Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   VMR32_RS23985 Genome accession   NZ_CP142601
Coordinates   5158117..5159334 (+) Length   405 a.a.
NCBI ID   WP_034014663.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain Tongji     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5153117..5164334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VMR32_RS23965 (VMR32_23965) nadC 5154667..5155515 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VMR32_RS23975 (VMR32_23975) pilA 5155719..5156183 (-) 465 WP_031630343.1 pilin Machinery gene
  VMR32_RS23980 (VMR32_23980) pilB 5156413..5158113 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VMR32_RS23985 (VMR32_23985) pilC 5158117..5159334 (+) 1218 WP_034014663.1 type II secretion system F family protein Machinery gene
  VMR32_RS23990 (VMR32_23990) pilD 5159335..5160207 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  VMR32_RS23995 (VMR32_23995) coaE 5160204..5160815 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  VMR32_RS24000 (VMR32_24000) yacG 5160812..5161012 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  VMR32_RS24005 (VMR32_24005) - 5161049..5161258 (-) 210 WP_003094660.1 hypothetical protein -
  VMR32_RS24010 (VMR32_24010) - 5161364..5162053 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  VMR32_RS24015 (VMR32_24015) - 5162050..5162520 (-) 471 WP_003094664.1 hypothetical protein -
  VMR32_RS24020 (VMR32_24020) - 5162517..5162942 (-) 426 WP_016562341.1 GNAT family N-acetyltransferase -
  VMR32_RS24025 (VMR32_24025) - 5163075..5163704 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  VMR32_RS24030 (VMR32_24030) - 5163701..5164150 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44200.03 Da        Isoelectric Point: 9.8572

>NTDB_id=841504 VMR32_RS23985 WP_034014663.1 5158117..5159334(+) (pilC) [Pseudomonas aeruginosa strain Tongji]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFESFGAELPAFTQMVINISTVLQEWWILVLLFLGGAGFLLK
YFYKRSEKFRNNTDRAVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVDKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=841504 VMR32_RS23985 WP_034014663.1 5158117..5159334(+) (pilC) [Pseudomonas aeruginosa strain Tongji]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TTTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAATCCGTATTTGAAAGCTTCGGCGCAGAACTCCCCGCGTTCACCCAAATGG
TCATCAATATATCAACAGTTCTTCAAGAATGGTGGATTCTGGTTCTACTGTTTTTGGGCGGAGCCGGTTTCCTCCTTAAA
TATTTTTATAAACGTTCGGAGAAATTCAGAAACAACACTGATCGCGCAGTGCTAAAGCTGCCGATCGTCGGCGCTATCCT
CTACAAGTCCGCCGTGGCACGCTACGCGCGGACACTATCGACCACCTTCGCCGCCGGCGTACCACTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGAGATGCGGTGGACAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.012

100

0.79
  pilC Acinetobacter baylyi ADP1

60.784

100

0.612
  pilC Acinetobacter baumannii D1279779

60.396

99.753

0.602
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilG Neisseria meningitidis 44/76-A

46.02

99.259

0.457
  pilG Neisseria gonorrhoeae MS11

45.771

99.259

0.454
  pilC Vibrio cholerae strain A1552

41.278

100

0.415
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

37.656

99.012

0.373