Detailed information NTG0857875

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: Q3F87_RS02540
• Genome accession: NZ_CP130328
• Coordinates: 357364..358422 (+)
• Length: 352 a.a.
• NCBI ID: WP_004787132.1
• Uniprot ID: N8VCV1
• Organism: Acinetobacter variabilis strain TB2-2B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 352364..363422

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q3F87_RS02525 (Q3F87_02525)	mraY	352630..353748 (+)	1119	WP_004787138.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
Q3F87_RS02530 (Q3F87_02530)	-	353807..354610 (+)	804	WP_180104160.1	putative RNA methyltransferase	-
Q3F87_RS02535 (Q3F87_02535)	ponA	354669..357197 (-)	2529	WP_302700346.1	penicillin-binding protein PBP1a	-
Q3F87_RS02540 (Q3F87_02540)	pilM	357364..358422 (+)	1059	WP_004787132.1	pilus assembly protein PilM	Machinery gene
Q3F87_RS02545 (Q3F87_02545)	comN	358422..359060 (+)	639	WP_179992180.1	PilN domain-containing protein	Machinery gene
Q3F87_RS02550 (Q3F87_02550)	comO	359057..359773 (+)	717	WP_159122691.1	type 4a pilus biogenesis protein PilO	Machinery gene
Q3F87_RS02555 (Q3F87_02555)	pilP	359773..360300 (+)	528	WP_034169727.1	pilus assembly protein PilP	Machinery gene
Q3F87_RS02560 (Q3F87_02560)	pilQ	360317..362482 (+)	2166	WP_302700347.1	type IV pilus secretin PilQ family protein	Machinery gene
Q3F87_RS02565 (Q3F87_02565)	aroK	362519..363055 (+)	537	WP_005233593.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39059.59 Da
• Isoelectric Point: 4.5754

>NTDB_id=857875 Q3F87_RS02540 WP_004787132.1 357364..358422(+) (pilM) [Acinetobacter variabilis strain TB2-2B]
MRRLYRKPNKGLIGVDISSTSVKVLELSVKNGRYWVESYALVPLPESSVVEKNILNPEAVGDALGRAINLANTQSSQSAF
AIPTSMVITKIIEMDADMSDDEREVQIREDAEQYIPFPLDEASLDFEVLPDRLTNPNRVNVLLVATRIENVEARSEVLQI
ANLNPKIADVESFALENAFKVFSDTLPMGVNTVGILDIGHSMTTLSVMQNNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKTRALPDDYDIEVLEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=857875 Q3F87_RS02540 WP_004787132.1 357364..358422(+) (pilM) [Acinetobacter variabilis strain TB2-2B]
GTGCGCAGGTTATATCGTAAGCCAAATAAGGGGTTAATAGGAGTCGATATTAGTTCGACTTCTGTTAAAGTTTTAGAGCT
CTCTGTAAAGAACGGTCGGTACTGGGTAGAAAGTTATGCTTTAGTCCCATTACCAGAAAGTAGTGTCGTTGAAAAAAATA
TTTTAAATCCAGAAGCGGTGGGTGATGCGTTGGGGCGTGCGATTAATTTGGCCAATACCCAGTCCAGTCAGTCAGCCTTT
GCGATTCCAACTTCTATGGTCATCACCAAGATCATCGAAATGGATGCCGATATGTCGGATGATGAACGTGAAGTTCAGAT
TCGTGAAGATGCCGAACAATATATTCCTTTCCCACTTGATGAAGCCAGTCTGGACTTTGAAGTTCTGCCAGACCGTTTAA
CCAATCCGAACCGGGTTAATGTCCTGTTGGTAGCGACCCGAATCGAAAATGTTGAAGCGCGTTCTGAAGTACTGCAAATT
GCCAATCTCAATCCAAAAATTGCAGATGTCGAAAGTTTTGCATTAGAAAATGCATTCAAGGTGTTTTCGGATACCTTGCC
GATGGGGGTAAATACTGTCGGGATCCTGGATATTGGTCATAGTATGACGACCTTATCCGTCATGCAGAATAACAAGATCA
TTTATACTCGTGAGCAGGTTTTTGGTGGTAAGCAGCTGACTCAGGAAATCCAGAACCGTTATGGGTTATCTTTTGAGGAA
GCAGGGCGTGCTAAAAAAACCCGTGCCTTACCGGATGATTATGATATCGAAGTGCTGGAACCTTTCCTGGATGCAGTCGT
GCAGCAGGCAGCGCGTTCATTACAGTTCTTCTTCTCTTCATCTCAGTTTAACGAGATTGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGTCTGGCAAAACTATTACAGCAAAAACTCGGCTACCGGGTCACGATTGCCAATCCATTCCTG
CAAATGGGCTTTTCTCCTCAAATTGATATTAAAAAAATTGAAAATGACGCGTCCTCACTCATGGTTGCATGTGGTTTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8VCV1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.511	100	0.855
comM	Acinetobacter nosocomialis M2	85.511	100	0.855
comM	Acinetobacter baylyi ADP1	78.977	100	0.79
pilM	Legionella pneumophila strain ERS1305867	39.831	100	0.401