Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QP512_RS15950 Genome accession   NZ_CP127407
Coordinates   3460744..3462474 (-) Length   576 a.a.
NCBI ID   WP_286069618.1    Uniprot ID   -
Organism   Stenotrophomonas sp. 57     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3455744..3467474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP512_RS15935 sucC 3455993..3457162 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  QP512_RS15940 - 3457520..3459133 (+) 1614 WP_286069616.1 ATP-binding protein -
  QP512_RS15945 pilR 3459194..3460567 (+) 1374 WP_286069617.1 sigma-54 dependent transcriptional regulator Regulator
  QP512_RS15950 pilB 3460744..3462474 (-) 1731 WP_286069618.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QP512_RS15955 pilA/pilAI 3462697..3463104 (-) 408 WP_286072056.1 pilin Machinery gene
  QP512_RS15960 - 3463257..3463679 (-) 423 WP_049403031.1 pilin -
  QP512_RS15965 pilC 3464032..3465291 (+) 1260 WP_286069619.1 type II secretion system F family protein Machinery gene
  QP512_RS15970 - 3465299..3466162 (+) 864 WP_286069620.1 A24 family peptidase -
  QP512_RS15975 coaE 3466174..3466785 (+) 612 WP_286069621.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62376.33 Da        Isoelectric Point: 6.0438

>NTDB_id=844131 QP512_RS15950 WP_286069618.1 3460744..3462474(-) (pilB) [Stenotrophomonas sp. 57]
MNAVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMNLVSEELLRKHNVLPLFKRGGRLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDAIGDALGGD
DEGMGNLDVAAGDEDLGASSDAGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRRSEFPAH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGITSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=844131 QP512_RS15950 WP_286069618.1 3460744..3462474(-) (pilB) [Stenotrophomonas sp. 57]
ATGAACGCTGTCACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGACGGGGCCCTGGACGA
GGCCTCCGCCCGCGACGCCATGGCCAAGGCCGCGGCCGCCCGCCAGCCACTGCCAACGTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTTTTCGACGTGTCCACCTTCGACGCCAGC
CAGAACGCGATGAACCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGTGGCAGGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAATCTGGTGGTCGAGCCCATCCTGG
TCGATGAAGACCAGATCCGTCGCACGCTGGAGCAGTGGCATGCCAGCCACGACGCGATCGGCGACGCGCTGGGGGGCGAC
GACGAGGGCATGGGCAATCTGGACGTGGCGGCCGGCGACGAGGATCTCGGTGCCAGCAGCGACGCCGGCATCGACGCCAA
GGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCAAGGGTGCCTCGGACATCCATT
TCGAGCCCTACGAAGACGACTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAAATGGTGGCGCGCGCGCCGGTGAAG
TTGAACCAGCGCATTGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGGCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTCGATGGCAGTGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTGTTC
CTGGATGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACCGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGATGAGACGCGCAACATTTCCACTGCCGAAGATCCAGTGGAAATCCGCCTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCTGCGGCACTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCATATGGTGCTGTC
CACGCTGCATACCAACGACGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGAATCGCGCCCTACAACATCACCAGTT
CGGTGACGCTGGTGATCGCCCAGCGATTGGCGCGCCGGCTGTGCGGCAACTGCAAGAGGCGTTCGGAGTTTCCCGCACAT
GCCCTGCTGGCCGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGTATCGAACTGTACGAGGCCGTTGGCTGCGACGAATG
TACCGAGGGCTACAAGGGCCGAACCGGCATCTACCAGGTCATGCCGATGACCGATGAGATCGCCACCATCGTGCTGGCCG
GGGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGCCAGTCGGCATTGAAGAAGGCC
GCGGCAGGTATCACCAGCCTGGCCGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.811

100

0.554
  pilB Acinetobacter baylyi ADP1

53.938

100

0.547
  pilB Legionella pneumophila strain ERS1305867

52.373

98.785

0.517
  pilF Neisseria gonorrhoeae MS11

48.858

98.785

0.483
  pilB Vibrio cholerae strain A1552

48.33

98.785

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

45.629

99.306

0.453
  pilB Vibrio campbellii strain DS40M4

45.694

98.785

0.451
  pilF Thermus thermophilus HB27

37.968

97.396

0.37