Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QP552_RS04955 Genome accession   NZ_CP127408
Coordinates   1097149..1098243 (+) Length   364 a.a.
NCBI ID   WP_043033789.1    Uniprot ID   A0A0X3QW38
Organism   Stenotrophomonas sp. 59     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1092149..1103243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP552_RS04925 soxR 1092220..1092654 (+) 435 WP_106549861.1 redox-sensitive transcriptional activator SoxR -
  QP552_RS04930 - 1092739..1093341 (-) 603 WP_106549862.1 short chain dehydrogenase -
  QP552_RS04935 - 1093438..1094313 (+) 876 WP_286075175.1 LysR family transcriptional regulator -
  QP552_RS04940 proC 1094362..1095183 (-) 822 WP_111111566.1 pyrroline-5-carboxylate reductase -
  QP552_RS04945 - 1095202..1095879 (-) 678 WP_111111567.1 YggS family pyridoxal phosphate-dependent enzyme -
  QP552_RS04950 pilT 1095967..1097004 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  QP552_RS04955 pilU 1097149..1098243 (+) 1095 WP_043033789.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QP552_RS04960 - 1098633..1099616 (+) 984 WP_111111568.1 aldo/keto reductase -
  QP552_RS04965 - 1099688..1100587 (-) 900 WP_111111569.1 LysR family transcriptional regulator -
  QP552_RS04970 - 1100890..1101102 (+) 213 WP_286075178.1 DUF4287 domain-containing protein -
  QP552_RS04975 - 1101214..1101921 (+) 708 WP_099553961.1 YitT family protein -
  QP552_RS04980 - 1101998..1102543 (-) 546 WP_286075180.1 DNA-3-methyladenine glycosylase I -
  QP552_RS04985 - 1102632..1103198 (+) 567 WP_005408377.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40569.61 Da        Isoelectric Point: 6.7680

>NTDB_id=844143 QP552_RS04955 WP_043033789.1 1097149..1098243(+) (pilU) [Stenotrophomonas sp. 59]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=844143 QP552_RS04955 WP_043033789.1 1097149..1098243(+) (pilU) [Stenotrophomonas sp. 59]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACCGCCGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
AATCACGCAGACCCCGCTGACGCCCCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAAT
TCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGTGTCAGCTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAACTGAGCCTGCCGCCGATCATCAA
GACGCTGGCAATGACCAAGCGCGGCATCATCCTGTTTGTCGGCGCCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGA
TGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGATCCGATCGAGTTCGTGCACAAGCACGAA
GGCTGCATCATCACCCAGCGTGAGGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATTGGTGAGGTGCGTACGCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAGACCGGTCACC
TGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGATCGCCGC
AACCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGTTC
GCGCAAGGTCGCCATGGAGATCCTGCTCGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAACTGTACCAGGCCGGCGAG
ATCAGCTATGAAGACGCGCTGCGCTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
CGCACGCACCCTGTCGCAGGGGTTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X3QW38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.715

98.352

0.676
  pilU Acinetobacter baylyi ADP1

64.08

95.604

0.613
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.615

89.286

0.363