Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QPK06_RS11885 Genome accession   NZ_CP126578
Coordinates   2496585..2498291 (-) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain AV066     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2491585..2503291
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QPK06_RS11855 (QPK06_11855) mutT 2492097..2492501 (+) 405 WP_042053534.1 8-oxo-dGTP diphosphatase MutT -
  QPK06_RS11860 (QPK06_11860) yacG 2492579..2492773 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  QPK06_RS11865 (QPK06_11865) zapD 2492783..2493505 (-) 723 WP_005336141.1 cell division protein ZapD -
  QPK06_RS11870 (QPK06_11870) coaE 2493543..2494157 (-) 615 WP_124928361.1 dephospho-CoA kinase -
  QPK06_RS11875 (QPK06_11875) pilD 2494171..2495043 (-) 873 WP_124928362.1 A24 family peptidase Machinery gene
  QPK06_RS11880 (QPK06_11880) pilC 2495105..2496346 (-) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  QPK06_RS11885 (QPK06_11885) pilB 2496585..2498291 (-) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  QPK06_RS11890 (QPK06_11890) - 2498294..2498755 (-) 462 WP_139742528.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QPK06_RS11895 (QPK06_11895) nadC 2499071..2499934 (-) 864 WP_043821973.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QPK06_RS11900 (QPK06_11900) - 2499938..2500408 (-) 471 WP_040068932.1 retropepsin-like aspartic protease -
  QPK06_RS11905 (QPK06_11905) ampD 2500539..2501114 (+) 576 WP_139742527.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QPK06_RS11910 (QPK06_11910) pdhR 2501393..2502157 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=838929 QPK06_RS11885 WP_005336134.1 2496585..2498291(-) (pilB) [Aeromonas veronii strain AV066]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=838929 QPK06_RS11885 WP_005336134.1 2496585..2498291(-) (pilB) [Aeromonas veronii strain AV066]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGTCTTCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAACGACGCCCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCGGATCTCGAGGTCTCCGACGAGAACAGCCGGCTGGACGAAAGCGTCAATACCGCCGATGACGATGCCCCCATAGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGTGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGACGGCATTCTGCATGAAATCGCTACACCACCGGTCAACCTCTCCAACCGATTCTCCGCCCGC
CTCAAGGTGATGGCACGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAACTGAAGCTGTCACGCAA
CAAATCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGATCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAACCACAG
GGGATGATCCTGGTGACTGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACTAC
CGAGGTAAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACTTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCTTGCGCTCCTTCCTGCGGCAAGATCCGGATGTGGTGATGGTGGGGGAAATACGGGAT
CTCGAAACCGCCGAGATCGCTATCAAGGCGGCGCAGACCGGTCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTACCGGCCTTCAACATCGCCTCCTCGGTGACCTTGATCATGGCTCAGC
GGCTGGCCCGTAAACTATGCGACCACTGCAAAGCGCCGGAAGTAGTGCCGGAAGCAGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAACTGGCGGCCGGGCTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGCTGATGTCAGAGAACATCGCCAAATTGATCATGCAGGGGGCCAACTCCCTGCAAATCGCCG
CTATAGCCCAGAAGGAGGGGATGCGCACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377