Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   P9935_RS25890 Genome accession   NZ_CP143648
Coordinates   5552471..5553964 (+) Length   497 a.a.
NCBI ID   WP_003094357.1    Uniprot ID   A0A0H2ZGR9
Organism   Pseudomonas aeruginosa strain CPO459     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5547471..5558964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P9935_RS25860 (P9935_025860) - 5547683..5548492 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  P9935_RS25865 (P9935_025865) kdsD 5548906..5549886 (+) 981 WP_003134758.1 arabinose-5-phosphate isomerase KdsD -
  P9935_RS25870 (P9935_025870) - 5549886..5550425 (+) 540 WP_003098849.1 HAD family hydrolase -
  P9935_RS25875 (P9935_025875) lptC 5550434..5551006 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  P9935_RS25880 (P9935_025880) lptA 5550993..5551520 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  P9935_RS25885 (P9935_025885) lptB 5551520..5552245 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  P9935_RS25890 (P9935_025890) rpoN/rpoN1 5552471..5553964 (+) 1494 WP_003094357.1 RNA polymerase factor sigma-54 Machinery gene
  P9935_RS25895 (P9935_025895) raiA 5554042..5554350 (+) 309 WP_003094359.1 ribosome-associated translation inhibitor RaiA -
  P9935_RS25900 (P9935_025900) ptsN 5554364..5554828 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  P9935_RS25905 (P9935_025905) rapZ 5554830..5555690 (+) 861 WP_003094361.1 RNase adapter RapZ -
  P9935_RS25910 (P9935_025910) - 5555720..5555992 (+) 273 WP_015648083.1 HPr family phosphocarrier protein -
  P9935_RS25915 (P9935_025915) - 5556023..5557138 (-) 1116 WP_012077216.1 tyrosine-type recombinase/integrase -
  P9935_RS25920 (P9935_025920) - 5557143..5557379 (-) 237 WP_012077217.1 helix-turn-helix transcriptional regulator -
  P9935_RS25925 (P9935_025925) - 5557474..5557704 (+) 231 WP_012077218.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56054.22 Da        Isoelectric Point: 4.4327

>NTDB_id=844130 P9935_RS25890 WP_003094357.1 5552471..5553964(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain CPO459]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=844130 P9935_RS25890 WP_003094357.1 5552471..5553964(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain CPO459]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCCCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCCCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAGTGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTATCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGGATCGGCGCTCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCACGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACCCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZGR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477