Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Q2F48_RS02235 Genome accession   NZ_CP130164
Coordinates   453494..455200 (-) Length   568 a.a.
NCBI ID   WP_041213997.1    Uniprot ID   A0A2X4NBG7
Organism   Aeromonas caviae strain WZDHY22015     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 448494..460200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q2F48_RS02205 (Q2F48_02205) mutT 449112..449516 (+) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -
  Q2F48_RS02210 (Q2F48_02210) yacG 449574..449768 (-) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  Q2F48_RS02215 (Q2F48_02215) zapD 449779..450501 (-) 723 WP_279961679.1 cell division protein ZapD -
  Q2F48_RS02220 (Q2F48_02220) coaE 450539..451153 (-) 615 WP_109111851.1 dephospho-CoA kinase -
  Q2F48_RS02225 (Q2F48_02225) pilD 451172..452047 (-) 876 WP_119197036.1 A24 family peptidase Machinery gene
  Q2F48_RS02230 (Q2F48_02230) pilC 452125..453366 (-) 1242 WP_010675822.1 type II secretion system F family protein Machinery gene
  Q2F48_RS02235 (Q2F48_02235) pilB 453494..455200 (-) 1707 WP_041213997.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  Q2F48_RS02240 (Q2F48_02240) - 455208..455582 (-) 375 WP_103261553.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  Q2F48_RS02245 (Q2F48_02245) nadC 455893..456756 (-) 864 WP_052814467.1 carboxylating nicotinate-nucleotide diphosphorylase -
  Q2F48_RS02250 (Q2F48_02250) - 456760..457230 (-) 471 WP_104452204.1 retropepsin-like aspartic protease -
  Q2F48_RS02255 (Q2F48_02255) ampD 457359..457931 (+) 573 WP_103244159.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  Q2F48_RS02260 (Q2F48_02260) pdhR 458208..458972 (+) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62566.95 Da        Isoelectric Point: 5.8214

>NTDB_id=857419 Q2F48_RS02235 WP_041213997.1 453494..455200(-) (pilB) [Aeromonas caviae strain WZDHY22015]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=857419 Q2F48_RS02235 WP_041213997.1 453494..455200(-) (pilB) [Aeromonas caviae strain WZDHY22015]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCAGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCAAAGCCCTCG
CCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTTGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGTTTTGGCCTGCACACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGT
CTCAAGGTGATGGCGCGGCTCGATATTGCCGAACGGCGCCTGCCCCAGGATGGCCGGATCAAGCTCAAGATCTCACGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGTTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAACTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTGACCGGCCCGACGGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCCTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATAAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAGATCGCTATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTCACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAAAAGGAAGGGATGCGCACCCTGCGCACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGTCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2X4NBG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.026

99.824

0.549
  pilB Acinetobacter baylyi ADP1

55.062

99.12

0.546
  pilB Legionella pneumophila strain ERS1305867

54.867

99.472

0.546
  pilB Vibrio cholerae strain A1552

53.415

100

0.537
  pilB Vibrio campbellii strain DS40M4

50.53

99.648

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.089

99.12

0.496
  pilF Neisseria gonorrhoeae MS11

46.561

99.824

0.465
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37