Detailed information NTG0842825

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: QRX25_RS12415
• Genome accession: NZ_CP127224
• Coordinates: 2439276..2439890 (-)
• Length: 204 a.a.
• NCBI ID: WP_212098514.1
• Uniprot ID: -
• Organism: Bacillus sp. L381
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2434276..2444890

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QRX25_RS12385 (QRX25_12380)	rpsT	2434576..2434842 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
QRX25_RS12390 (QRX25_12385)	holA	2434860..2435901 (-)	1042	Protein_2395	DNA polymerase III subunit delta	-
QRX25_RS12395 (QRX25_12390)	-	2435941..2436095 (-)	155	Protein_2396	hypothetical protein	-
QRX25_RS12400 (QRX25_12395)	-	2436136..2436270 (+)	135	WP_003152870.1	YqzM family protein	-
QRX25_RS12405 (QRX25_12400)	comEC	2436289..2438640 (-)	2352	WP_212098512.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QRX25_RS12410 (QRX25_12405)	-	2438641..2439210 (-)	570	WP_065981916.1	ComE operon protein 2	-
QRX25_RS12415 (QRX25_12410)	comEA	2439276..2439890 (-)	615	WP_212098514.1	helix-hairpin-helix domain-containing protein	Machinery gene
QRX25_RS12420 (QRX25_12415)	comER	2439950..2440771 (+)	822	WP_065981918.1	late competence protein ComER	-
QRX25_RS12425 (QRX25_12420)	-	2440840..2441577 (-)	738	WP_014470703.1	class I SAM-dependent methyltransferase	-
QRX25_RS12430 (QRX25_12425)	rsfS	2441574..2441930 (-)	357	WP_115997664.1	ribosome silencing factor	-
QRX25_RS12435 (QRX25_12430)	yqeK	2441947..2442507 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
QRX25_RS12440 (QRX25_12435)	-	2442497..2443066 (-)	570	WP_065981920.1	nicotinate-nucleotide adenylyltransferase	-
QRX25_RS12445 (QRX25_12440)	yhbY	2443077..2443367 (-)	291	WP_014470707.1	ribosome assembly RNA-binding protein YhbY	-
QRX25_RS12450 (QRX25_12445)	aroE	2443361..2444203 (-)	843	WP_115997665.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22138.41 Da
• Isoelectric Point: 6.9665

>NTDB_id=842825 QRX25_RS12415 WP_212098514.1 2439276..2439890(-) (comEA) [Bacillus sp. L381]
MTKVMMTERQRQYLKILGVCLAAGAVLICILLFRPEKEEPVKQTSVLGQTASAPVTDKKDTADDKIMIDIKGAVRHPGVY
EMKAGDRVTQAIKKAGGLKEKADELNVNLAEQLQDGTVVYIPSEGEKENPPKTAAGGKENASININTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=842825 QRX25_RS12415 WP_212098514.1 2439276..2439890(-) (comEA) [Bacillus sp. L381]
ATGACGAAAGTGATGATGACGGAGCGGCAGAGGCAGTACCTGAAAATTCTTGGCGTTTGTCTTGCGGCAGGGGCGGTTTT
GATCTGCATTTTGCTGTTTCGTCCCGAAAAAGAAGAGCCTGTCAAACAGACGTCCGTTCTCGGCCAAACAGCCTCAGCAC
CCGTAACGGACAAAAAAGACACCGCAGACGATAAAATTATGATTGATATTAAAGGAGCGGTCCGACATCCCGGCGTGTAT
GAAATGAAGGCGGGAGACAGAGTGACGCAAGCCATTAAGAAAGCCGGCGGCCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGAACGGTCGTTTATATCCCGAGTGAAGGCGAAAAGGAGAATCCCCCGAAAACCG
CGGCAGGTGGAAAGGAGAATGCTTCTATCAATATCAATACGGCATCTCTGGATGAACTTCAGGCGATTTCCGGAGTGGGA
CAGAAAAAAGCGGAAGCGATTATTGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	57.949	95.588	0.554
comEA/celA/cilE	Streptococcus mitis SK321	35.945	100	0.382
comEA	Staphylococcus aureus MW2	34.842	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae R6	35.484	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae D39	35.484	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.484	100	0.377
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.484	100	0.377
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.023	100	0.373
comEA	Staphylococcus aureus N315	33.937	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	32.051	100	0.368