Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   QZH48_RS06255 Genome accession   NZ_CP129879
Coordinates   1307257..1308105 (+) Length   282 a.a.
NCBI ID   WP_301675526.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain CAB701     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1302257..1313105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QZH48_RS06230 (QZH48_06230) ilvN 1303014..1303490 (+) 477 WP_058212549.1 acetolactate synthase small subunit -
  QZH48_RS06235 (QZH48_06235) ilvC 1303536..1304558 (+) 1023 WP_301675523.1 ketol-acid reductoisomerase -
  QZH48_RS06240 (QZH48_06240) ilvA 1304614..1305864 (+) 1251 WP_301675524.1 threonine ammonia-lyase IlvA -
  QZH48_RS06245 (QZH48_06245) budA 1305980..1306690 (+) 711 WP_003131140.1 acetolactate decarboxylase -
  QZH48_RS06250 (QZH48_06250) - 1306731..1307111 (+) 381 WP_301675525.1 RidA family protein -
  QZH48_RS06255 (QZH48_06255) dprA 1307257..1308105 (+) 849 WP_301675526.1 DNA-processing protein DprA Machinery gene
  QZH48_RS06260 (QZH48_06260) topA 1308367..1310499 (+) 2133 WP_003131148.1 type I DNA topoisomerase -
  QZH48_RS06265 (QZH48_06265) trmFO 1310571..1311914 (+) 1344 WP_301675527.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  QZH48_RS06270 (QZH48_06270) xerS 1311959..1313029 (-) 1071 WP_195228624.1 tyrosine recombinase XerS -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 32091.12 Da        Isoelectric Point: 9.5086

>NTDB_id=856119 QZH48_RS06255 WP_301675526.1 1307257..1308105(+) (dprA) [Lactococcus lactis subsp. lactis strain CAB701]
MITNFDLYRWKKAGMSNLGVNKLLKFFHRYDKKISLRQMGQVAQLKSIPNFVEQYKNQDVSKLREEYKIYPSFSLLDEIY
PERLKEIYNPPVLLFYQGDIRLLKTPKLAFVGSRQASSQGIKAVQKIVTELNQNFTIVSGLAKGIDTASHLSAIKNKIPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLILSEYSVGEKPLRYHFPERNRIIAGLSHGVVVIEAKLRSGSLITCERALEEG
RDIFAVPGNIADGFSDGCNHLIQQGAKLIYQAQDILEEYLYD

Nucleotide


Download         Length: 849 bp        

>NTDB_id=856119 QZH48_RS06255 WP_301675526.1 1307257..1308105(+) (dprA) [Lactococcus lactis subsp. lactis strain CAB701]
ATGATAACAAATTTTGATTTATATCGTTGGAAAAAAGCTGGCATGTCCAATTTAGGAGTAAATAAACTTTTAAAATTTTT
TCATAGATATGATAAAAAAATAAGTTTAAGACAAATGGGACAAGTAGCACAACTTAAATCAATTCCCAACTTTGTTGAAC
AATATAAAAATCAAGATGTAAGTAAACTGAGAGAAGAGTATAAAATATACCCATCATTTTCTCTTCTTGATGAAATTTAT
CCCGAAAGGCTCAAGGAAATTTACAATCCACCGGTTCTATTATTTTATCAAGGAGATATAAGACTCTTAAAAACTCCTAA
ATTAGCTTTTGTTGGTAGTCGACAAGCAAGCTCACAAGGAATTAAAGCCGTTCAAAAAATTGTGACAGAGTTGAACCAAA
ATTTTACGATTGTCAGCGGACTCGCTAAAGGAATAGATACAGCAAGTCATCTTTCTGCCATTAAAAATAAAATCCCTACA
ATTGCAGTTATCGGTACAGGCTTAGATATCTTTTATCCTTTAGAAAATCGAAAAATTCAAGAATATCTTGCTAAAAACCA
ACTCATACTATCAGAATATTCAGTCGGAGAAAAACCACTTCGCTATCATTTTCCTGAACGTAATCGTATCATTGCGGGGC
TTTCACATGGTGTAGTAGTGATAGAAGCAAAGTTACGAAGTGGTAGTTTAATTACTTGTGAGCGCGCTCTGGAAGAAGGA
AGAGATATTTTCGCTGTACCAGGAAACATAGCAGATGGATTTTCAGATGGCTGTAATCATCTAATACAACAAGGAGCAAA
ATTAATCTATCAAGCCCAAGATATCTTAGAAGAATATTTGTATGATTAA

Domains

Predicted by InterproScan.

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(2-63)

DNA recombination-mediator protein A (DNA_processg_A)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Lactococcus lactis subsp. cremoris KW2

86.17

100

0.862
  dprA Streptococcus mutans UA159

59.712

98.582

0.589
  dprA/cilB/dalA Streptococcus mitis SK321

54.286

99.291

0.539
  dprA/cilB/dalA Streptococcus pneumoniae R6

53.571

99.291

0.532
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

53.571

99.291

0.532
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

53.571

99.291

0.532
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

53.571

99.291

0.532
  dprA/cilB/dalA Streptococcus pneumoniae D39

53.571

99.291

0.532
  dprA Bacillus subtilis subsp. subtilis str. 168

40.364

97.518

0.394
  dprA Legionella pneumophila strain ERS1305867

39.13

97.872

0.383
  dprA Latilactobacillus sakei subsp. sakei 23K

39.259

95.745

0.376
  dprA Haemophilus influenzae Rd KW20

38.889

95.745

0.372