Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KI216_RS01305 Genome accession   NZ_AP024595
Coordinates   291658..293367 (-) Length   569 a.a.
NCBI ID   WP_005013293.1    Uniprot ID   -
Organism   Acinetobacter bouvetii strain JCM 18991     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 286658..298367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI216_RS01275 (ACBO_02420) - 286731..287054 (+) 324 WP_005013307.1 pyrimidine/purine nucleoside phosphorylase -
  KI216_RS01280 (ACBO_02430) rlmB 287193..287942 (+) 750 WP_005013305.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  KI216_RS01285 (ACBO_02440) - 287971..288891 (+) 921 WP_005013303.1 DMT family transporter -
  KI216_RS01290 (ACBO_02450) coaE 288888..289496 (-) 609 WP_005013302.1 dephospho-CoA kinase -
  KI216_RS16170 (ACBO_02460) pilD 289506..290369 (-) 864 WP_005013298.1 prepilin peptidase Machinery gene
  KI216_RS01300 (ACBO_02470) pilC 290393..291622 (-) 1230 WP_005013295.1 type II secretion system F family protein Machinery gene
  KI216_RS01305 (ACBO_02480) pilB 291658..293367 (-) 1710 WP_005013293.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KI216_RS01310 (ACBO_02490) tpiA 293643..294434 (+) 792 WP_005013292.1 triose-phosphate isomerase -
  KI216_RS01315 (ACBO_02500) secG 294448..294777 (+) 330 WP_005013290.1 preprotein translocase subunit SecG -
  KI216_RS01345 (ACBO_02510) rimP 295718..296254 (+) 537 WP_005013289.1 ribosome maturation factor RimP -
  KI216_RS01350 (ACBO_02520) nusA 296278..297762 (+) 1485 WP_005013286.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63461.41 Da        Isoelectric Point: 4.7235

>NTDB_id=86293 KI216_RS01305 WP_005013293.1 291658..293367(-) (pilB) [Acinetobacter bouvetii strain JCM 18991]
MTALQGSPRFTGFIRRLVEEGHVSAEKMREALLSAQKANQDIVAYLTKELNIPSLTVAETISAEFGEPIFDISAYDANLI
IREDIDEKLITRYRVLPIYKHASTLYVATSNPTNIEAVDAIRFSSKMNTETIIVEHHKLEKLIEQQFAEESTFDFDDDFD
LDIATDAPDQNTDDEEEEKGEEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRSIANPPLQLANRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFMQALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKEAVEIPQQSLLEMGFT
EDDLKNPDFQVYQPVGCPECREGYKGRVGIYEVMKVTPEISRIIMEDGNALLIAEAANQAGFNNLRRSGLVKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=86293 KI216_RS01305 WP_005013293.1 291658..293367(-) (pilB) [Acinetobacter bouvetii strain JCM 18991]
ATGACAGCACTACAGGGATCTCCAAGATTTACAGGGTTTATTCGGCGCCTGGTCGAAGAAGGACACGTATCTGCAGAAAA
AATGCGTGAAGCGCTCTTAAGCGCCCAAAAAGCCAATCAGGACATTGTCGCGTACCTGACCAAAGAACTGAACATTCCGT
CATTGACTGTGGCGGAAACCATTTCAGCAGAATTTGGCGAACCGATTTTTGACATTTCCGCTTATGATGCCAATCTGATT
ATCCGCGAAGATATTGACGAGAAGCTGATCACGCGCTACCGCGTGCTGCCGATTTACAAACATGCCAGCACACTGTATGT
GGCAACCAGCAATCCGACCAACATTGAAGCTGTGGATGCAATCCGCTTCAGCAGCAAAATGAACACGGAAACCATTATTG
TTGAGCACCATAAGCTGGAAAAGCTGATTGAGCAGCAATTCGCGGAAGAAAGCACTTTTGATTTTGATGATGATTTCGAT
CTTGATATTGCGACAGATGCCCCTGACCAGAACACCGATGATGAAGAAGAAGAAAAAGGCGAAGAAGCGCCTATTGTTAA
ATACATCAATAAGCTGCTGGTCGATGCCATCCGCATGGGCGCTTCGGATTTGCACTTTGAACCCTATGAAAAATCTTACC
GTGTCCGCTACCGCGTCGATGGCGTGCTGAGATCAATTGCCAACCCGCCCTTGCAGCTGGCGAATCGCTTAGCATCCCGC
CTCAAAGTAATGTCGCAAATGGACATTTCTGAAAAGCGCGTTCCGCAAGACGGCCGCATTAAGCTGAAATTATCCAAAAG
CAAGGCGATCGACTTCCGTGTAAACTCACTGCCTACGCTGTTCGGTGAAAAACTGGTGCTGCGTATTCTGGATCCATCCA
GCGCCATGCTCGGGATTGATGCGCTGGGTTATGAACCTGATCAAAAAGAACTGTTTATGCAGGCTTTAGAAAAACCCCAA
GGCATGCTGCTGATTACTGGTCCAACGGGTTCAGGTAAAACGGTATCTTTATATACTGGCCTGAATATTTTAAACCGGGA
AGACACCAATATATCCACCGCAGAAGACCCGGTAGAGATTAATTTGGAAGGAATCAATCAAGTCAATGTCAACAATAAAG
TGGGCTTAACCTTCTCCGCCGCATTAAAATCTTTCCTGCGCCAGGATCCGGATATTGTCATGGTTGGTGAGATTCGTGAT
TTAGAAACGGCTGAAATTGCCATTAAAGCGGCGCAGACAGGCCATATGGTAATGTCGACGCTGCATACCAACAGCGCGCC
TGAAACGTTGACGCGTCTGCGCAATATGGGTGTGCCTTCATTCAATATCGCAACTTCAGTCAATCTGGTGATTGCACAGC
GCTTAGCGCGCCGCCTGTGCTCGCAATGTAAAGAAGCAGTGGAAATTCCACAGCAAAGCCTGCTGGAAATGGGCTTTACA
GAAGATGATCTGAAAAACCCAGACTTTCAAGTTTACCAGCCTGTCGGCTGTCCAGAATGCCGTGAAGGCTATAAAGGCCG
TGTCGGTATTTATGAAGTAATGAAAGTGACGCCGGAGATTTCACGCATTATTATGGAAGACGGCAACGCGCTTCTTATTG
CAGAGGCTGCAAATCAAGCAGGATTTAACAATTTGCGCCGTTCAGGCTTAGTTAAGGTTATGCAGGGGGTGACTTCTCTT
CAGGAAGTCAATCGCGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.526

100

0.807
  pilB Acinetobacter baylyi ADP1

75.439

100

0.756
  pilB Legionella pneumophila strain ERS1305867

51.675

99.649

0.515
  pilF Neisseria gonorrhoeae MS11

49.554

98.594

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

49.283

98.067

0.483
  pilB Vibrio cholerae strain A1552

51.248

91.564

0.469
  pilB Vibrio campbellii strain DS40M4

47.935

97.891

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

34.258

100

0.369
  pilF Thermus thermophilus HB27

36.842

100

0.369

Multiple sequence alignment