Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V6S45_RS01945 Genome accession   NZ_CP145630
Coordinates   414046..415431 (-) Length   461 a.a.
NCBI ID   WP_001025166.1    Uniprot ID   -
Organism   Escherichia coli strain S1-SRL-01-C     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 409046..420431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6S45_RS01910 (V6S45_01910) mutT 409381..409779 (+) 399 WP_000736017.1 8-oxo-dGTP diphosphatase MutT -
  V6S45_RS01915 (V6S45_01915) yacG 409981..410178 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  V6S45_RS01920 (V6S45_01920) zapD 410188..410931 (-) 744 WP_001194721.1 cell division protein ZapD -
  V6S45_RS01925 (V6S45_01925) coaE 410931..411551 (-) 621 WP_001269517.1 dephospho-CoA kinase -
  V6S45_RS01930 (V6S45_01930) - 411576..411620 (+) 45 WP_120795372.1 protein YacM -
  V6S45_RS01935 (V6S45_01935) guaC 411776..412819 (+) 1044 WP_001217337.1 GMP reductase -
  V6S45_RS01940 (V6S45_01940) hofC 412854..414056 (-) 1203 WP_000157245.1 protein transport protein HofC -
  V6S45_RS01945 (V6S45_01945) pilB 414046..415431 (-) 1386 WP_001025166.1 type II secretion system protein GspE Machinery gene
  V6S45_RS01950 (V6S45_01950) pilA 415441..415881 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  V6S45_RS01955 (V6S45_01955) nadC 416084..416977 (-) 894 WP_001135190.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V6S45_RS01960 (V6S45_01960) ampD 417065..417616 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V6S45_RS01965 (V6S45_01965) ampE 417613..418467 (+) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  V6S45_RS01970 (V6S45_01970) aroP 418510..419883 (-) 1374 WP_072146659.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50630.28 Da        Isoelectric Point: 6.5796

>NTDB_id=851237 V6S45_RS01945 WP_001025166.1 414046..415431(-) (pilB) [Escherichia coli strain S1-SRL-01-C]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=851237 V6S45_RS01945 WP_001025166.1 414046..415431(-) (pilB) [Escherichia coli strain S1-SRL-01-C]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTGGTCGATGCCCCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGCAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACAGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGTCTTACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTCTCATCGGCGCTTACACTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGACAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.469

100

0.416
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Haemophilus influenzae 86-028NP

40.127

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Acinetobacter baylyi ADP1

45.478

83.948

0.382
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Acinetobacter baumannii D1279779

44.186

83.948

0.371
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36