Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QWJ31_RS12085 Genome accession   NZ_CP130340
Coordinates   632717..634039 (-) Length   440 a.a.
NCBI ID   WP_390885701.1    Uniprot ID   -
Organism   Cupriavidus gilardii strain M5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 627717..639039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QWJ31_RS12055 (QWJ31_12055) - 628335..628739 (+) 405 WP_053822561.1 NUDIX domain-containing protein -
  QWJ31_RS12060 (QWJ31_12060) - 628767..628955 (-) 189 WP_053822560.1 DNA gyrase inhibitor YacG -
  QWJ31_RS12065 (QWJ31_12065) zapD 628962..629720 (-) 759 WP_006578256.1 cell division protein ZapD -
  QWJ31_RS12070 (QWJ31_12070) coaE 629971..630603 (-) 633 WP_053822559.1 dephospho-CoA kinase -
  QWJ31_RS12075 (QWJ31_12075) - 630616..631557 (-) 942 WP_269465299.1 A24 family peptidase -
  QWJ31_RS12080 (QWJ31_12080) - 631559..632683 (-) 1125 WP_082371553.1 type II secretion system F family protein -
  QWJ31_RS12085 (QWJ31_12085) pilF 632717..634039 (-) 1323 WP_390885701.1 GspE/PulE family protein Machinery gene
  QWJ31_RS12095 (QWJ31_12095) ispB 634238..635167 (-) 930 WP_053822557.1 octaprenyl diphosphate synthase -
  QWJ31_RS12100 (QWJ31_12100) rplU 635711..636022 (+) 312 WP_006576525.1 50S ribosomal protein L21 -
  QWJ31_RS12105 (QWJ31_12105) rpmA 636086..636346 (+) 261 WP_053822556.1 50S ribosomal protein L27 -
  QWJ31_RS12110 (QWJ31_12110) obgE 636484..637581 (+) 1098 WP_053822555.1 GTPase ObgE -
  QWJ31_RS12115 (QWJ31_12115) proB 637644..638768 (+) 1125 WP_053822554.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 47384.88 Da        Isoelectric Point: 8.3207

>NTDB_id=858026 QWJ31_RS12085 WP_390885701.1 632717..634039(-) (pilF) [Cupriavidus gilardii strain M5]
MPVPAGASHTGPLPHCVPPAMIAMTAPSDAPIEYDPAMPSVDDAPVVRFVQTLLAEACRRGASDLHFEPYEGFYRVRFRI
DGQLHEVARPPLDIRDRIATRIKVLARLDIAEKRVPQDGRMRFVVPRRDGEATPDGAAGRAVDLRVSTLPTLFGEKIVLR
MLAASSVRLDMDSLGLEPTQLAVLLERLRRPHGMVLVTGPTGSGKTVSLYALLNLLNQHERNISTAEDPAEIQLAGINQV
NIHDKIGLNFPTVLRALLRQDPDVIMVGEIRDAETADVAVKAAQTGHLVLSTLHTNDAPGTLTRLAQLGVAPFHLAASVL
VITAQRLARMLCACKRPVPVPLSALREAGFADTLLDGDWQPCQPVGCAACRGSGYRGRCGIHQVMPVSDAMQNIILSRGS
ARALAQQARREGVLSLREAGLLKVRAGTTSLAEVLATTNP

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=858026 QWJ31_RS12085 WP_390885701.1 632717..634039(-) (pilF) [Cupriavidus gilardii strain M5]
ATTCCCGTTCCCGCCGGCGCATCGCACACTGGGCCACTCCCGCACTGCGTCCCGCCCGCCATGATCGCCATGACCGCCCC
GTCCGATGCGCCGATCGAATACGACCCCGCCATGCCATCGGTCGACGACGCGCCGGTCGTGCGCTTCGTCCAGACGCTGC
TGGCCGAGGCCTGCCGGCGCGGCGCCTCGGACCTGCACTTCGAGCCGTACGAAGGCTTCTATCGCGTGCGCTTTCGCATC
GATGGGCAGCTGCACGAGGTCGCCCGTCCGCCGCTGGACATTCGCGATCGCATCGCGACGCGGATCAAGGTGCTGGCGCG
GCTGGACATCGCCGAGAAGCGTGTACCGCAGGATGGCCGCATGCGGTTCGTGGTGCCGCGGCGCGACGGCGAGGCGACGC
CTGACGGGGCCGCCGGCCGCGCGGTCGATCTGCGCGTATCGACGCTGCCGACGCTGTTCGGCGAAAAGATCGTGCTGCGC
ATGCTGGCCGCGTCCAGTGTGCGGCTCGATATGGACAGCCTCGGCCTCGAGCCGACGCAGCTGGCCGTGCTGCTCGAGCG
GCTGCGCCGTCCGCACGGCATGGTGCTGGTCACCGGTCCGACCGGCAGCGGCAAGACCGTTTCGCTGTACGCCCTCCTGA
ATCTGCTGAACCAGCACGAGCGCAATATCTCGACGGCCGAGGACCCGGCCGAGATCCAGCTGGCCGGCATCAACCAGGTC
AATATCCACGACAAGATTGGCCTGAACTTCCCCACCGTGCTGCGAGCGTTGCTGCGGCAGGACCCGGACGTGATCATGGT
CGGCGAGATCCGCGATGCCGAAACCGCCGATGTCGCGGTCAAGGCGGCGCAGACCGGCCATCTGGTACTGTCGACGCTGC
ACACCAACGATGCGCCGGGCACGCTGACACGGCTCGCGCAGCTTGGCGTCGCGCCCTTTCACCTGGCCGCCAGCGTGCTG
GTGATCACCGCGCAGCGGCTGGCCCGCATGCTGTGCGCCTGCAAACGTCCGGTCCCGGTGCCGCTGTCCGCGCTGCGCGA
GGCCGGTTTCGCGGACACCTTGCTCGACGGCGACTGGCAGCCCTGCCAGCCGGTCGGCTGCGCCGCGTGCCGCGGCAGCG
GTTATCGCGGGCGCTGCGGCATCCATCAGGTGATGCCGGTCTCCGACGCGATGCAGAACATCATCCTGTCGCGGGGCAGT
GCCCGTGCGCTGGCCCAGCAGGCGCGCCGCGAGGGCGTGCTATCGTTGCGCGAGGCGGGGCTGCTCAAGGTCAGGGCGGG
CACGACCTCGCTGGCCGAGGTGCTGGCGACGACCAATCCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(53-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

57

90.909

0.518
  pilB Acinetobacter baumannii D1279779

53.465

91.818

0.491
  pilB Acinetobacter baylyi ADP1

54.198

89.318

0.484
  pilB Vibrio cholerae strain A1552

52.736

91.364

0.482
  pilB Legionella pneumophila strain ERS1305867

49.635

93.409

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

51.01

90

0.459
  pilB Vibrio campbellii strain DS40M4

50.758

90

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

42.584

95

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.537

91.364

0.389
  pilF Thermus thermophilus HB27

41.584

91.818

0.382
  pilB Glaesserella parasuis strain SC1401

38.249

98.636

0.377
  pilB Haemophilus influenzae 86-028NP

38.018

98.636

0.375
  pilB Haemophilus influenzae Rd KW20

37.788

98.636

0.373