Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   Q7586_RS21995 Genome accession   NZ_CP131791
Coordinates   4706939..4707475 (+) Length   178 a.a.
NCBI ID   WP_025921208.1    Uniprot ID   Q8KQ36
Organism   Pseudomonas aeruginosa strain 2014S05-249     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4701939..4712475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7586_RS21970 (Q7586_21975) yacG 4702108..4702308 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  Q7586_RS21975 (Q7586_21980) coaE 4702305..4702916 (-) 612 WP_003112838.1 dephospho-CoA kinase -
  Q7586_RS21980 (Q7586_21985) pilD 4702913..4703785 (-) 873 WP_033944809.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  Q7586_RS21985 (Q7586_21990) pilC 4703788..4705005 (-) 1218 WP_033944807.1 type II secretion system F family protein Machinery gene
  Q7586_RS21990 (Q7586_21995) pilB 4705009..4706712 (-) 1704 WP_033944805.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q7586_RS21995 (Q7586_22000) pilE 4706939..4707475 (+) 537 WP_025921208.1 pilin Machinery gene
  Q7586_RS22000 (Q7586_22005) tfpZ 4707540..4708277 (+) 738 WP_023100229.1 TfpX/TfpZ family type IV pilin accessory protein -
  Q7586_RS22010 (Q7586_22015) nadC 4708511..4709359 (-) 849 WP_003125200.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Q7586_RS22015 (Q7586_22020) - 4709645..4711900 (+) 2256 WP_003104913.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 178 a.a.        Molecular weight: 18322.83 Da        Isoelectric Point: 4.9984

>NTDB_id=865184 Q7586_RS21995 WP_025921208.1 4706939..4707475(+) (pilE) [Pseudomonas aeruginosa strain 2014S05-249]
MKAQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRTRVSEGLVLAEPAKLMISTDGSASTADLTRATTTWNQQSNNLGA
SSKYVTSVLMDAGNTGVITITYVADQVGLPTAGNTLILSPYINDGNTRTALATAVAAGTRGTIDWACTSASNATATAQGF
TGMAAGSVPQEFAPAQCR

Nucleotide


Download         Length: 537 bp        

>NTDB_id=865184 Q7586_RS21995 WP_025921208.1 4706939..4707475(+) (pilE) [Pseudomonas aeruginosa strain 2014S05-249]
ATGAAAGCTCAAAAAGGCTTCACTTTGATCGAGCTGATGATCGTTGTAGCGATCATCGGTATTCTGGCTGCGATTGCTCT
GCCGGCCTATCAGGACTACACCGTTCGTACCCGTGTCAGTGAAGGTCTGGTTCTGGCTGAGCCCGCCAAACTGATGATCT
CCACGGATGGAAGTGCCTCTACTGCTGATCTGACTCGCGCAACCACTACTTGGAACCAGCAATCCAATAATCTGGGTGCA
TCCAGTAAGTACGTAACATCTGTTTTGATGGATGCTGGTAACACCGGTGTTATCACTATTACGTATGTCGCTGACCAAGT
GGGCTTGCCGACTGCCGGTAACACTCTGATTCTGTCTCCCTATATCAATGATGGTAACACTCGCACTGCTCTGGCGACTG
CAGTGGCTGCTGGCACTCGTGGCACCATTGATTGGGCTTGCACTTCGGCATCGAACGCGACTGCCACTGCTCAGGGCTTC
ACCGGCATGGCAGCTGGTAGTGTGCCGCAAGAGTTTGCTCCTGCTCAGTGCCGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 3JYZ
  PDB 3JZZ

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

43.719

100

0.489
  pilA Ralstonia pseudosolanacearum GMI1000

40.556

100

0.41
  pilA2 Legionella pneumophila strain ERS1305867

40.341

98.876

0.399
  pilA2 Legionella pneumophila str. Paris

39.773

98.876

0.393