Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q3F87_RS02340 Genome accession   NZ_CP130328
Coordinates   315929..317638 (-) Length   569 a.a.
NCBI ID   WP_302700322.1    Uniprot ID   -
Organism   Acinetobacter variabilis strain TB2-2B     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 310929..322638
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q3F87_RS02310 (Q3F87_02310) - 310985..311308 (+) 324 WP_004787221.1 pyrimidine/purine nucleoside phosphorylase -
  Q3F87_RS02315 (Q3F87_02315) rlmB 311451..312200 (+) 750 WP_005233526.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  Q3F87_RS02320 (Q3F87_02320) - 312246..313163 (+) 918 WP_180033702.1 DMT family transporter -
  Q3F87_RS02325 (Q3F87_02325) coaE 313202..313807 (-) 606 WP_302700318.1 dephospho-CoA kinase -
  Q3F87_RS02330 (Q3F87_02330) pilD 313809..314669 (-) 861 WP_302700320.1 A24 family peptidase Machinery gene
  Q3F87_RS02335 (Q3F87_02335) pilC 314669..315898 (-) 1230 WP_004787210.1 type II secretion system F family protein Machinery gene
  Q3F87_RS02340 (Q3F87_02340) pilB 315929..317638 (-) 1710 WP_302700322.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Q3F87_RS02345 (Q3F87_02345) tpiA 317917..318708 (+) 792 WP_302700324.1 triose-phosphate isomerase -
  Q3F87_RS02350 (Q3F87_02350) secG 318722..319051 (+) 330 WP_302700326.1 preprotein translocase subunit SecG -
  Q3F87_RS02380 (Q3F87_02380) rimP 320014..320538 (+) 525 WP_166136388.1 ribosome maturation factor RimP -
  Q3F87_RS02385 (Q3F87_02385) nusA 320574..322058 (+) 1485 WP_004787201.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63406.51 Da        Isoelectric Point: 4.8399

>NTDB_id=857874 Q3F87_RS02340 WP_302700322.1 315929..317638(-) (pilB) [Acinetobacter variabilis strain TB2-2B]
MTALQGSPRFTGFIRRLVDEGVLSAEDMRTALANAKQEKTDIVAYLVSHHNLSPTTIAETISVEFGEPLFDIGAYDPGQI
LREDIDEKLITKHRILPIYKNANVLYVATSNPTNIEATDAIRFATKLNIETVIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDVDVDTADPTKEEDEKDKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQLANRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPKQSLLEMGFT
EADLQSPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLVKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=857874 Q3F87_RS02340 WP_302700322.1 315929..317638(-) (pilB) [Acinetobacter variabilis strain TB2-2B]
ATGACAGCATTGCAGGGGTCACCAAGATTTACGGGGTTTATTCGTCGCCTGGTCGATGAAGGGGTATTATCAGCTGAAGA
TATGCGGACTGCTTTAGCCAATGCCAAACAGGAAAAAACAGATATCGTGGCCTATTTGGTCAGTCATCATAATCTTTCCC
CTACTACCATTGCTGAAACGATCTCAGTAGAATTTGGTGAGCCTCTATTTGATATTGGTGCTTATGATCCGGGACAAATC
CTGCGTGAAGATATTGATGAAAAGCTGATTACCAAGCATCGTATTCTGCCAATTTATAAAAATGCCAATGTACTTTATGT
CGCCACGAGCAATCCGACTAATATTGAAGCAACGGATGCCATCCGCTTTGCGACTAAACTGAATATTGAAACCGTTATTG
TTGAACACAATAAACTCGAGAAACTGATCGAGCAAAACTTTACTGAAGAAAGTACTTTTGATTTTGATGATGATTTCGAT
CTGGATGTTGATGTCGACACAGCCGATCCAACGAAAGAAGAAGATGAAAAAGACAAGGGTGAAGAAGCCCCGATTGTTAA
ATATATTAATAAATTACTGATTGATGCCATTCGTATGGGGGCATCAGATTTACATTTTGAACCTTATGAAAAAATGTATC
GGGTACGCTATCGTGTCGATGGCGTTTTACGTCAGATTGCAACACCGCCGCTACAACTAGCAAACCGTTTGGCGTCACGT
TTAAAAGTTATGTCGCAGATGGACATTTCTGAGAAACGTGTTCCTCAAGACGGTCGTATTAAATTAAAGCTCTCAAAAAA
CAAAGCTATCGACTTCCGTGTTAACTCACTCCCTACCCTGTTTGGCGAAAAACTGGTTCTACGTATTCTAGATCCGTCTA
GTGCCATGCTAGGAATTGATGCTTTAGGTTATGAACCTGAGCAGAAAGAGTTATTCATGGAAGCCCTAGATAAACCGCAA
GGGATGTTACTGATTACCGGTCCTACCGGTTCCGGTAAAACCGTATCACTTTATACGGGCTTAAACATCTTGAACCGTGA
AGATACCAATATTTCTACTGCGGAAGATCCAGTCGAGATTAACCTGCAAGGGATTAATCAGGTTAACGTGAATAATAAAG
TTGGCCTGACTTTCGCAGCGGCATTAAAATCCTTCTTGCGTCAGGATCCTGATATTGTCATGGTCGGTGAGATCCGTGAT
CTGGAAACAGCTGAAATTGCAATCAAAGCTGCCCAAACAGGTCATATGGTGATGTCAACCCTGCACACCAACAGTGCTCC
GGAAACTCTGACCCGTTTACGCAATATGGGCGTCCCTTCTTTCAATATCGCGACCTCGGTTAATCTGGTGATTGCACAGC
GTCTGGCCCGTCGCTTGTGTTCACAATGCAAAAAGCCGGCTGATATTCCAAAGCAAAGCTTACTGGAGATGGGCTTTACT
GAAGCAGATTTACAAAGCCCGGAATTTCAGATTTACGAGCCAGTCGGCTGTAATGAATGTCGTGAAGGTTATAAGGGCCG
GGTCGGTATTTATGAAGTCATGAAGGTCACACCTGAGATTTCAAGAATTATTATGGAAGATGGCAATGCCATTGAAATTG
CTGAAGCTTCCGCACGTGCAGGATTTAACAATTTACGCCGATCAGGGTTAGTGAAGGTTATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTAACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.053

100

0.812
  pilB Acinetobacter baylyi ADP1

76.667

100

0.768
  pilB Legionella pneumophila strain ERS1305867

50.883

99.473

0.506
  pilF Neisseria gonorrhoeae MS11

49.02

98.594

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

49.013

97.891

0.48
  pilB Vibrio campbellii strain DS40M4

48.644

97.188

0.473
  pilB Vibrio cholerae strain A1552

50.87

90.861

0.462
  pilF Thermus thermophilus HB27

38.489

100

0.385
  pilB/pilB1 Synechocystis sp. PCC 6803

34.747

100

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.138

97.012

0.36