Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KI216_RS16170 Genome accession   NZ_AP024595
Coordinates   289506..290369 (-) Length   287 a.a.
NCBI ID   WP_005013298.1    Uniprot ID   N9DLC6
Organism   Acinetobacter bouvetii strain JCM 18991     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 284506..295369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI216_RS01275 (ACBO_02420) - 286731..287054 (+) 324 WP_005013307.1 pyrimidine/purine nucleoside phosphorylase -
  KI216_RS01280 (ACBO_02430) rlmB 287193..287942 (+) 750 WP_005013305.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  KI216_RS01285 (ACBO_02440) - 287971..288891 (+) 921 WP_005013303.1 DMT family transporter -
  KI216_RS01290 (ACBO_02450) coaE 288888..289496 (-) 609 WP_005013302.1 dephospho-CoA kinase -
  KI216_RS16170 (ACBO_02460) pilD 289506..290369 (-) 864 WP_005013298.1 prepilin peptidase Machinery gene
  KI216_RS01300 (ACBO_02470) pilC 290393..291622 (-) 1230 WP_005013295.1 type II secretion system F family protein Machinery gene
  KI216_RS01305 (ACBO_02480) pilB 291658..293367 (-) 1710 WP_005013293.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KI216_RS01310 (ACBO_02490) tpiA 293643..294434 (+) 792 WP_005013292.1 triose-phosphate isomerase -
  KI216_RS01315 (ACBO_02500) secG 294448..294777 (+) 330 WP_005013290.1 preprotein translocase subunit SecG -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 32181.58 Da        Isoelectric Point: 8.0605

>NTDB_id=86322 KI216_RS16170 WP_005013298.1 289506..290369(-) (pilD) [Acinetobacter bouvetii strain JCM 18991]
MFSNIISYFIENPTALYIAVGLFSLCIGSFLNVVIYRTPKMMEQEWRTDCQFLLHPEQPIIDESKITLSKPASTCPKCHS
PIRWYQNIPVISWLILRGKCGACQNPISIRYPLIELLTAGCSLIVVTVFGPTLQMLFGLLFTWVLIALTFIDFDTQLLPD
RYTLPLAALGLGINSFAIYTSPVSSIWGYIIGFLCLWIVYYLFKIVTGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSS
LVGAIIGIILLKIRKENQPFAFGPYIAIAGWIAFLWGEQIMKIYLGQ

Nucleotide


Download         Length: 864 bp        

>NTDB_id=86322 KI216_RS16170 WP_005013298.1 289506..290369(-) (pilD) [Acinetobacter bouvetii strain JCM 18991]
TTGTTCTCCAATATTATTTCCTATTTTATCGAAAATCCAACAGCGCTTTATATTGCTGTTGGCCTATTCAGCCTGTGCAT
AGGCAGTTTTTTGAATGTGGTTATTTACCGCACGCCAAAAATGATGGAACAGGAGTGGCGCACTGATTGCCAGTTTCTGC
TGCATCCTGAACAGCCGATTATTGATGAAAGCAAAATCACGCTGAGCAAACCCGCTTCAACCTGTCCAAAGTGCCACAGT
CCGATTCGGTGGTATCAAAATATTCCTGTGATCAGCTGGCTGATATTGCGCGGTAAATGCGGCGCCTGCCAAAACCCGAT
CAGCATCCGTTATCCGCTGATTGAACTGCTGACCGCAGGATGTTCATTGATTGTCGTCACCGTCTTTGGCCCAACGCTGC
AAATGCTGTTCGGCTTGCTGTTCACGTGGGTCTTAATCGCCCTGACATTTATTGATTTTGATACTCAGCTGCTTCCTGAC
CGCTATACGCTCCCTTTGGCGGCTTTAGGCTTAGGCATCAATAGCTTCGCTATCTATACTTCTCCGGTATCATCGATTTG
GGGGTATATCATTGGCTTCCTTTGCCTTTGGATTGTCTATTACCTCTTTAAAATTGTGACCGGCAAAGAAGGCATGGGCT
ACGGAGATTTTAAACTTCTGGCAGCGCTAGGCGCATGGATGGGGCCGCTGATGCTGCCGCTGATTGTGCTCTTATCATCC
TTAGTCGGCGCAATTATCGGCATTATCCTACTGAAAATCCGCAAGGAAAATCAGCCTTTTGCTTTTGGCCCCTATATTGC
CATTGCCGGCTGGATCGCCTTTCTCTGGGGTGAGCAGATTATGAAAATTTATTTGGGTCAATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(140-248)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9DLC6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

83.803

98.955

0.829
  pilD Acinetobacter baumannii D1279779

83.451

98.955

0.826
  pilD Vibrio cholerae strain A1552

56.506

93.728

0.53
  pilD Vibrio campbellii strain DS40M4

55.597

93.38

0.519
  pilD Neisseria gonorrhoeae MS11

46.52

95.122

0.443

Multiple sequence alignment