Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q7586_RS21990 Genome accession   NZ_CP131791
Coordinates   4705009..4706712 (-) Length   567 a.a.
NCBI ID   WP_033944805.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2014S05-249     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4700009..4711712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7586_RS21950 (Q7586_21955) - 4700178..4700603 (+) 426 WP_016562341.1 GNAT family N-acetyltransferase -
  Q7586_RS21955 (Q7586_21960) - 4700600..4701070 (+) 471 WP_003094664.1 hypothetical protein -
  Q7586_RS21960 (Q7586_21965) - 4701067..4701756 (+) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  Q7586_RS21965 (Q7586_21970) - 4701862..4702071 (+) 210 WP_003094660.1 hypothetical protein -
  Q7586_RS21970 (Q7586_21975) yacG 4702108..4702308 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  Q7586_RS21975 (Q7586_21980) coaE 4702305..4702916 (-) 612 WP_003112838.1 dephospho-CoA kinase -
  Q7586_RS21980 (Q7586_21985) pilD 4702913..4703785 (-) 873 WP_033944809.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  Q7586_RS21985 (Q7586_21990) pilC 4703788..4705005 (-) 1218 WP_033944807.1 type II secretion system F family protein Machinery gene
  Q7586_RS21990 (Q7586_21995) pilB 4705009..4706712 (-) 1704 WP_033944805.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q7586_RS21995 (Q7586_22000) pilE 4706939..4707475 (+) 537 WP_025921208.1 pilin Machinery gene
  Q7586_RS22000 (Q7586_22005) tfpZ 4707540..4708277 (+) 738 WP_023100229.1 TfpX/TfpZ family type IV pilin accessory protein -
  Q7586_RS22010 (Q7586_22015) nadC 4708511..4709359 (-) 849 WP_003125200.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62622.59 Da        Isoelectric Point: 5.5586

>NTDB_id=865183 Q7586_RS21990 WP_033944805.1 4705009..4706712(-) (pilB) [Pseudomonas aeruginosa strain 2014S05-249]
MNDTIQLSGLARQLVLHELLDEKAAQQAQLQAQRNKLSLVTYLVQSKLVKGQALTELAADQFGIAYCDLNSLDRDSLPKD
LISEKLVRQHRIVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENVTDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRASGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=865183 Q7586_RS21990 WP_033944805.1 4705009..4706712(-) (pilB) [Pseudomonas aeruginosa strain 2014S05-249]
ATGAACGACACCATCCAGCTCAGCGGCCTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAAGCAGCTCAACA
GGCCCAGTTGCAAGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAGAGCAAGCTAGTGAAAGGACAGGCCT
TGACCGAACTGGCGGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGATCGGGACAGCCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGGATAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGTCACTGACAGCCTTGCAGGCCTGGATGACGTGGATCTTGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTATTGGATGCGATCAGAGGCGGCTCCTCTGATCTGCATTTCGAGCCCTATGAAAAGATTTACC
GTGTACGTTTCCGTACCGATGGCATGCTCCATGAGGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGA
CTCAAGGTAATGGCAGGCCTGGACATTTCCGAACGGCGTAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACTAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGGTTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGATCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAAC
AGGCCCGCAAAGAAGGCTTCAACGATCTACGCGCTTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.07

100

0.584
  pilB Acinetobacter baylyi ADP1

58.053

99.647

0.578
  pilB Legionella pneumophila strain ERS1305867

54.93

100

0.55
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.763

95.062

0.492
  pilB Vibrio cholerae strain A1552

50.545

97.002

0.49
  pilB Vibrio campbellii strain DS40M4

50.832

95.414

0.485
  pilF Thermus thermophilus HB27

38.407

99.647

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40

93.474

0.374