Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QZJ86_RS02200 Genome accession   NZ_CP129884
Coordinates   464726..466438 (+) Length   570 a.a.
NCBI ID   WP_301936066.1    Uniprot ID   -
Organism   Methylomonas montana strain MW1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459726..471438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QZJ86_RS02185 (QZJ86_02185) - 460303..460710 (-) 408 WP_301936059.1 hypothetical protein -
  QZJ86_RS02190 (QZJ86_02190) recD 460718..462352 (-) 1635 WP_301936061.1 exodeoxyribonuclease V subunit alpha -
  QZJ86_RS02195 (QZJ86_02195) thiC 462657..464525 (+) 1869 WP_301936063.1 phosphomethylpyrimidine synthase ThiC -
  QZJ86_RS02200 (QZJ86_02200) pilB 464726..466438 (+) 1713 WP_301936066.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QZJ86_RS02205 (QZJ86_02205) pilC 466441..467658 (+) 1218 WP_301936067.1 type II secretion system F family protein Machinery gene
  QZJ86_RS02210 (QZJ86_02210) pilD 467729..468595 (+) 867 WP_301936068.1 A24 family peptidase Machinery gene
  QZJ86_RS02215 (QZJ86_02215) coaE 468599..469210 (+) 612 WP_301936070.1 dephospho-CoA kinase -
  QZJ86_RS02220 (QZJ86_02220) zapD 469207..469980 (+) 774 WP_301936071.1 cell division protein ZapD -
  QZJ86_RS02225 (QZJ86_02225) - 469980..470183 (+) 204 WP_301936073.1 DNA gyrase inhibitor YacG -
  QZJ86_RS02230 (QZJ86_02230) - 470198..471142 (-) 945 WP_301936076.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63182.89 Da        Isoelectric Point: 6.0123

