Detailed information NTG0085793

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: I0P84_RS01755
• Genome accession: NZ_AP024524
• Coordinates: 382839..383897 (+)
• Length: 352 a.a.
• NCBI ID: WP_004787132.1
• Uniprot ID: N8VCV1
• Organism: Acinetobacter variabilis strain RYU24
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 377839..388897

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I0P84_RS01740 (RYU24_03350)	mraY	378105..379223 (+)	1119	WP_175966246.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
I0P84_RS01745 (RYU24_03360)	-	379282..380085 (+)	804	WP_175966244.1	putative RNA methyltransferase	-
I0P84_RS01750 (RYU24_03370)	ponA	380144..382672 (-)	2529	WP_175966243.1	penicillin-binding protein PBP1a	-
I0P84_RS01755 (RYU24_03380)	pilM	382839..383897 (+)	1059	WP_004787132.1	pilus assembly protein PilM	Machinery gene
I0P84_RS01760 (RYU24_03390)	comN	383897..384535 (+)	639	WP_004787129.1	PilN domain-containing protein	Machinery gene
I0P84_RS01765 (RYU24_03400)	comO	384532..385248 (+)	717	WP_175966241.1	type 4a pilus biogenesis protein PilO	Machinery gene
I0P84_RS01770 (RYU24_03410)	pilP	385248..385775 (+)	528	WP_034169727.1	pilus assembly protein PilP	Machinery gene
I0P84_RS01775 (RYU24_03420)	pilQ	385837..387957 (+)	2121	WP_214002286.1	type IV pilus secretin PilQ family protein	Machinery gene
I0P84_RS01780 (RYU24_03430)	aroK	387994..388530 (+)	537	WP_214001837.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39059.59 Da
• Isoelectric Point: 4.5754

>NTDB_id=85793 I0P84_RS01755 WP_004787132.1 382839..383897(+) (pilM) [Acinetobacter variabilis strain RYU24]
MRRLYRKPNKGLIGVDISSTSVKVLELSVKNGRYWVESYALVPLPESSVVEKNILNPEAVGDALGRAINLANTQSSQSAF
AIPTSMVITKIIEMDADMSDDEREVQIREDAEQYIPFPLDEASLDFEVLPDRLTNPNRVNVLLVATRIENVEARSEVLQI
ANLNPKIADVESFALENAFKVFSDTLPMGVNTVGILDIGHSMTTLSVMQNNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKTRALPDDYDIEVLEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=85793 I0P84_RS01755 WP_004787132.1 382839..383897(+) (pilM) [Acinetobacter variabilis strain RYU24]
GTGCGCAGGTTATATCGTAAGCCAAATAAGGGGTTAATAGGAGTCGATATTAGTTCGACTTCTGTTAAAGTTTTAGAGCT
CTCTGTAAAGAACGGTCGGTACTGGGTAGAAAGTTATGCTTTGGTCCCACTACCAGAAAGTAGTGTCGTTGAAAAAAACA
TTTTAAATCCAGAAGCGGTGGGTGATGCGTTGGGGCGTGCGATTAATTTGGCCAATACCCAGTCCAGTCAGTCAGCTTTT
GCGATTCCAACTTCTATGGTCATCACCAAAATCATCGAAATGGATGCCGATATGTCGGATGATGAACGTGAAGTTCAGAT
TCGTGAAGATGCTGAACAATATATTCCTTTCCCACTTGACGAAGCCAGCCTGGACTTCGAAGTTCTGCCAGACCGTTTAA
CCAATCCGAACCGGGTCAATGTCCTGTTGGTAGCGACCCGAATAGAAAATGTTGAAGCGCGTTCTGAAGTACTGCAAATT
GCCAATCTTAATCCAAAAATTGCAGATGTCGAAAGTTTTGCATTAGAAAATGCATTCAAGGTGTTTTCGGATACCTTACC
GATGGGGGTAAATACTGTCGGGATCCTGGATATTGGTCATAGTATGACGACCTTATCCGTCATGCAGAATAACAAGATCA
TTTATACCCGTGAGCAGGTTTTTGGTGGCAAACAGCTGACTCAGGAAATCCAGAACCGTTATGGATTGTCTTTTGAGGAA
GCAGGTCGTGCTAAAAAAACCCGTGCCTTACCGGATGACTATGACATCGAAGTGCTGGAACCTTTTCTAGATGCAGTCGT
GCAGCAGGCAGCGCGTTCATTACAGTTCTTCTTCTCTTCATCTCAGTTTAACGAGATTGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGTCTGGCAAAACTATTACAGCAAAAACTCGGCTACCGGGTCACGATTGCCAATCCGTTCCTG
CAAATGGGCTTTTCTCCTCAAATTGATATTAAAAAAATTGAAAATGACGCGTCCTCACTCATGGTTGCATGTGGTTTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8VCV1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.511	100	0.855
comM	Acinetobacter nosocomialis M2	85.511	100	0.855
comM	Acinetobacter baylyi ADP1	78.977	100	0.79
pilM	Legionella pneumophila strain ERS1305867	39.831	100	0.401