NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	NSY31_RS12295	Genome accession	NZ_CP127834
Coordinates	2535049..2535663 (-)	Length	204 a.a.
NCBI ID	WP_015240245.1	Uniprot ID	-
Organism	Bacillus velezensis strain Ag109
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2530049..2540663

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NSY31_RS12265 (NSY31_12265)	rpsT	2530352..2530618 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
NSY31_RS12270 (NSY31_12270)	holA	2530635..2531676 (-)	1042	Protein_2378	DNA polymerase III subunit delta	-
NSY31_RS12275 (NSY31_12275)	-	2531716..2531867 (-)	152	Protein_2379	hypothetical protein	-
NSY31_RS12280 (NSY31_12280)	-	2531908..2532042 (+)	135	WP_003152870.1	YqzM family protein	-
NSY31_RS12285 (NSY31_12285)	comEC	2532061..2534412 (-)	2352	WP_098081592.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NSY31_RS12290 (NSY31_12290)	-	2534413..2534982 (-)	570	WP_003152868.1	ComE operon protein 2	-
NSY31_RS12295 (NSY31_12295)	comEA	2535049..2535663 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
NSY31_RS12300 (NSY31_12300)	comER	2535722..2536543 (+)	822	WP_015240246.1	late competence protein ComER	-
NSY31_RS12305 (NSY31_12305)	-	2536612..2537349 (-)	738	WP_015240247.1	class I SAM-dependent methyltransferase	-
NSY31_RS12310 (NSY31_12310)	rsfS	2537346..2537702 (-)	357	WP_007408260.1	ribosome silencing factor	-
NSY31_RS12315 (NSY31_12315)	yqeK	2537726..2538280 (-)	555	WP_061860724.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
NSY31_RS12320 (NSY31_12320)	-	2538270..2538839 (-)	570	WP_098081593.1	nicotinate-nucleotide adenylyltransferase	-
NSY31_RS12325 (NSY31_12325)	yhbY	2538850..2539140 (-)	291	WP_098081594.1	ribosome assembly RNA-binding protein YhbY	-
NSY31_RS12330 (NSY31_12330)	aroE	2539134..2539976 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22209.32 Da Isoelectric Point: 5.5211

                                >NTDB_id=844540 NSY31_RS12295 WP_015240245.1 2535049..2535663(-) (comEA) [Bacillus velezensis strain Ag109]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=844540 NSY31_RS12295 WP_015240245.1 2535049..2535663(-) (comEA) [Bacillus velezensis strain Ag109]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTAAAAGGGGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0844540