>NTDB_id=856189 QZJ86_RS02200 WP_301936066.1 464726..466438(+) (pilB) [Methylomonas montana strain MW1]
MATASSNIHFSGLVKCLIQQGLLTEDLAATCLQEAQKRKQPFISYLVENKYTDSKTVASLASAEFGVPFFDLDAIDISFL
PIKMVSEKLIRQHHALPLFKRGNRLFVATADPTNFQALDEIKFHTRHNTENILVEEDKLIKMIDKALEAADTSMKDLLDD
DLDNIDISAGDDRPAEEAMGSDVDDAPIVRFVNKILLDSIKRGVSDIHMEPFEKKFRIRFRADGILSEVASPPVGIANRI
ISRLKVMSRMDIAERRVPQDGRIKMQISKNRAIDFRVNTCPTLFGEKVVLRILDPTSAQIGIEKLGFEPDQQKSFLEAIH
KPYGMVLVTGPTGSGKTVSLYTGLNILNSIECNISTAEDPVEITVEGINQVNVNPKAGLTFAEALRAFLRQDPDIIMVGE
IRDLETASIAVKAAQTGHMVLSTLHTNDAPQTINRLVQMGIEPFNIVSAVVLIMAQRLARRLCEHCKQEVDYPESALLSA
GFKKEEIGTFKVYGPVGCEHCNNGYKGRVGIYQVMPITENMRALILNGGNSMQMAELSKTEGINDLRMSGLLKIKQGITS
LQEIDRVTKE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=856189 QZJ86_RS02200 WP_301936066.1 464726..466438(+) (pilB) [Methylomonas montana strain MW1]
ATGGCGACAGCATCTTCAAACATCCATTTCAGCGGCTTGGTCAAATGCCTGATTCAACAAGGCTTGCTGACTGAGGACTT
AGCGGCAACCTGTCTACAGGAGGCGCAGAAACGCAAGCAGCCGTTCATTAGCTATTTGGTCGAGAATAAGTACACGGACA
GTAAAACCGTGGCGTCGTTGGCGTCGGCGGAATTCGGTGTGCCGTTTTTCGATCTGGATGCGATCGATATTTCGTTTTTG
CCGATCAAAATGGTCAGCGAGAAATTGATCCGTCAACATCATGCCTTACCGTTGTTCAAGCGCGGTAACCGCTTATTCGT
GGCGACCGCCGATCCGACCAATTTTCAGGCTCTGGACGAGATCAAATTCCATACGCGTCACAATACCGAAAACATTTTGG
TCGAAGAAGACAAACTGATCAAAATGATAGACAAGGCGCTGGAAGCGGCCGATACGTCGATGAAGGATTTGTTGGACGAC
GATCTTGACAATATCGATATTTCCGCTGGCGATGATCGGCCTGCTGAAGAAGCGATGGGTTCCGATGTTGACGATGCGCC
TATTGTTCGTTTTGTGAATAAAATCCTGCTCGATTCGATCAAGCGCGGGGTGTCGGATATACACATGGAACCTTTCGAGA
AAAAATTCCGCATCCGTTTCCGGGCTGACGGCATCTTATCGGAAGTGGCCAGTCCGCCGGTCGGCATCGCTAACCGGATT
ATTTCCAGACTGAAAGTCATGTCGAGAATGGATATCGCCGAGCGGCGGGTGCCGCAGGACGGCCGGATCAAGATGCAGAT
TTCCAAGAATCGCGCGATCGACTTCCGGGTCAATACCTGTCCGACGCTGTTCGGCGAAAAAGTGGTTTTGAGGATTCTCG
ATCCAACCAGCGCCCAGATCGGTATCGAAAAACTCGGTTTCGAGCCCGATCAGCAGAAAAGTTTCTTGGAAGCGATTCAC
AAGCCTTACGGTATGGTGCTGGTCACCGGGCCGACCGGTAGCGGTAAAACCGTATCGCTCTATACCGGCTTGAATATTCT
AAATAGCATCGAATGCAATATTTCTACGGCTGAAGATCCGGTGGAGATCACCGTGGAAGGCATCAATCAGGTCAACGTCA
ACCCCAAGGCCGGCTTGACCTTTGCCGAAGCGCTACGCGCGTTCTTGCGGCAAGACCCCGACATCATCATGGTCGGCGAG
ATTCGCGATCTGGAAACCGCCAGTATCGCCGTCAAGGCGGCGCAAACCGGTCACATGGTATTGTCCACGCTGCATACCAA
CGATGCGCCGCAAACCATCAACCGGCTGGTGCAGATGGGTATCGAACCGTTCAATATCGTTTCGGCGGTGGTTTTGATCA
TGGCGCAACGGCTAGCCCGTCGCTTGTGCGAGCATTGCAAGCAAGAGGTCGATTATCCGGAAAGCGCCTTGCTGTCGGCC
GGATTTAAGAAGGAAGAAATCGGTACGTTCAAAGTATACGGTCCGGTGGGCTGCGAACATTGCAATAACGGTTACAAAGG
CCGGGTGGGGATTTACCAGGTGATGCCGATTACCGAAAACATGCGCGCGCTGATCCTGAATGGCGGCAATTCAATGCAAA
TGGCGGAATTGTCGAAAACTGAAGGCATCAACGATTTAAGAATGTCAGGGCTGTTAAAAATTAAGCAGGGCATTACCTCG
CTCCAAGAAATTGACCGGGTCACCAAGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

54.72

100

0.549
  pilB Acinetobacter baumannii D1279779

53.565

100

0.54
  pilB Acinetobacter baylyi ADP1

52.273

100

0.525
  pilF Neisseria gonorrhoeae MS11

49.013

97.719

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

45.907

98.596

0.453
  pilB Vibrio campbellii strain DS40M4

45.374

98.596

0.447
  pilB Vibrio cholerae strain A1552

49.507

88.947

0.44
  pilB/pilB1 Synechocystis sp. PCC 6803

38.636

100

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.137

99.825

0.381
  pilF Thermus thermophilus HB27

38.849

97.544

0.379