Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QPX65_RS12665 Genome accession   NZ_CP126698
Coordinates   2665478..2667166 (+) Length   562 a.a.
NCBI ID   WP_285454503.1    Uniprot ID   -
Organism   Vibrio vulnificus strain VV2018     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2660478..2672166
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPX65_RS12645 (QPX65_12645) pdhR 2662109..2662876 (-) 768 WP_047108811.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  QPX65_RS12650 (QPX65_12650) ampD 2663265..2663837 (-) 573 WP_285454496.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QPX65_RS12655 (QPX65_12655) nadC 2663930..2664817 (+) 888 WP_013571142.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QPX65_RS12660 (QPX65_12660) - 2665040..2665468 (+) 429 WP_224655347.1 pilin -
  QPX65_RS12665 (QPX65_12665) pilB 2665478..2667166 (+) 1689 WP_285454503.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QPX65_RS12670 (QPX65_12670) pilC 2667214..2668440 (+) 1227 WP_013571139.1 type II secretion system F family protein Machinery gene
  QPX65_RS12675 (QPX65_12675) pilD 2668534..2669403 (+) 870 WP_285454506.1 A24 family peptidase Machinery gene
  QPX65_RS12680 (QPX65_12680) coaE 2669405..2670013 (+) 609 WP_285454508.1 dephospho-CoA kinase -
  QPX65_RS12685 (QPX65_12685) zapD 2670046..2670786 (+) 741 WP_011079546.1 cell division protein ZapD -
  QPX65_RS12690 (QPX65_12690) yacG 2670849..2671043 (+) 195 WP_011079545.1 DNA gyrase inhibitor YacG -
  QPX65_RS12695 (QPX65_12695) rplS 2671251..2671604 (-) 354 WP_011079544.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62414.43 Da        Isoelectric Point: 5.5349

>NTDB_id=839648 QPX65_RS12665 WP_285454503.1 2665478..2667166(+) (pilB) [Vibrio vulnificus strain VV2018]
MISNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQGSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=839648 QPX65_RS12665 WP_285454503.1 2665478..2667166(+) (pilB) [Vibrio vulnificus strain VV2018]
ATGATATCCAATCTTGCCACTATTCTGCGTCAGGCCAACCAACTTAGCCTAACGCAAGAGCAAGCGTGTCGAGAGACAAT
ACAAGCTTCCGGTGTCACAGCACCGGAAGCTTTGTTGCAACTGGGCTTTTTCCAGCCTGATGAACTAACCGAACAGCTAA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTGCGTGAG
CTGATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCGACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGCGTGCAGGTGGAGATTGTTTTGGCCGATTGCCAAGAGCTGCAAGCGG
CGATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAGGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTGGTTGAGGTTTCAGATGACGAAGTCGAGTCTATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGCTTTAT
CAACCAAATCCTGCTTGATGCGGTACGTAAAGGCGCGTCCGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAG
ATCCTCTCCAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGTA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTGCTTAACCGGCCAGAAGT
GAATATTTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGTCACCTTGTGCTATCCACCTTACATACCAACTCGGCTGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGCCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAGACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAACAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTAGAGATGCTTGCTCGACAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGTTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.512

100

0.765
  pilB Vibrio parahaemolyticus RIMD 2210633

75.979

100

0.76
  pilB Vibrio cholerae strain A1552

74.423

100

0.746
  pilB Legionella pneumophila strain ERS1305867

53.216

91.281

0.486
  pilB Acinetobacter baumannii D1279779

51.538

92.527

0.477
  pilB Acinetobacter baylyi ADP1

50.478

93.06

0.47
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

40.787

99.466

0.406
  pilB/pilB1 Synechocystis sp. PCC 6803

36.888

100

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363