11

45.552

92.434

0.421

Multiple sequence alignment