Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V6S45_RS01950 Genome accession   NZ_CP145630
Coordinates   415441..415881 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain S1-SRL-01-C     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 410441..420881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6S45_RS01925 (V6S45_01925) coaE 410931..411551 (-) 621 WP_001269517.1 dephospho-CoA kinase -
  V6S45_RS01930 (V6S45_01930) - 411576..411620 (+) 45 WP_120795372.1 protein YacM -
  V6S45_RS01935 (V6S45_01935) guaC 411776..412819 (+) 1044 WP_001217337.1 GMP reductase -
  V6S45_RS01940 (V6S45_01940) hofC 412854..414056 (-) 1203 WP_000157245.1 protein transport protein HofC -
  V6S45_RS01945 (V6S45_01945) pilB 414046..415431 (-) 1386 WP_001025166.1 type II secretion system protein GspE Machinery gene
  V6S45_RS01950 (V6S45_01950) pilA 415441..415881 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  V6S45_RS01955 (V6S45_01955) nadC 416084..416977 (-) 894 WP_001135190.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V6S45_RS01960 (V6S45_01960) ampD 417065..417616 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V6S45_RS01965 (V6S45_01965) ampE 417613..418467 (+) 855 WP_000172019.1 beta-lactamase regulator AmpE -
  V6S45_RS01970 (V6S45_01970) aroP 418510..419883 (-) 1374 WP_072146659.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=851238 V6S45_RS01950 WP_000360904.1 415441..415881(-) (pilA) [Escherichia coli strain S1-SRL-01-C]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=851238 V6S45_RS01950 WP_000360904.1 415441..415881(-) (pilA) [Escherichia coli strain S1-SRL-01-C]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGTTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363